1XLM
D254E, D256E MUTANT OF D-XYLOSE ISOMERASE COMPLEXED WITH AL3 AND XYLITOL
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-03 |
Detector | RIGAKU |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 139.600, 140.700, 83.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.1792 |
Rwork | 0.179 |
R-free | 0.22230 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xla |
RMSD bond length | 0.014 |
RMSD bond angle | 21.300 * |
Data reduction software | bioteX |
Data scaling software | bioteX |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 2.480 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.119 * | 0.520 |
Total number of observations | 142427 * | |
Number of reflections | 32570 | |
<I/σ(I)> | 4.87 | 1.1 |
Completeness [%] | 97.0 | 98 |
Redundancy | 4.4 | 2.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | PROTEIN WAS CRYSTALLIZED FROM 0.75 M (NH4)2SO4, 1.50 MM THYMOL, 0.05 TRIS, PH 7.0, THEN SOAKED INTO A SOLUTION CONTAINING 5 MM AL3+, 1.5 M XYLITOL, 1.50 M (NH4)2SO4 AND 6.0 M THYMOL IN 1.50 M TRIS-HCL PH 8.0 FOR TWO DAYS |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10.0 (mg/ml) | |
2 | 1 | drop | Tris | 0.05 (M) | |
3 | 1 | drop | ammonium sulfate | 0.75 (M) | |
4 | 1 | drop | thymol | 1.50 (mM) |