1Y9G

Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000272	biological_process	polysaccharide catabolic process
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0004575	molecular_function	sucrose alpha-glucosidase activity
A	0005576	cellular_component	extracellular region
A	0005737	cellular_component	cytoplasm
A	0005975	biological_process	carbohydrate metabolic process
A	0005987	biological_process	sucrose catabolic process
A	0008152	biological_process	metabolic process
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0051669	molecular_function	fructan beta-fructosidase activity

Functional Information from PROSITE/UniProt

site_id	PS00609
Number of Residues	14
Details	GLYCOSYL_HYDROL_F32 Glycosyl hydrolases family 32 active site. HfsPqknwMNDPNG

Chain	Residue	Details
A	HIS31-GLY44

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000255|PROSITE-ProRule:PRU10067

Chain	Residue	Details
A	ASP41

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor/acceptor => ECO:0000255|PROSITE-ProRule:PRU10067

Chain	Residue	Details
A	GLU241

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site_id	SWS_FT_FI3
Number of Residues	7
Details	BINDING:

Chain	Residue	Details
A	ASN40
A	GLN57
A	TRP65
A	SER103
A	ARG188
A	GLU241
A	TRP335

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site_id	SWS_FT_FI4
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN49
A	ASN112
A	ASN254

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site_id	SWS_FT_FI5
Number of Residues	5
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:15522299

Chain	Residue	Details
A	ASN67
A	ASN111
A	ASN300
A	ASN398
A	ASN430

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 827

Chain	Residue	Details
A	ASP41	covalently attached, nucleofuge, nucleophile
A	GLU241	activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor

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