Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1Y8R

SUMO E1 ACTIVATING ENZYME SAE1-SAE2-SUMO1-MG-ATP COMPLEX

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004839	molecular_function	ubiquitin activating enzyme activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0008047	molecular_function	enzyme activator activity
A	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
A	0016567	biological_process	protein ubiquitination
A	0016874	molecular_function	ligase activity
A	0016925	biological_process	protein sumoylation
A	0019948	molecular_function	SUMO activating enzyme activity
A	0031510	cellular_component	SUMO activating enzyme complex
A	0032446	biological_process	protein modification by small protein conjugation
A	0033235	biological_process	positive regulation of protein sumoylation
A	0036211	biological_process	protein modification process
A	0043008	molecular_function	ATP-dependent protein binding
A	0044388	molecular_function	small protein activating enzyme binding
A	0046982	molecular_function	protein heterodimerization activity
A	1903955	biological_process	positive regulation of protein targeting to mitochondrion
B	0000287	molecular_function	magnesium ion binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
B	0016740	molecular_function	transferase activity
B	0016925	biological_process	protein sumoylation
B	0019948	molecular_function	SUMO activating enzyme activity
B	0031510	cellular_component	SUMO activating enzyme complex
B	0032183	molecular_function	SUMO binding
B	0033235	biological_process	positive regulation of protein sumoylation
B	0044388	molecular_function	small protein activating enzyme binding
B	0044390	molecular_function	ubiquitin-like protein conjugating enzyme binding
B	0046872	molecular_function	metal ion binding
B	0046982	molecular_function	protein heterodimerization activity
C	0001741	cellular_component	XY body
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005643	cellular_component	nuclear pore
C	0005654	cellular_component	nucleoplasm
C	0005730	cellular_component	nucleolus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006281	biological_process	DNA repair
C	0006355	biological_process	regulation of DNA-templated transcription
C	0008076	cellular_component	voltage-gated potassium channel complex
C	0008134	molecular_function	transcription factor binding
C	0010621	biological_process	negative regulation of transcription by transcription factor localization
C	0015459	molecular_function	potassium channel regulator activity
C	0016604	cellular_component	nuclear body
C	0016605	cellular_component	PML body
C	0016607	cellular_component	nuclear speck
C	0016925	biological_process	protein sumoylation
C	0019899	molecular_function	enzyme binding
C	0030578	biological_process	PML body organization
C	0031334	biological_process	positive regulation of protein-containing complex assembly
C	0031386	molecular_function	protein tag activity
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0031647	biological_process	regulation of protein stability
C	0031965	cellular_component	nuclear membrane
C	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
C	0032880	biological_process	regulation of protein localization
C	0034605	biological_process	cellular response to heat
C	0042308	biological_process	negative regulation of protein import into nucleus
C	0043392	biological_process	negative regulation of DNA binding
C	0043433	biological_process	negative regulation of DNA-binding transcription factor activity
C	0044388	molecular_function	small protein activating enzyme binding
C	0044389	molecular_function	ubiquitin-like protein ligase binding
C	0045759	biological_process	negative regulation of action potential
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0050821	biological_process	protein stabilization
C	0060021	biological_process	roof of mouth development
C	0071276	biological_process	cellular response to cadmium ion
C	0086004	biological_process	regulation of cardiac muscle cell contraction
C	0090204	biological_process	protein localization to nuclear pore
C	0097165	cellular_component	nuclear stress granule
C	0098978	cellular_component	glutamatergic synapse
C	0099523	cellular_component	presynaptic cytosol
C	0099524	cellular_component	postsynaptic cytosol
C	1901896	biological_process	positive regulation of ATPase-coupled calcium transmembrane transporter activity
C	1902260	biological_process	negative regulation of delayed rectifier potassium channel activity
C	1903169	biological_process	regulation of calcium ion transmembrane transport
C	1990381	molecular_function	ubiquitin-specific protease binding
D	0004839	molecular_function	ubiquitin activating enzyme activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0008047	molecular_function	enzyme activator activity
D	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
D	0016567	biological_process	protein ubiquitination
D	0016874	molecular_function	ligase activity
D	0016925	biological_process	protein sumoylation
D	0019948	molecular_function	SUMO activating enzyme activity
D	0031510	cellular_component	SUMO activating enzyme complex
D	0032446	biological_process	protein modification by small protein conjugation
D	0033235	biological_process	positive regulation of protein sumoylation
D	0036211	biological_process	protein modification process
D	0043008	molecular_function	ATP-dependent protein binding
D	0044388	molecular_function	small protein activating enzyme binding
D	0046982	molecular_function	protein heterodimerization activity
D	1903955	biological_process	positive regulation of protein targeting to mitochondrion
E	0000287	molecular_function	magnesium ion binding
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
E	0016740	molecular_function	transferase activity
E	0016925	biological_process	protein sumoylation
E	0019948	molecular_function	SUMO activating enzyme activity
E	0031510	cellular_component	SUMO activating enzyme complex
E	0032183	molecular_function	SUMO binding
E	0033235	biological_process	positive regulation of protein sumoylation
E	0044388	molecular_function	small protein activating enzyme binding
E	0044390	molecular_function	ubiquitin-like protein conjugating enzyme binding
E	0046872	molecular_function	metal ion binding
E	0046982	molecular_function	protein heterodimerization activity
F	0001741	cellular_component	XY body
F	0003723	molecular_function	RNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005643	cellular_component	nuclear pore
F	0005654	cellular_component	nucleoplasm
F	0005730	cellular_component	nucleolus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006281	biological_process	DNA repair
F	0006355	biological_process	regulation of DNA-templated transcription
F	0008076	cellular_component	voltage-gated potassium channel complex
F	0008134	molecular_function	transcription factor binding
F	0010621	biological_process	negative regulation of transcription by transcription factor localization
F	0015459	molecular_function	potassium channel regulator activity
F	0016604	cellular_component	nuclear body
F	0016605	cellular_component	PML body
F	0016607	cellular_component	nuclear speck
F	0016925	biological_process	protein sumoylation
F	0019899	molecular_function	enzyme binding
F	0030578	biological_process	PML body organization
F	0031334	biological_process	positive regulation of protein-containing complex assembly
F	0031386	molecular_function	protein tag activity
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0031647	biological_process	regulation of protein stability
F	0031965	cellular_component	nuclear membrane
F	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
F	0032880	biological_process	regulation of protein localization
F	0034605	biological_process	cellular response to heat
F	0042308	biological_process	negative regulation of protein import into nucleus
F	0043392	biological_process	negative regulation of DNA binding
F	0043433	biological_process	negative regulation of DNA-binding transcription factor activity
F	0044388	molecular_function	small protein activating enzyme binding
F	0044389	molecular_function	ubiquitin-like protein ligase binding
F	0045759	biological_process	negative regulation of action potential
F	0045892	biological_process	negative regulation of DNA-templated transcription
F	0050821	biological_process	protein stabilization
F	0060021	biological_process	roof of mouth development
F	0071276	biological_process	cellular response to cadmium ion
F	0086004	biological_process	regulation of cardiac muscle cell contraction
F	0090204	biological_process	protein localization to nuclear pore
F	0097165	cellular_component	nuclear stress granule
F	0098978	cellular_component	glutamatergic synapse
F	0099523	cellular_component	presynaptic cytosol
F	0099524	cellular_component	postsynaptic cytosol
F	1901896	biological_process	positive regulation of ATPase-coupled calcium transmembrane transporter activity
F	1902260	biological_process	negative regulation of delayed rectifier potassium channel activity
F	1903169	biological_process	regulation of calcium ion transmembrane transport
F	1990381	molecular_function	ubiquitin-specific protease binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG E 641

Chain	Residue
E	ASP117
E	ATP801
E	HOH852
E	HOH853

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 641

Chain	Residue
B	ASP117
B	ATP802
B	HOH803
B	HOH804

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 642

Chain	Residue
E	CYS161
E	CYS441
E	CYS444
E	CYS158

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 642

Chain	Residue
B	CYS158
B	CYS161
B	CYS441
B	CYS444

site_id	AC5
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ATP E 801

Chain	Residue
D	ARG21
E	ALA25
E	GLY26
E	GLY27
E	ASP48
E	LEU49
E	ASP50
E	ASN56
E	ARG59
E	GLN60
E	LYS72
E	SER95
E	ILE96
E	ASP117
E	ALA121
E	LYS346
E	MG641
E	HOH817
E	HOH827
E	HOH852
E	HOH853
F	GLY97

site_id	AC6
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ATP B 802

Chain	Residue
A	ARG21
B	GLY26
B	GLY27
B	ASP48
B	LEU49
B	ASP50
B	ASN56
B	ARG59
B	GLN60
B	LYS72
B	SER95
B	ILE96
B	ASP117
B	ASN118
B	MG641
B	HOH803
B	HOH804
B	HOH852
B	HOH854
C	GLY97

Functional Information from PROSITE/UniProt

site_id	PS00536
Number of Residues	9
Details	UBIQUITIN_ACTIVAT_1 Ubiquitin-activating enzyme signature 1. KILSGKIdQ

Chain	Residue	Details
B	LYS404-GLN412

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	SITE: Interaction with PIAS2

Chain	Residue	Details
C	PHE36
F	PHE36

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:18707152, ECO:0007744\|PubMed:17081983, ECO:0007744\|PubMed:18691976, ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:21406692, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
C	SER2
E	LYS72
E	SER95
E	ASP117
F	SER2
B	ASN56
B	LYS72
B	SER95
B	ASP117
E	GLY24
E	ASP48
E	ASN56

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231

Chain	Residue	Details
C	SER9
F	SER9
B	CYS441
B	CYS444
E	CYS158
E	CYS161
E	CYS441
E	CYS444

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
C	SER32
F	SER32

site_id	SWS_FT_FI5
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744\|PubMed:25218447, ECO:0007744\|PubMed:25755297, ECO:0007744\|PubMed:25772364, ECO:0007744\|PubMed:28112733

Chain	Residue	Details
C	LYS7
F	LYS7

site_id	SWS_FT_FI6
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) => ECO:0000269\|PubMed:27068747

Chain	Residue	Details
B	SER507
C	GLY97
F	GLY97

site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:25218447, ECO:0007744\|PubMed:28112733

Chain	Residue	Details
C	LYS16
F	LYS16

site_id	SWS_FT_FI8
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:25218447, ECO:0007744\|PubMed:25772364, ECO:0007744\|PubMed:28112733

Chain	Residue	Details
C	LYS17
F	LYS17

site_id	SWS_FT_FI9
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
C	LYS23
C	LYS39
C	LYS45
C	LYS46
F	LYS23
F	LYS39
F	LYS45
F	LYS46

site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)

Chain	Residue	Details
C	LYS25
E	LYS611
E	LYS617
E	LYS623
F	LYS25
B	LYS275
B	LYS611
B	LYS617
B	LYS623
E	LYS190
E	LYS275

site_id	SWS_FT_FI11
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:25755297, ECO:0007744\|PubMed:28112733

Chain	Residue	Details
C	LYS37
F	LYS37

site_id	SWS_FT_FI12
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
B	LYS257
B	LYS420
E	LYS257
E	LYS420

site_id	SWS_FT_FI13
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate

Chain	Residue	Details
B	LYS271
B	LYS613
E	LYS271
E	LYS613

site_id	SWS_FT_FI14
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
B	LYS371
B	LYS540
E	LYS371
E	LYS540

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)