1XLT

Crystal structure of Transhydrogenase [(domain I)2:domain III] heterotrimer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	95.372, 171.082, 203.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.680 - 3.100
Rwork	0.232
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.800
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	BEAST
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.000
High resolution limit [Å]	3.100	3.100
Number of reflections	58965
<I/σ(I)>	12.9	1.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	Sodium Tartrate, PEG 3400, Sucrose, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K