1Y9G
Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.904, 94.371, 67.617 |
| Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
| Resolution | 24.050 - 1.870 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.19473 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.865 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.050 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.072 | 0.369 |
| Number of reflections | 41994 | |
| <I/σ(I)> | 10.1 | 2.2 |
| Completeness [%] | 84.9 | 75.58 |
| Redundancy | 5.4 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 15% PEG3350, 1 mM sodium cacodylate, 20-100 mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
