1Y8R

SUMO E1 ACTIVATING ENZYME SAE1-SAE2-SUMO1-MG-ATP COMPLEX

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004839	molecular_function	ubiquitin activating enzyme activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008047	molecular_function	enzyme activator activity
A	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
A	0016567	biological_process	protein ubiquitination
A	0016874	molecular_function	ligase activity
A	0016925	biological_process	protein sumoylation
A	0019948	molecular_function	SUMO activating enzyme activity
A	0031510	cellular_component	SUMO activating enzyme complex
A	0033235	biological_process	positive regulation of protein sumoylation
A	0036211	biological_process	protein modification process
A	0043008	molecular_function	ATP-dependent protein binding
A	0044388	molecular_function	small protein activating enzyme binding
A	0046982	molecular_function	protein heterodimerization activity
A	1903955	biological_process	positive regulation of protein targeting to mitochondrion
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
B	0016740	molecular_function	transferase activity
B	0016925	biological_process	protein sumoylation
B	0019948	molecular_function	SUMO activating enzyme activity
B	0031510	cellular_component	SUMO activating enzyme complex
B	0032183	molecular_function	SUMO binding
B	0033235	biological_process	positive regulation of protein sumoylation
B	0044388	molecular_function	small protein activating enzyme binding
B	0044390	molecular_function	ubiquitin-like protein conjugating enzyme binding
B	0046872	molecular_function	metal ion binding
B	0046982	molecular_function	protein heterodimerization activity
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0001741	cellular_component	XY body
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005643	cellular_component	nuclear pore
C	0005654	cellular_component	nucleoplasm
C	0005730	cellular_component	nucleolus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006281	biological_process	DNA repair
C	0015459	molecular_function	potassium channel regulator activity
C	0016604	cellular_component	nuclear body
C	0016605	cellular_component	PML body
C	0016607	cellular_component	nuclear speck
C	0016925	biological_process	protein sumoylation
C	0019899	molecular_function	enzyme binding
C	0031334	biological_process	positive regulation of protein-containing complex assembly
C	0031386	molecular_function	protein tag activity
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0031647	biological_process	regulation of protein stability
C	0031965	cellular_component	nuclear membrane
C	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
C	0034605	biological_process	cellular response to heat
C	0042308	biological_process	negative regulation of protein import into nucleus
C	0044388	molecular_function	small protein activating enzyme binding
C	0044389	molecular_function	ubiquitin-like protein ligase binding
C	0045759	biological_process	negative regulation of action potential
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0050821	biological_process	protein stabilization
C	0060021	biological_process	roof of mouth development
C	0060633	biological_process	negative regulation of transcription initiation by RNA polymerase II
C	0071276	biological_process	cellular response to cadmium ion
C	0086004	biological_process	regulation of cardiac muscle cell contraction
C	0097165	cellular_component	nuclear stress granule
C	0098978	cellular_component	glutamatergic synapse
C	0099523	cellular_component	presynaptic cytosol
C	0099524	cellular_component	postsynaptic cytosol
C	0141109	molecular_function	transporter activator activity
C	1903169	biological_process	regulation of calcium ion transmembrane transport
C	1990381	molecular_function	ubiquitin-specific protease binding
D	0004839	molecular_function	ubiquitin activating enzyme activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0008047	molecular_function	enzyme activator activity
D	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
D	0016567	biological_process	protein ubiquitination
D	0016874	molecular_function	ligase activity
D	0016925	biological_process	protein sumoylation
D	0019948	molecular_function	SUMO activating enzyme activity
D	0031510	cellular_component	SUMO activating enzyme complex
D	0033235	biological_process	positive regulation of protein sumoylation
D	0036211	biological_process	protein modification process
D	0043008	molecular_function	ATP-dependent protein binding
D	0044388	molecular_function	small protein activating enzyme binding
D	0046982	molecular_function	protein heterodimerization activity
D	1903955	biological_process	positive regulation of protein targeting to mitochondrion
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
E	0016740	molecular_function	transferase activity
E	0016925	biological_process	protein sumoylation
E	0019948	molecular_function	SUMO activating enzyme activity
E	0031510	cellular_component	SUMO activating enzyme complex
E	0032183	molecular_function	SUMO binding
E	0033235	biological_process	positive regulation of protein sumoylation
E	0044388	molecular_function	small protein activating enzyme binding
E	0044390	molecular_function	ubiquitin-like protein conjugating enzyme binding
E	0046872	molecular_function	metal ion binding
E	0046982	molecular_function	protein heterodimerization activity
F	0000122	biological_process	negative regulation of transcription by RNA polymerase II
F	0001741	cellular_component	XY body
F	0003723	molecular_function	RNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005643	cellular_component	nuclear pore
F	0005654	cellular_component	nucleoplasm
F	0005730	cellular_component	nucleolus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006281	biological_process	DNA repair
F	0015459	molecular_function	potassium channel regulator activity
F	0016604	cellular_component	nuclear body
F	0016605	cellular_component	PML body
F	0016607	cellular_component	nuclear speck
F	0016925	biological_process	protein sumoylation
F	0019899	molecular_function	enzyme binding
F	0031334	biological_process	positive regulation of protein-containing complex assembly
F	0031386	molecular_function	protein tag activity
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0031647	biological_process	regulation of protein stability
F	0031965	cellular_component	nuclear membrane
F	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
F	0034605	biological_process	cellular response to heat
F	0042308	biological_process	negative regulation of protein import into nucleus
F	0044388	molecular_function	small protein activating enzyme binding
F	0044389	molecular_function	ubiquitin-like protein ligase binding
F	0045759	biological_process	negative regulation of action potential
F	0045892	biological_process	negative regulation of DNA-templated transcription
F	0050821	biological_process	protein stabilization
F	0060021	biological_process	roof of mouth development
F	0060633	biological_process	negative regulation of transcription initiation by RNA polymerase II
F	0071276	biological_process	cellular response to cadmium ion
F	0086004	biological_process	regulation of cardiac muscle cell contraction
F	0097165	cellular_component	nuclear stress granule
F	0098978	cellular_component	glutamatergic synapse
F	0099523	cellular_component	presynaptic cytosol
F	0099524	cellular_component	postsynaptic cytosol
F	0141109	molecular_function	transporter activator activity
F	1903169	biological_process	regulation of calcium ion transmembrane transport
F	1990381	molecular_function	ubiquitin-specific protease binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG E 641

Chain	Residue
E	ASP117
E	ATP801
E	HOH852
E	HOH853

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 641

Chain	Residue
B	ASP117
B	ATP802
B	HOH803
B	HOH804

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 642

Chain	Residue
E	CYS161
E	CYS441
E	CYS444
E	CYS158

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 642

Chain	Residue
B	CYS158
B	CYS161
B	CYS441
B	CYS444

---

site_id	AC5
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ATP E 801

Chain	Residue
D	ARG21
E	ALA25
E	GLY26
E	GLY27
E	ASP48
E	LEU49
E	ASP50
E	ASN56
E	ARG59
E	GLN60
E	LYS72
E	SER95
E	ILE96
E	ASP117
E	ALA121
E	LYS346
E	MG641
E	HOH817
E	HOH827
E	HOH852
E	HOH853
F	GLY97

---

site_id	AC6
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ATP B 802

Chain	Residue
A	ARG21
B	GLY26
B	GLY27
B	ASP48
B	LEU49
B	ASP50
B	ASN56
B	ARG59
B	GLN60
B	LYS72
B	SER95
B	ILE96
B	ASP117
B	ASN118
B	MG641
B	HOH803
B	HOH804
B	HOH852
B	HOH854
C	GLY97

---

Functional Information from PROSITE/UniProt

site_id	PS00536
Number of Residues	9
Details	UBIQUITIN_ACTIVAT_1 Ubiquitin-activating enzyme signature 1. KILSGKIdQ

Chain	Residue	Details
B	LYS404-GLN412

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU10132","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15660128","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20164921","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15660128","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)","evidences":[{"source":"PubMed","id":"25114211","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	14
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate"}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	154
Details	Domain: {"description":"Ubiquitin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	24
Details	Region: {"description":"(Microbial infection) Interaction with Tula hantavirus","evidences":[{"source":"PubMed","id":"12606074","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	Site: {"description":"Interaction with PIAS2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25218447","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)","evidences":[{"source":"PubMed","id":"27068747","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25218447","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25772364","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25755297","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---