Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1XLT

Crystal structure of Transhydrogenase [(domain I)2:domain III] heterotrimer complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0006740	biological_process	NADPH regeneration
A	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
A	0016491	molecular_function	oxidoreductase activity
A	0046983	molecular_function	protein dimerization activity
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
A	0070403	molecular_function	NAD+ binding
A	0070404	molecular_function	NADH binding
A	1902600	biological_process	proton transmembrane transport
B	0000166	molecular_function	nucleotide binding
B	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0006740	biological_process	NADPH regeneration
B	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
B	0016491	molecular_function	oxidoreductase activity
B	0046983	molecular_function	protein dimerization activity
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
B	0070403	molecular_function	NAD+ binding
B	0070404	molecular_function	NADH binding
B	1902600	biological_process	proton transmembrane transport
D	0000166	molecular_function	nucleotide binding
D	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
D	0005515	molecular_function	protein binding
D	0005886	cellular_component	plasma membrane
D	0006740	biological_process	NADPH regeneration
D	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
D	0016491	molecular_function	oxidoreductase activity
D	0046983	molecular_function	protein dimerization activity
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
D	0070403	molecular_function	NAD+ binding
D	0070404	molecular_function	NADH binding
D	1902600	biological_process	proton transmembrane transport
E	0000166	molecular_function	nucleotide binding
E	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
E	0005515	molecular_function	protein binding
E	0005886	cellular_component	plasma membrane
E	0006740	biological_process	NADPH regeneration
E	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
E	0016491	molecular_function	oxidoreductase activity
E	0046983	molecular_function	protein dimerization activity
E	0050661	molecular_function	NADP binding
E	0051287	molecular_function	NAD binding
E	0070403	molecular_function	NAD+ binding
E	0070404	molecular_function	NADH binding
E	1902600	biological_process	proton transmembrane transport
G	0000166	molecular_function	nucleotide binding
G	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
G	0005515	molecular_function	protein binding
G	0005886	cellular_component	plasma membrane
G	0006740	biological_process	NADPH regeneration
G	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
G	0016491	molecular_function	oxidoreductase activity
G	0046983	molecular_function	protein dimerization activity
G	0050661	molecular_function	NADP binding
G	0051287	molecular_function	NAD binding
G	0070403	molecular_function	NAD+ binding
G	0070404	molecular_function	NADH binding
G	1902600	biological_process	proton transmembrane transport
H	0000166	molecular_function	nucleotide binding
H	0003957	molecular_function	NAD(P)+ transhydrogenase (Si-specific) activity
H	0005515	molecular_function	protein binding
H	0005886	cellular_component	plasma membrane
H	0006740	biological_process	NADPH regeneration
H	0008750	molecular_function	proton-translocating NAD(P)+ transhydrogenase activity
H	0016491	molecular_function	oxidoreductase activity
H	0046983	molecular_function	protein dimerization activity
H	0050661	molecular_function	NADP binding
H	0051287	molecular_function	NAD binding
H	0070403	molecular_function	NAD+ binding
H	0070404	molecular_function	NADH binding
H	1902600	biological_process	proton transmembrane transport

Functional Information from PROSITE/UniProt

site_id	PS00836
Number of Residues	27
Details	ALADH_PNT_1 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1. AIPkErrpGEd..RVAiSPeVVkkLvGlG

Chain	Residue	Details
A	ALA4-GLY30

site_id	PS00837
Number of Residues	26
Details	ALADH_PNT_2 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 2. VFGVGvAGlqAiatAkRLGAvVmatD

Chain	Residue	Details
A	VAL177-ASP202

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	60
Details	Region: {"description":"RQD loop; involved in interaction with PntB"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	67
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10997900","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11250201","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15670609","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	54
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12791694","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15323555","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1PTJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1U2G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1djl

Chain	Residue	Details
C	TYR316
C	TYR432
C	ARG351

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1djl

Chain	Residue	Details
F	TYR316
F	TYR432
F	ARG351

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1djl

Chain	Residue	Details
I	TYR316
I	TYR432
I	ARG351

site_id	MCSA1
Number of Residues	1
Details	M-CSA 116

Chain	Residue	Details
C	ASP393	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	GLN132	steric locator
A	ASP135	hydrogen bond acceptor, steric role
A	SER138	electrostatic stabiliser

site_id	MCSA2
Number of Residues	1
Details	M-CSA 116

Chain	Residue	Details
F	ASP393	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	GLN132	steric locator
B	ASP135	hydrogen bond acceptor, steric role
B	SER138	electrostatic stabiliser

site_id	MCSA3
Number of Residues	1
Details	M-CSA 116

Chain	Residue	Details
I	ASP393	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
D	GLN132	steric locator
D	ASP135	hydrogen bond acceptor, steric role
D	SER138	electrostatic stabiliser
D	TYR235	polar/non-polar interaction, steric role

site_id	MCSA4
Number of Residues	4
Details	M-CSA 116

Chain	Residue	Details
E	ARG127	hydrogen bond donor, steric role
E	GLN132	steric locator
E	ASP135	hydrogen bond acceptor, steric role
E	SER138	electrostatic stabiliser

site_id	MCSA5
Number of Residues	4
Details	M-CSA 116

Chain	Residue	Details
G	ARG127	hydrogen bond donor, steric role
G	GLN132	steric locator
G	ASP135	hydrogen bond acceptor, steric role
G	SER138	electrostatic stabiliser

site_id	MCSA6
Number of Residues	4
Details	M-CSA 116

Chain	Residue	Details
H	ARG127	hydrogen bond donor, steric role
H	GLN132	steric locator
H	ASP135	hydrogen bond acceptor, steric role
H	SER138	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)