Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1YOV

Insights into the Ubiquitin Transfer Cascade from the refined structure of the activating enzyme for NEDD8

Replaces: 1NGV

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006915	biological_process	apoptotic process
A	0007165	biological_process	signal transduction
A	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
A	0016020	cellular_component	membrane
A	0019781	molecular_function	NEDD8 activating enzyme activity
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0032991	cellular_component	protein-containing complex
A	0033314	biological_process	mitotic DNA replication checkpoint signaling
A	0042981	biological_process	regulation of apoptotic process
A	0043523	biological_process	regulation of neuron apoptotic process
A	0045116	biological_process	protein neddylation
A	0046982	molecular_function	protein heterodimerization activity
A	0051402	biological_process	neuron apoptotic process
A	0098978	cellular_component	glutamatergic synapse
A	0150052	biological_process	regulation of postsynapse assembly
B	0000166	molecular_function	nucleotide binding
B	0000278	biological_process	mitotic cell cycle
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006508	biological_process	proteolysis
B	0007113	biological_process	endomitotic cell cycle
B	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
B	0016874	molecular_function	ligase activity
B	0019781	molecular_function	NEDD8 activating enzyme activity
B	0019788	molecular_function	NEDD8 transferase activity
B	0032991	cellular_component	protein-containing complex
B	0036211	biological_process	protein modification process
B	0042802	molecular_function	identical protein binding
B	0043687	biological_process	post-translational protein modification
B	0045116	biological_process	protein neddylation
B	0046982	molecular_function	protein heterodimerization activity
B	0051726	biological_process	regulation of cell cycle
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006915	biological_process	apoptotic process
C	0007165	biological_process	signal transduction
C	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
C	0016020	cellular_component	membrane
C	0019781	molecular_function	NEDD8 activating enzyme activity
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0032991	cellular_component	protein-containing complex
C	0033314	biological_process	mitotic DNA replication checkpoint signaling
C	0042981	biological_process	regulation of apoptotic process
C	0043523	biological_process	regulation of neuron apoptotic process
C	0045116	biological_process	protein neddylation
C	0046982	molecular_function	protein heterodimerization activity
C	0051402	biological_process	neuron apoptotic process
C	0098978	cellular_component	glutamatergic synapse
C	0150052	biological_process	regulation of postsynapse assembly
D	0000166	molecular_function	nucleotide binding
D	0000278	biological_process	mitotic cell cycle
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006508	biological_process	proteolysis
D	0007113	biological_process	endomitotic cell cycle
D	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
D	0016874	molecular_function	ligase activity
D	0019781	molecular_function	NEDD8 activating enzyme activity
D	0019788	molecular_function	NEDD8 transferase activity
D	0032991	cellular_component	protein-containing complex
D	0036211	biological_process	protein modification process
D	0042802	molecular_function	identical protein binding
D	0043687	biological_process	post-translational protein modification
D	0045116	biological_process	protein neddylation
D	0046982	molecular_function	protein heterodimerization activity
D	0051726	biological_process	regulation of cell cycle

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 443

Chain	Residue
B	CYS199
B	CYS202
B	CYS343
B	CYS346

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 443

Chain	Residue
D	CYS199
D	CYS202
D	CYS343
D	CYS346

Functional Information from PROSITE/UniProt

site_id	PS00865
Number of Residues	9
Details	UBIQUITIN_ACTIVAT_2 Ubiquitin-activating enzyme active site. PMCTIAsMP

Chain	Residue	Details
B	PRO214-PRO222

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	Region: {"description":"Interaction with UBA3"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	2
Details	Site: {"description":"Interaction with UBA3"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q8VBW6","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	106
Details	Region: {"description":"Interaction with UBE2M N-terminus"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	14
Details	Region: {"description":"Interaction with NEDD8"}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	18
Details	Region: {"description":"Interaction with NAE1","evidences":[{"source":"PubMed","id":"12740388","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	2
Details	Active site: {"description":"Glycyl thioester intermediate"}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	94
Details	Binding site: {"evidences":[{"source":"PubMed","id":"14690597","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	2
Details	Site: {"description":"Determines specificity for NEDD8"}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)