Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2A6H

Crystal structure of the T. thermophilus RNA polymerase holoenzyme in complex with antibiotic sterptolydigin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed RNA polymerase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006351	biological_process	DNA-templated transcription
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0046983	molecular_function	protein dimerization activity
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed RNA polymerase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006351	biological_process	DNA-templated transcription
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0046983	molecular_function	protein dimerization activity
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed RNA polymerase activity
C	0006351	biological_process	DNA-templated transcription
C	0016740	molecular_function	transferase activity
C	0016779	molecular_function	nucleotidyltransferase activity
C	0032549	molecular_function	ribonucleoside binding
C	0034062	molecular_function	5'-3' RNA polymerase activity
D	0000287	molecular_function	magnesium ion binding
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed RNA polymerase activity
D	0005515	molecular_function	protein binding
D	0006351	biological_process	DNA-templated transcription
D	0008270	molecular_function	zinc ion binding
D	0016740	molecular_function	transferase activity
D	0016779	molecular_function	nucleotidyltransferase activity
D	0034062	molecular_function	5'-3' RNA polymerase activity
D	0046872	molecular_function	metal ion binding
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed RNA polymerase activity
E	0006351	biological_process	DNA-templated transcription
E	0016740	molecular_function	transferase activity
E	0016779	molecular_function	nucleotidyltransferase activity
E	0034062	molecular_function	5'-3' RNA polymerase activity
F	0003677	molecular_function	DNA binding
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0005737	cellular_component	cytoplasm
F	0006352	biological_process	DNA-templated transcription initiation
F	0006355	biological_process	regulation of DNA-templated transcription
F	0010468	biological_process	regulation of gene expression
F	0016987	molecular_function	sigma factor activity
F	0046872	molecular_function	metal ion binding
F	2000142	biological_process	regulation of DNA-templated transcription initiation
K	0000428	cellular_component	DNA-directed RNA polymerase complex
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed RNA polymerase activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0006351	biological_process	DNA-templated transcription
K	0016740	molecular_function	transferase activity
K	0016779	molecular_function	nucleotidyltransferase activity
K	0034062	molecular_function	5'-3' RNA polymerase activity
K	0046983	molecular_function	protein dimerization activity
L	0000428	cellular_component	DNA-directed RNA polymerase complex
L	0003677	molecular_function	DNA binding
L	0003899	molecular_function	DNA-directed RNA polymerase activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0006351	biological_process	DNA-templated transcription
L	0016740	molecular_function	transferase activity
L	0016779	molecular_function	nucleotidyltransferase activity
L	0034062	molecular_function	5'-3' RNA polymerase activity
L	0046983	molecular_function	protein dimerization activity
M	0000428	cellular_component	DNA-directed RNA polymerase complex
M	0003677	molecular_function	DNA binding
M	0003899	molecular_function	DNA-directed RNA polymerase activity
M	0006351	biological_process	DNA-templated transcription
M	0016740	molecular_function	transferase activity
M	0016779	molecular_function	nucleotidyltransferase activity
M	0032549	molecular_function	ribonucleoside binding
M	0034062	molecular_function	5'-3' RNA polymerase activity
N	0000287	molecular_function	magnesium ion binding
N	0000428	cellular_component	DNA-directed RNA polymerase complex
N	0003677	molecular_function	DNA binding
N	0003899	molecular_function	DNA-directed RNA polymerase activity
N	0005515	molecular_function	protein binding
N	0006351	biological_process	DNA-templated transcription
N	0008270	molecular_function	zinc ion binding
N	0016740	molecular_function	transferase activity
N	0016779	molecular_function	nucleotidyltransferase activity
N	0034062	molecular_function	5'-3' RNA polymerase activity
N	0046872	molecular_function	metal ion binding
O	0000428	cellular_component	DNA-directed RNA polymerase complex
O	0003677	molecular_function	DNA binding
O	0003899	molecular_function	DNA-directed RNA polymerase activity
O	0006351	biological_process	DNA-templated transcription
O	0016740	molecular_function	transferase activity
O	0016779	molecular_function	nucleotidyltransferase activity
O	0034062	molecular_function	5'-3' RNA polymerase activity
P	0003677	molecular_function	DNA binding
P	0003700	molecular_function	DNA-binding transcription factor activity
P	0005737	cellular_component	cytoplasm
P	0006352	biological_process	DNA-templated transcription initiation
P	0006355	biological_process	regulation of DNA-templated transcription
P	0010468	biological_process	regulation of gene expression
P	0016987	molecular_function	sigma factor activity
P	0046872	molecular_function	metal ion binding
P	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE STD D 8001

Chain	Residue
C	ALA423
D	HOH2145
D	HOH2274
D	HOH9003
D	HOH9021
D	HOH9121
D	HOH9360
D	HOH9645
C	PHE425
C	ARG428
C	ILE449
D	ALA1082
D	ALA1085
D	LEU1086
D	ASP1090
D	PRO1257

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 7458

Chain	Residue
D	CYS58
D	CYS60
D	CYS73
D	CYS76

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN D 7412

Chain	Residue
D	CYS1112
D	ARG1189
D	CYS1194
D	CYS1201
D	CYS1204

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 9001

Chain	Residue
D	ASP739
D	ASP741
D	ASP743

site_id	AC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE STD N 8002

Chain	Residue
M	ALA423
M	PHE425
M	ARG428
M	ILE449
M	HOH1712
N	ALA1082
N	ALA1085
N	LEU1086
N	ASP1090
N	PRO1257
N	HOH2340
N	HOH9013
N	HOH9040
N	HOH9390

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN N 7459

Chain	Residue
N	CYS58
N	CYS60
N	CYS73
N	CYS76

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN N 7413

Chain	Residue
N	CYS1112
N	ARG1189
N	CYS1194
N	CYS1201
N	CYS1204
N	HOH9751

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG N 9002

Chain	Residue
N	ASP739
N	ASP741
N	ASP743

Functional Information from PROSITE/UniProt

site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TleEVGaffgVTrerIrQIEnkaLrkL

Chain	Residue	Details
F	THR382-LEU408

site_id	PS00867
Number of Residues	8
Details	CPSASE_2 Carbamoyl-phosphate synthase subdomain signature 2. LIEANLRL

Chain	Residue	Details
F	LEU187-LEU194

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKLANrHGNKGV

Chain	Residue	Details
C	GLY836-VAL848

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	912
Details	Region: {"description":"Alpha N-terminal domain (alpha-NTD)","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	22
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)