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2A5D

Structural basis for the activation of cholera toxin by human ARF6-GTP

Functional Information from GO Data
ChainGOidnamespacecontents
A0000166molecular_functionnucleotide binding
A0000281biological_processmitotic cytokinesis
A0001726cellular_componentruffle
A0001889biological_processliver development
A0003924molecular_functionGTPase activity
A0003925molecular_functionG protein activity
A0005515molecular_functionprotein binding
A0005525molecular_functionGTP binding
A0005737cellular_componentcytoplasm
A0005768cellular_componentendosome
A0005769cellular_componentearly endosome
A0005794cellular_componentGolgi apparatus
A0005829cellular_componentcytosol
A0005886cellular_componentplasma membrane
A0005925cellular_componentfocal adhesion
A0005938cellular_componentcell cortex
A0006886biological_processintracellular protein transport
A0006904biological_processvesicle docking involved in exocytosis
A0007155biological_processcell adhesion
A0007399biological_processnervous system development
A0010008cellular_componentendosome membrane
A0010975biological_processregulation of neuron projection development
A0010976biological_processpositive regulation of neuron projection development
A0015031biological_processprotein transport
A0016020cellular_componentmembrane
A0016192biological_processvesicle-mediated transport
A0016787molecular_functionhydrolase activity
A0019003molecular_functionGDP binding
A0030139cellular_componentendocytic vesicle
A0030154biological_processcell differentiation
A0030496cellular_componentmidbody
A0030838biological_processpositive regulation of actin filament polymerization
A0030866biological_processcortical actin cytoskeleton organization
A0031527cellular_componentfilopodium membrane
A0031901cellular_componentearly endosome membrane
A0031996molecular_functionthioesterase binding
A0032154cellular_componentcleavage furrow
A0032456biological_processendocytic recycling
A0034394biological_processprotein localization to cell surface
A0035020biological_processregulation of Rac protein signal transduction
A0035591molecular_functionsignaling adaptor activity
A0036010biological_processprotein localization to endosome
A0042995cellular_componentcell projection
A0048261biological_processnegative regulation of receptor-mediated endocytosis
A0048488biological_processsynaptic vesicle endocytosis
A0050714biological_processpositive regulation of protein secretion
A0051301biological_processcell division
A0051489biological_processregulation of filopodium assembly
A0051549biological_processpositive regulation of keratinocyte migration
A0055037cellular_componentrecycling endosome
A0055038cellular_componentrecycling endosome membrane
A0060998biological_processregulation of dendritic spine development
A0070062cellular_componentextracellular exosome
A0071726biological_processcellular response to diacyl bacterial lipopeptide
A0072659biological_processprotein localization to plasma membrane
A0090162biological_processestablishment of epithelial cell polarity
A0090543cellular_componentFlemming body
A0097178biological_processruffle assembly
A0097284biological_processhepatocyte apoptotic process
A0098793cellular_componentpresynapse
A0098794cellular_componentpostsynapse
A0098978cellular_componentglutamatergic synapse
A0099562biological_processmaintenance of postsynaptic density structure
A0120183biological_processpositive regulation of focal adhesion disassembly
A1902217biological_processerythrocyte apoptotic process
A1903078biological_processpositive regulation of protein localization to plasma membrane
A1903438biological_processpositive regulation of mitotic cytokinetic process
A1905345biological_processprotein localization to cleavage furrow
A1905606biological_processregulation of presynapse assembly
A1990090biological_processcellular response to nerve growth factor stimulus
A2000009biological_processnegative regulation of protein localization to cell surface
A2000171biological_processnegative regulation of dendrite development
B0005615cellular_componentextracellular space
B0090729molecular_functiontoxin activity
Functional Information from PDB Data
site_idAC1
Number of Residues5
DetailsBINDING SITE FOR RESIDUE NA B 326
ChainResidue
BASN1
BTHR90
BTYR150
BLEU153
BHOH270

site_idAC2
Number of Residues5
DetailsBINDING SITE FOR RESIDUE MG A 1231
ChainResidue
AHOH1233
ATHR27
ATHR44
AGTP1230
AHOH1232

site_idAC3
Number of Residues28
DetailsBINDING SITE FOR RESIDUE GTP A 1230
ChainResidue
AASP22
AALA23
AALA24
AGLY25
ALYS26
ATHR27
ATHR28
ATHR41
ATHR44
AGLY65
AGLY66
AASN122
ALYS123
AASP125
ALEU126
ACYS155
AALA156
ATHR157
AHOH200
AHOH238
AHOH281
AMG1231
AHOH1232
AHOH1233
AHOH1268
AHOH1322
AHOH1342
AHOH1345

site_idAC4
Number of Residues10
DetailsBINDING SITE FOR RESIDUE GOL B 1496
ChainResidue
ALYS69
APRO72
BALA159
BASP160
BTYR162
BGLY163
BHIS182
BHOH1308
BHOH1313
BHOH1393

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1
DetailsACT_SITE: ACT_SITE => ECO:0000250
ChainResidueDetails
BASP112
AILE42
AVAL64
ALYS123
AALA156

site_idSWS_FT_FI2
Number of Residues2
DetailsBINDING: BINDING => ECO:0000269|PubMed:16099990
ChainResidueDetails
BARG7
BMET23

site_idSWS_FT_FI3
Number of Residues1
DetailsLIPID: N6-myristoyl lysine => ECO:0000269|PubMed:32103017
ChainResidueDetails
AVAL4

Catalytic Information from CSA
site_idCSA1
Number of Residues4
DetailsAnnotated By Reference To The Literature 1xtc
ChainResidueDetails
BSER61
BARG7
BASP112
BASP110

site_idCSA2
Number of Residues1
DetailsAnnotated By Reference To The Literature 1xtc
ChainResidueDetails
AGLN67

site_idMCSA1
Number of Residues4
DetailsM-CSA 919
ChainResidueDetails
BARG7electrostatic stabiliser
BSER61electrostatic stabiliser
BASP110proton shuttle (general acid/base)
BASP112electrostatic stabiliser

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PDB entries from 2025-06-11

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