1XDS
Crystal structure of Aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM) and 11-deoxy-beta-rhodomycin (DbrA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.890, 79.890, 232.598 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.000 - 2.300 |
| R-factor | 0.25324 |
| Rwork | 0.251 |
| R-free | 0.29613 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RdmB+SAM complex |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.496 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 2.410 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 38051 | |
| <I/σ(I)> | 14.4 | 5.3 |
| Completeness [%] | 99.4 | 73.7 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | cobalt chloride, ammonium sulphate, MES-buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
