1XA8
Crystal Structure Analysis of Glutathione-dependent formaldehyde-activating enzyme (Gfa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.910, 120.620, 97.140 |
| Unit cell angles | 90.00, 97.68, 90.00 |
Refinement procedure
| Resolution | 75.160 - 2.400 |
| R-factor | 0.19889 |
| Rwork | 0.195 |
| R-free | 0.24784 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x6m |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.095 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.270 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.319 |
| Number of reflections | 48095 | |
| <I/σ(I)> | 7.87 | 2.16 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 1.96 | 1.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | (NH4)2SO4, HEPES, PEG400, 2-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
