1XLM
D254E, D256E MUTANT OF D-XYLOSE ISOMERASE COMPLEXED WITH AL3 AND XYLITOL
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0009045 | molecular_function | xylose isomerase activity |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0042732 | biological_process | D-xylose metabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0009045 | molecular_function | xylose isomerase activity |
| B | 0016853 | molecular_function | isomerase activity |
| B | 0042732 | biological_process | D-xylose metabolic process |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | CTA |
| Number of Residues | 13 |
| Details | CATALYTIC SITE. |
| Chain | Residue |
| B | THR16 |
| A | LEU245 |
| A | GLN255 |
| A | LEU257 |
| A | GLY287 |
| B | ASP54 |
| B | LEU57 |
| B | SER94 |
| B | GLY137 |
| B | PRO181 |
| B | PRO183 |
| B | PRO186 |
| A | GLU220 |
| site_id | CTB |
| Number of Residues | 13 |
| Details | CATALYTIC SITE. |
| Chain | Residue |
| B | THR16 |
| B | ASP54 |
| B | LEU57 |
| B | SER94 |
| B | GLY137 |
| B | PRO181 |
| B | PRO183 |
| B | PRO186 |
| B | GLU220 |
| B | LEU245 |
| B | GLN255 |
| B | LEU257 |
| B | GLY287 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"2319597","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 14 |
| Details | Binding site: {} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1qum |
| Chain | Residue | Details |
| A | ARG297 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1qum |
| Chain | Residue | Details |
| B | ARG297 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1qum |
| Chain | Residue | Details |
| A | GLU180 | |
| A | LYS182 | |
| A | ASP56 | |
| A | HIS53 |
| site_id | CSA4 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1qum |
| Chain | Residue | Details |
| B | GLU180 | |
| B | LYS182 | |
| B | ASP56 | |
| B | HIS53 |
| site_id | MCSA1 |
| Number of Residues | 11 |
| Details | M-CSA 308 |
| Chain | Residue | Details |
| A | HIS53 | activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | GLU256 | metal ligand |
| A | ASP292 | activator, attractive charge-charge interaction, hydrogen bond acceptor, metal ligand, proton acceptor, proton donor |
| A | ASP56 | activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | MET87 | polar interaction, steric role |
| A | GLU180 | metal ligand |
| A | LYS182 | electrostatic stabiliser, hydrogen bond donor, proton donor |
| A | GLU216 | metal ligand |
| A | HIS219 | metal ligand |
| A | ASP244 | metal ligand |
| A | GLU254 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 11 |
| Details | M-CSA 308 |
| Chain | Residue | Details |
| B | HIS53 | activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | GLU256 | metal ligand |
| B | ASP292 | activator, attractive charge-charge interaction, hydrogen bond acceptor, metal ligand, proton acceptor, proton donor |
| B | ASP56 | activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | MET87 | polar interaction, steric role |
| B | GLU180 | metal ligand |
| B | LYS182 | electrostatic stabiliser, hydrogen bond donor, proton donor |
| B | GLU216 | metal ligand |
| B | HIS219 | metal ligand |
| B | ASP244 | metal ligand |
| B | GLU254 | metal ligand |
