1XLT
Crystal structure of Transhydrogenase [(domain I)2:domain III] heterotrimer complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.372, 171.082, 203.181 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.680 - 3.100 |
| Rwork | 0.232 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | BEAST |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 58965 | |
| <I/σ(I)> | 12.9 | 1.9 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | Sodium Tartrate, PEG 3400, Sucrose, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
