1YAJ

Crystal Structure of Human Liver Carboxylesterase in complex with benzil

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004771	molecular_function	sterol esterase activity
A	0005737	cellular_component	cytoplasm
A	0005783	cellular_component	endoplasmic reticulum
A	0005788	cellular_component	endoplasmic reticulum lumen
A	0005811	cellular_component	lipid droplet
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0006695	biological_process	cholesterol biosynthetic process
A	0008203	biological_process	cholesterol metabolic process
A	0009636	biological_process	response to toxic substance
A	0010875	biological_process	positive regulation of cholesterol efflux
A	0010887	biological_process	negative regulation of cholesterol storage
A	0016042	biological_process	lipid catabolic process
A	0030855	biological_process	epithelial cell differentiation
A	0042632	biological_process	cholesterol homeostasis
A	0043691	biological_process	reverse cholesterol transport
A	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
A	0051791	biological_process	medium-chain fatty acid metabolic process
A	0052689	molecular_function	carboxylic ester hydrolase activity
A	0070857	biological_process	regulation of bile acid biosynthetic process
A	0071397	biological_process	cellular response to cholesterol
A	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
A	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
A	0090205	biological_process	positive regulation of cholesterol metabolic process
A	0106435	molecular_function	carboxylesterase activity
A	0120188	biological_process	regulation of bile acid secretion
B	0004771	molecular_function	sterol esterase activity
B	0005737	cellular_component	cytoplasm
B	0005783	cellular_component	endoplasmic reticulum
B	0005788	cellular_component	endoplasmic reticulum lumen
B	0005811	cellular_component	lipid droplet
B	0005829	cellular_component	cytosol
B	0006629	biological_process	lipid metabolic process
B	0006695	biological_process	cholesterol biosynthetic process
B	0008203	biological_process	cholesterol metabolic process
B	0009636	biological_process	response to toxic substance
B	0010875	biological_process	positive regulation of cholesterol efflux
B	0010887	biological_process	negative regulation of cholesterol storage
B	0016042	biological_process	lipid catabolic process
B	0030855	biological_process	epithelial cell differentiation
B	0042632	biological_process	cholesterol homeostasis
B	0043691	biological_process	reverse cholesterol transport
B	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
B	0051791	biological_process	medium-chain fatty acid metabolic process
B	0052689	molecular_function	carboxylic ester hydrolase activity
B	0070857	biological_process	regulation of bile acid biosynthetic process
B	0071397	biological_process	cellular response to cholesterol
B	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
B	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
B	0090205	biological_process	positive regulation of cholesterol metabolic process
B	0106435	molecular_function	carboxylesterase activity
B	0120188	biological_process	regulation of bile acid secretion
C	0004771	molecular_function	sterol esterase activity
C	0005737	cellular_component	cytoplasm
C	0005783	cellular_component	endoplasmic reticulum
C	0005788	cellular_component	endoplasmic reticulum lumen
C	0005811	cellular_component	lipid droplet
C	0005829	cellular_component	cytosol
C	0006629	biological_process	lipid metabolic process
C	0006695	biological_process	cholesterol biosynthetic process
C	0008203	biological_process	cholesterol metabolic process
C	0009636	biological_process	response to toxic substance
C	0010875	biological_process	positive regulation of cholesterol efflux
C	0010887	biological_process	negative regulation of cholesterol storage
C	0016042	biological_process	lipid catabolic process
C	0030855	biological_process	epithelial cell differentiation
C	0042632	biological_process	cholesterol homeostasis
C	0043691	biological_process	reverse cholesterol transport
C	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
C	0051791	biological_process	medium-chain fatty acid metabolic process
C	0052689	molecular_function	carboxylic ester hydrolase activity
C	0070857	biological_process	regulation of bile acid biosynthetic process
C	0071397	biological_process	cellular response to cholesterol
C	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
C	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
C	0090205	biological_process	positive regulation of cholesterol metabolic process
C	0106435	molecular_function	carboxylesterase activity
C	0120188	biological_process	regulation of bile acid secretion
D	0004771	molecular_function	sterol esterase activity
D	0005737	cellular_component	cytoplasm
D	0005783	cellular_component	endoplasmic reticulum
D	0005788	cellular_component	endoplasmic reticulum lumen
D	0005811	cellular_component	lipid droplet
D	0005829	cellular_component	cytosol
D	0006629	biological_process	lipid metabolic process
D	0006695	biological_process	cholesterol biosynthetic process
D	0008203	biological_process	cholesterol metabolic process
D	0009636	biological_process	response to toxic substance
D	0010875	biological_process	positive regulation of cholesterol efflux
D	0010887	biological_process	negative regulation of cholesterol storage
D	0016042	biological_process	lipid catabolic process
D	0030855	biological_process	epithelial cell differentiation
D	0042632	biological_process	cholesterol homeostasis
D	0043691	biological_process	reverse cholesterol transport
D	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
D	0051791	biological_process	medium-chain fatty acid metabolic process
D	0052689	molecular_function	carboxylic ester hydrolase activity
D	0070857	biological_process	regulation of bile acid biosynthetic process
D	0071397	biological_process	cellular response to cholesterol
D	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
D	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
D	0090205	biological_process	positive regulation of cholesterol metabolic process
D	0106435	molecular_function	carboxylesterase activity
D	0120188	biological_process	regulation of bile acid secretion
E	0004771	molecular_function	sterol esterase activity
E	0005737	cellular_component	cytoplasm
E	0005783	cellular_component	endoplasmic reticulum
E	0005788	cellular_component	endoplasmic reticulum lumen
E	0005811	cellular_component	lipid droplet
E	0005829	cellular_component	cytosol
E	0006629	biological_process	lipid metabolic process
E	0006695	biological_process	cholesterol biosynthetic process
E	0008203	biological_process	cholesterol metabolic process
E	0009636	biological_process	response to toxic substance
E	0010875	biological_process	positive regulation of cholesterol efflux
E	0010887	biological_process	negative regulation of cholesterol storage
E	0016042	biological_process	lipid catabolic process
E	0030855	biological_process	epithelial cell differentiation
E	0042632	biological_process	cholesterol homeostasis
E	0043691	biological_process	reverse cholesterol transport
E	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
E	0051791	biological_process	medium-chain fatty acid metabolic process
E	0052689	molecular_function	carboxylic ester hydrolase activity
E	0070857	biological_process	regulation of bile acid biosynthetic process
E	0071397	biological_process	cellular response to cholesterol
E	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
E	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
E	0090205	biological_process	positive regulation of cholesterol metabolic process
E	0106435	molecular_function	carboxylesterase activity
E	0120188	biological_process	regulation of bile acid secretion
F	0004771	molecular_function	sterol esterase activity
F	0005737	cellular_component	cytoplasm
F	0005783	cellular_component	endoplasmic reticulum
F	0005788	cellular_component	endoplasmic reticulum lumen
F	0005811	cellular_component	lipid droplet
F	0005829	cellular_component	cytosol
F	0006629	biological_process	lipid metabolic process
F	0006695	biological_process	cholesterol biosynthetic process
F	0008203	biological_process	cholesterol metabolic process
F	0009636	biological_process	response to toxic substance
F	0010875	biological_process	positive regulation of cholesterol efflux
F	0010887	biological_process	negative regulation of cholesterol storage
F	0016042	biological_process	lipid catabolic process
F	0030855	biological_process	epithelial cell differentiation
F	0042632	biological_process	cholesterol homeostasis
F	0043691	biological_process	reverse cholesterol transport
F	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
F	0051791	biological_process	medium-chain fatty acid metabolic process
F	0052689	molecular_function	carboxylic ester hydrolase activity
F	0070857	biological_process	regulation of bile acid biosynthetic process
F	0071397	biological_process	cellular response to cholesterol
F	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
F	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
F	0090205	biological_process	positive regulation of cholesterol metabolic process
F	0106435	molecular_function	carboxylesterase activity
F	0120188	biological_process	regulation of bile acid secretion
G	0004771	molecular_function	sterol esterase activity
G	0005737	cellular_component	cytoplasm
G	0005783	cellular_component	endoplasmic reticulum
G	0005788	cellular_component	endoplasmic reticulum lumen
G	0005811	cellular_component	lipid droplet
G	0005829	cellular_component	cytosol
G	0006629	biological_process	lipid metabolic process
G	0006695	biological_process	cholesterol biosynthetic process
G	0008203	biological_process	cholesterol metabolic process
G	0009636	biological_process	response to toxic substance
G	0010875	biological_process	positive regulation of cholesterol efflux
G	0010887	biological_process	negative regulation of cholesterol storage
G	0016042	biological_process	lipid catabolic process
G	0030855	biological_process	epithelial cell differentiation
G	0042632	biological_process	cholesterol homeostasis
G	0043691	biological_process	reverse cholesterol transport
G	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
G	0051791	biological_process	medium-chain fatty acid metabolic process
G	0052689	molecular_function	carboxylic ester hydrolase activity
G	0070857	biological_process	regulation of bile acid biosynthetic process
G	0071397	biological_process	cellular response to cholesterol
G	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
G	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
G	0090205	biological_process	positive regulation of cholesterol metabolic process
G	0106435	molecular_function	carboxylesterase activity
G	0120188	biological_process	regulation of bile acid secretion
H	0004771	molecular_function	sterol esterase activity
H	0005737	cellular_component	cytoplasm
H	0005783	cellular_component	endoplasmic reticulum
H	0005788	cellular_component	endoplasmic reticulum lumen
H	0005811	cellular_component	lipid droplet
H	0005829	cellular_component	cytosol
H	0006629	biological_process	lipid metabolic process
H	0006695	biological_process	cholesterol biosynthetic process
H	0008203	biological_process	cholesterol metabolic process
H	0009636	biological_process	response to toxic substance
H	0010875	biological_process	positive regulation of cholesterol efflux
H	0010887	biological_process	negative regulation of cholesterol storage
H	0016042	biological_process	lipid catabolic process
H	0030855	biological_process	epithelial cell differentiation
H	0042632	biological_process	cholesterol homeostasis
H	0043691	biological_process	reverse cholesterol transport
H	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
H	0051791	biological_process	medium-chain fatty acid metabolic process
H	0052689	molecular_function	carboxylic ester hydrolase activity
H	0070857	biological_process	regulation of bile acid biosynthetic process
H	0071397	biological_process	cellular response to cholesterol
H	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
H	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
H	0090205	biological_process	positive regulation of cholesterol metabolic process
H	0106435	molecular_function	carboxylesterase activity
H	0120188	biological_process	regulation of bile acid secretion
I	0004771	molecular_function	sterol esterase activity
I	0005737	cellular_component	cytoplasm
I	0005783	cellular_component	endoplasmic reticulum
I	0005788	cellular_component	endoplasmic reticulum lumen
I	0005811	cellular_component	lipid droplet
I	0005829	cellular_component	cytosol
I	0006629	biological_process	lipid metabolic process
I	0006695	biological_process	cholesterol biosynthetic process
I	0008203	biological_process	cholesterol metabolic process
I	0009636	biological_process	response to toxic substance
I	0010875	biological_process	positive regulation of cholesterol efflux
I	0010887	biological_process	negative regulation of cholesterol storage
I	0016042	biological_process	lipid catabolic process
I	0030855	biological_process	epithelial cell differentiation
I	0042632	biological_process	cholesterol homeostasis
I	0043691	biological_process	reverse cholesterol transport
I	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
I	0051791	biological_process	medium-chain fatty acid metabolic process
I	0052689	molecular_function	carboxylic ester hydrolase activity
I	0070857	biological_process	regulation of bile acid biosynthetic process
I	0071397	biological_process	cellular response to cholesterol
I	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
I	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
I	0090205	biological_process	positive regulation of cholesterol metabolic process
I	0106435	molecular_function	carboxylesterase activity
I	0120188	biological_process	regulation of bile acid secretion
J	0004771	molecular_function	sterol esterase activity
J	0005737	cellular_component	cytoplasm
J	0005783	cellular_component	endoplasmic reticulum
J	0005788	cellular_component	endoplasmic reticulum lumen
J	0005811	cellular_component	lipid droplet
J	0005829	cellular_component	cytosol
J	0006629	biological_process	lipid metabolic process
J	0006695	biological_process	cholesterol biosynthetic process
J	0008203	biological_process	cholesterol metabolic process
J	0009636	biological_process	response to toxic substance
J	0010875	biological_process	positive regulation of cholesterol efflux
J	0010887	biological_process	negative regulation of cholesterol storage
J	0016042	biological_process	lipid catabolic process
J	0030855	biological_process	epithelial cell differentiation
J	0042632	biological_process	cholesterol homeostasis
J	0043691	biological_process	reverse cholesterol transport
J	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
J	0051791	biological_process	medium-chain fatty acid metabolic process
J	0052689	molecular_function	carboxylic ester hydrolase activity
J	0070857	biological_process	regulation of bile acid biosynthetic process
J	0071397	biological_process	cellular response to cholesterol
J	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
J	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
J	0090205	biological_process	positive regulation of cholesterol metabolic process
J	0106435	molecular_function	carboxylesterase activity
J	0120188	biological_process	regulation of bile acid secretion
K	0004771	molecular_function	sterol esterase activity
K	0005737	cellular_component	cytoplasm
K	0005783	cellular_component	endoplasmic reticulum
K	0005788	cellular_component	endoplasmic reticulum lumen
K	0005811	cellular_component	lipid droplet
K	0005829	cellular_component	cytosol
K	0006629	biological_process	lipid metabolic process
K	0006695	biological_process	cholesterol biosynthetic process
K	0008203	biological_process	cholesterol metabolic process
K	0009636	biological_process	response to toxic substance
K	0010875	biological_process	positive regulation of cholesterol efflux
K	0010887	biological_process	negative regulation of cholesterol storage
K	0016042	biological_process	lipid catabolic process
K	0030855	biological_process	epithelial cell differentiation
K	0042632	biological_process	cholesterol homeostasis
K	0043691	biological_process	reverse cholesterol transport
K	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
K	0051791	biological_process	medium-chain fatty acid metabolic process
K	0052689	molecular_function	carboxylic ester hydrolase activity
K	0070857	biological_process	regulation of bile acid biosynthetic process
K	0071397	biological_process	cellular response to cholesterol
K	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
K	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
K	0090205	biological_process	positive regulation of cholesterol metabolic process
K	0106435	molecular_function	carboxylesterase activity
K	0120188	biological_process	regulation of bile acid secretion
L	0004771	molecular_function	sterol esterase activity
L	0005737	cellular_component	cytoplasm
L	0005783	cellular_component	endoplasmic reticulum
L	0005788	cellular_component	endoplasmic reticulum lumen
L	0005811	cellular_component	lipid droplet
L	0005829	cellular_component	cytosol
L	0006629	biological_process	lipid metabolic process
L	0006695	biological_process	cholesterol biosynthetic process
L	0008203	biological_process	cholesterol metabolic process
L	0009636	biological_process	response to toxic substance
L	0010875	biological_process	positive regulation of cholesterol efflux
L	0010887	biological_process	negative regulation of cholesterol storage
L	0016042	biological_process	lipid catabolic process
L	0030855	biological_process	epithelial cell differentiation
L	0042632	biological_process	cholesterol homeostasis
L	0043691	biological_process	reverse cholesterol transport
L	0047374	molecular_function	methylumbelliferyl-acetate deacetylase activity
L	0051791	biological_process	medium-chain fatty acid metabolic process
L	0052689	molecular_function	carboxylic ester hydrolase activity
L	0070857	biological_process	regulation of bile acid biosynthetic process
L	0071397	biological_process	cellular response to cholesterol
L	0071404	biological_process	cellular response to low-density lipoprotein particle stimulus
L	0090122	biological_process	cholesterol ester hydrolysis involved in cholesterol transport
L	0090205	biological_process	positive regulation of cholesterol metabolic process
L	0106435	molecular_function	carboxylesterase activity
L	0120188	biological_process	regulation of bile acid secretion

Functional Information from PROSITE/UniProt

site_id	PS00122
Number of Residues	16
Details	CARBOXYLESTERASE_B_1 Carboxylesterases type-B serine active site. FGGnpgsVtIfGeSAG

Chain	Residue	Details
A	PHE208-GLY223

---

site_id	PS00941
Number of Residues	11
Details	CARBOXYLESTERASE_B_2 Carboxylesterases type-B signature 2. EDCLYLNIYtP

Chain	Residue	Details
A	GLU114-PRO124

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Acyl-ester intermediate => ECO:0000255|PROSITE-ProRule:PRU10039, ECO:0000269|PubMed:12022871

Chain	Residue	Details
A	SER221
J	SER221
K	SER221
L	SER221
B	SER221
C	SER221
D	SER221
E	SER221
F	SER221
G	SER221
H	SER221
I	SER221

---

site_id	SWS_FT_FI2
Number of Residues	24
Details	ACT_SITE: Charge relay system => ECO:0000269|PubMed:12022871

Chain	Residue	Details
A	GLU354
E	GLY469
F	GLU354
F	GLY469
G	GLU354
G	GLY469
H	GLU354
H	GLY469
I	GLU354
I	GLY469
J	GLU354
A	GLY469
J	GLY469
K	GLU354
K	GLY469
L	GLU354
L	GLY469
B	GLU354
B	GLY469
C	GLU354
C	GLY469
D	GLU354
D	GLY469
E	GLU354

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	LEU381
J	LEU381
K	LEU381
L	LEU381
B	LEU381
C	LEU381
D	LEU381
E	LEU381
F	LEU381
G	LEU381
H	LEU381
I	LEU381

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12022871

Chain	Residue	Details
A	ASN79
J	ASN79
K	ASN79
L	ASN79
B	ASN79
C	ASN79
D	ASN79
E	ASN79
F	ASN79
G	ASN79
H	ASN79
I	ASN79

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
A	SER221
A	GLU354
A	HIS468

---

site_id	CSA10
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
J	SER221
J	GLU354
J	HIS468

---

site_id	CSA11
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
K	SER221
K	GLU354
K	HIS468

---

site_id	CSA12
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
L	SER221
L	GLU354
L	HIS468

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
B	SER221
B	GLU354
B	HIS468

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
C	SER221
C	GLU354
C	HIS468

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
D	SER221
D	GLU354
D	HIS468

---

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
E	SER221
E	GLU354
E	HIS468

---

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
F	SER221
F	GLU354
F	HIS468

---

site_id	CSA7
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
G	SER221
G	GLU354
G	HIS468

---

site_id	CSA8
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
H	SER221
H	GLU354
H	HIS468

---

site_id	CSA9
Number of Residues	3
Details	Annotated By Reference To The Literature 1qe3

Chain	Residue	Details
I	SER221
I	GLU354
I	HIS468

---