1XO1

T5 5'-EXONUCLEASE MUTANT K83A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003824	molecular_function	catalytic activity
A	0004519	molecular_function	endonuclease activity
A	0004527	molecular_function	exonuclease activity
A	0004529	molecular_function	DNA exonuclease activity
A	0006260	biological_process	DNA replication
A	0008409	molecular_function	5'-3' exonuclease activity
A	0016788	molecular_function	hydrolase activity, acting on ester bonds
A	0017108	molecular_function	5'-flap endonuclease activity
A	0019034	cellular_component	viral replication complex
A	0019086	biological_process	late viral transcription
A	0033567	biological_process	DNA replication, Okazaki fragment processing
A	0035312	molecular_function	5'-3' DNA exonuclease activity
A	0039693	biological_process	viral DNA genome replication
A	0046872	molecular_function	metal ion binding
A	0048256	molecular_function	flap endonuclease activity
A	0051908	molecular_function	double-stranded DNA 5'-3' DNA exonuclease activity
A	0140640	molecular_function	catalytic activity, acting on a nucleic acid
A	1990238	molecular_function	double-stranded DNA endonuclease activity
B	0003677	molecular_function	DNA binding
B	0003824	molecular_function	catalytic activity
B	0004519	molecular_function	endonuclease activity
B	0004527	molecular_function	exonuclease activity
B	0004529	molecular_function	DNA exonuclease activity
B	0006260	biological_process	DNA replication
B	0008409	molecular_function	5'-3' exonuclease activity
B	0016788	molecular_function	hydrolase activity, acting on ester bonds
B	0017108	molecular_function	5'-flap endonuclease activity
B	0019034	cellular_component	viral replication complex
B	0019086	biological_process	late viral transcription
B	0033567	biological_process	DNA replication, Okazaki fragment processing
B	0035312	molecular_function	5'-3' DNA exonuclease activity
B	0039693	biological_process	viral DNA genome replication
B	0046872	molecular_function	metal ion binding
B	0048256	molecular_function	flap endonuclease activity
B	0051908	molecular_function	double-stranded DNA 5'-3' DNA exonuclease activity
B	0140640	molecular_function	catalytic activity, acting on a nucleic acid
B	1990238	molecular_function	double-stranded DNA endonuclease activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_04140, ECO:0000269|PubMed:10364212

Chain	Residue	Details
A	ALA83
B	ALA83

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site_id	SWS_FT_FI2
Number of Residues	10
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_04140, ECO:0000269|PubMed:27273516

Chain	Residue	Details
A	ASP201
A	VAL209
A	ILE212
B	ASP130
B	ASP155
B	ASP201
B	VAL209
B	ILE212
A	ASP130
A	ASP155

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site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_04140, ECO:0000269|PubMed:15077103, ECO:0000269|PubMed:27273516, ECO:0000269|PubMed:8657312

Chain	Residue	Details
A	ASP153
B	ASP153

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	10
Details	M-CSA 549

Chain	Residue	Details
A	ASP26	metal ligand
A	ASP68	metal ligand
A	ALA83	electrostatic stabiliser, proton shuttle (general acid/base)
A	ARG86	electrostatic stabiliser
A	GLU128	metal ligand
A	ASP131	metal ligand
A	ASP153	metal ligand
A	ASP155	metal ligand
A	ASP201	metal ligand
A	ASP204	metal ligand

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site_id	MCSA2
Number of Residues	10
Details	M-CSA 549

Chain	Residue	Details
B	ASP26	metal ligand
B	ASP68	metal ligand
B	ALA83	electrostatic stabiliser, proton shuttle (general acid/base)
B	ARG86	electrostatic stabiliser
B	GLU128	metal ligand
B	ASP131	metal ligand
B	ASP153	metal ligand
B	ASP155	metal ligand
B	ASP201	metal ligand
B	ASP204	metal ligand

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