1XDS
Crystal structure of Aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM) and 11-deoxy-beta-rhodomycin (DbrA)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0008171 | molecular_function | O-methyltransferase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016831 | molecular_function | carboxy-lyase activity |
| A | 0017000 | biological_process | antibiotic biosynthetic process |
| A | 0032259 | biological_process | methylation |
| A | 0046983 | molecular_function | protein dimerization activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0008171 | molecular_function | O-methyltransferase activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016831 | molecular_function | carboxy-lyase activity |
| B | 0017000 | biological_process | antibiotic biosynthetic process |
| B | 0032259 | biological_process | methylation |
| B | 0046983 | molecular_function | protein dimerization activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE SAM A 5635 |
| Chain | Residue |
| A | TRP146 |
| A | SER255 |
| A | PHE256 |
| A | ASN260 |
| A | DRA5600 |
| A | HOH5640 |
| A | HOH5649 |
| A | HOH5654 |
| A | HOH5674 |
| A | HOH5688 |
| A | GLY190 |
| A | GLY191 |
| A | GLU213 |
| A | LEU214 |
| A | PRO217 |
| A | GLY239 |
| A | ASP240 |
| A | PHE241 |
| site_id | AC2 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE SAM B 9635 |
| Chain | Residue |
| B | GLY190 |
| B | GLY191 |
| B | GLU213 |
| B | PRO217 |
| B | ASP240 |
| B | PHE241 |
| B | SER255 |
| B | PHE256 |
| B | ASN260 |
| B | TRP261 |
| B | DRA9600 |
| B | HOH9644 |
| B | HOH9647 |
| B | HOH9671 |
| B | HOH9690 |
| site_id | AC3 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE DRA A 5600 |
| Chain | Residue |
| A | TRP109 |
| A | VAL116 |
| A | PHE145 |
| A | PHE159 |
| A | MSE163 |
| A | MSE196 |
| A | PHE256 |
| A | ASN260 |
| A | LEU304 |
| A | ARG307 |
| A | MSE308 |
| A | MSE312 |
| A | PHE346 |
| A | SAM5635 |
| site_id | AC4 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE DRA B 9600 |
| Chain | Residue |
| B | TRP109 |
| B | VAL116 |
| B | PHE145 |
| B | PHE159 |
| B | MSE163 |
| B | ALA173 |
| B | PRO174 |
| B | PHE256 |
| B | ASN260 |
| B | MSE308 |
| B | PHE311 |
| B | MSE312 |
| B | LEU344 |
| B | PHE346 |
| B | PHE348 |
| B | SAM9635 |
| B | HOH9688 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"14607118","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1QZZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1R00","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 10 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"14607118","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15548527","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1QZZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1R00","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1XDS","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Site: {"description":"Required for 4-O-methylation activity","evidences":[{"source":"PubMed","id":"39308748","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Site: {"description":"Required for 10-decarboxylative hydroxylation activity","evidences":[{"source":"PubMed","id":"39308748","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1kyw |
| Chain | Residue | Details |
| A | LEU259 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1kyw |
| Chain | Residue | Details |
| B | LEU259 |
