1XA8
Crystal Structure Analysis of Glutathione-dependent formaldehyde-activating enzyme (Gfa)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016846 | molecular_function | carbon-sulfur lyase activity |
| A | 0046294 | biological_process | formaldehyde catabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051907 | molecular_function | S-(hydroxymethyl)glutathione synthase activity |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016846 | molecular_function | carbon-sulfur lyase activity |
| B | 0046294 | biological_process | formaldehyde catabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051907 | molecular_function | S-(hydroxymethyl)glutathione synthase activity |
| C | 0008270 | molecular_function | zinc ion binding |
| C | 0016829 | molecular_function | lyase activity |
| C | 0016846 | molecular_function | carbon-sulfur lyase activity |
| C | 0046294 | biological_process | formaldehyde catabolic process |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051907 | molecular_function | S-(hydroxymethyl)glutathione synthase activity |
| D | 0008270 | molecular_function | zinc ion binding |
| D | 0016829 | molecular_function | lyase activity |
| D | 0016846 | molecular_function | carbon-sulfur lyase activity |
| D | 0046294 | biological_process | formaldehyde catabolic process |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051907 | molecular_function | S-(hydroxymethyl)glutathione synthase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 200 |
| Chain | Residue |
| A | CYS33 |
| A | CYS35 |
| A | CYS101 |
| A | CYS104 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 200 |
| Chain | Residue |
| B | CYS33 |
| B | CYS35 |
| B | CYS101 |
| B | CYS104 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN C 200 |
| Chain | Residue |
| C | CYS35 |
| C | CYS101 |
| C | CYS104 |
| C | CYS33 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN D 200 |
| Chain | Residue |
| D | CYS33 |
| D | CYS35 |
| D | CYS101 |
| D | CYS104 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 509 |
| Chain | Residue |
| A | PRO177 |
| D | ARG161 |
| D | ARG165 |
| D | HOH644 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 500 |
| Chain | Residue |
| C | ALA14 |
| C | LYS20 |
| C | GLN69 |
| C | GLU128 |
| C | HOH635 |
| C | HOH640 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 D 501 |
| Chain | Residue |
| D | ALA14 |
| D | LYS20 |
| D | GLN69 |
| D | GLU128 |
| D | HOH648 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 502 |
| Chain | Residue |
| A | SER7 |
| A | GLY8 |
| A | LYS10 |
| A | HOH547 |
| B | LYS20 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 503 |
| Chain | Residue |
| A | LYS20 |
| A | ASP133 |
| B | SER7 |
| B | GLY8 |
| B | HOH696 |
| site_id | BC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE GSH A 201 |
| Chain | Residue |
| A | CYS56 |
| A | THR57 |
| A | LYS58 |
| A | PRO95 |
| A | ILE96 |
| A | ARG98 |
| A | PHE143 |
| A | SER146 |
| A | GLU149 |
| A | HOH560 |
| A | HOH605 |
| site_id | BC2 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE GSH B 201 |
| Chain | Residue |
| B | CYS56 |
| B | THR57 |
| B | LYS58 |
| B | ALA94 |
| B | PRO95 |
| B | ILE96 |
| B | ARG98 |
| B | PHE143 |
| B | SER146 |
| B | GLU149 |
| B | HOH703 |
| B | HOH704 |
| site_id | BC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GSH C 201 |
| Chain | Residue |
| C | CYS56 |
| C | THR57 |
| C | LYS58 |
| C | PRO95 |
| C | ILE96 |
| C | ARG98 |
| C | PHE143 |
| C | SER146 |
| C | GLU149 |
| site_id | BC4 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE GSH D 201 |
| Chain | Residue |
| D | CYS56 |
| D | THR57 |
| D | LYS58 |
| D | ALA94 |
| D | PRO95 |
| D | ILE96 |
| D | PHE143 |
| D | SER146 |
| D | HOH659 |
| D | HOH660 |
| D | HOH661 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE GOL B 612 |
| Chain | Residue |
| B | GLN47 |
| B | GLY74 |
| B | HOH630 |
| site_id | BC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE GOL C 613 |
| Chain | Residue |
| C | PRO118 |
| C | LEU179 |
| C | ALA182 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL D 614 |
| Chain | Residue |
| D | HIS50 |
| D | LEU179 |
| D | ALA182 |
| D | HOH622 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 588 |
| Details | Domain: {"description":"CENP-V/GFA","evidences":[{"source":"PROSITE-ProRule","id":"PRU01239","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 28 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01239","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | a catalytic site defined by CSA, PubMed 15548539 |
| Chain | Residue | Details |
| A | CYS56 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | a catalytic site defined by CSA, PubMed 15548539 |
| Chain | Residue | Details |
| B | CYS56 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | a catalytic site defined by CSA, PubMed 15548539 |
| Chain | Residue | Details |
| C | CYS56 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | a catalytic site defined by CSA, PubMed 15548539 |
| Chain | Residue | Details |
| D | CYS56 |
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 712 |
| Chain | Residue | Details |
| A | CYS54 | metal ligand |
| A | CYS56 | metal ligand, nucleofuge, nucleophile |
| A | CYS59 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 3 |
| Details | M-CSA 712 |
| Chain | Residue | Details |
| B | CYS54 | metal ligand |
| B | CYS56 | metal ligand, nucleofuge, nucleophile |
| B | CYS59 | metal ligand |
| site_id | MCSA3 |
| Number of Residues | 3 |
| Details | M-CSA 712 |
| Chain | Residue | Details |
| C | CYS54 | metal ligand |
| C | CYS56 | metal ligand, nucleofuge, nucleophile |
| C | CYS59 | metal ligand |
| site_id | MCSA4 |
| Number of Residues | 3 |
| Details | M-CSA 712 |
| Chain | Residue | Details |
| D | CYS54 | metal ligand |
| D | CYS56 | metal ligand, nucleofuge, nucleophile |
| D | CYS59 | metal ligand |
