1XO1

T5 5'-EXONUCLEASE MUTANT K83A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Detector technology	AREA DETECTOR
Detector	XENTRONICS
Spacegroup name	P 21 21 21
Unit cell lengths	66.890, 76.810, 120.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.226 *
Rwork	0.226
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1exn
RMSD bond length	0.006
RMSD bond angle	0.960 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.500
Rmerge	0.082 *
Total number of observations	158564 *
Number of reflections	21915
Completeness [%]	99.6
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		Ceska, T.A., (1993) J.Mol.Biol., 233, 179. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	sodium citrate	100 (mM)	pH5.6
3	1	reservoir	ammonium sulfate	35 (%(w/v))
4	1	drop	potassium phosphate	25 (mM)	pH7.5
5	1	drop	EDTA	1 (mM)
6	1	drop	dithiothreitol	1 (mM)
7	1	drop		100 (mM)
8	1	drop	glycerol	5 (%(v/v))