1WSI

Crystal structure of E.coli RNase HI active site mutant (E48A/K87A/D134N)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006401	biological_process	RNA catabolic process
A	0016787	molecular_function	hydrolase activity
A	0043137	biological_process	DNA replication, removal of RNA primer
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0003676	molecular_function	nucleic acid binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006401	biological_process	RNA catabolic process
B	0016787	molecular_function	hydrolase activity
B	0043137	biological_process	DNA replication, removal of RNA primer
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0003676	molecular_function	nucleic acid binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0006401	biological_process	RNA catabolic process
C	0016787	molecular_function	hydrolase activity
C	0043137	biological_process	DNA replication, removal of RNA primer
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0003676	molecular_function	nucleic acid binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0006401	biological_process	RNA catabolic process
D	0016787	molecular_function	hydrolase activity
D	0043137	biological_process	DNA replication, removal of RNA primer
D	0046872	molecular_function	metal ion binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"1311386","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 163

Chain	Residue	Details
A	ASP10	metal ligand
A	GLY11	metal ligand
A	ALA48	increase acidity, increase basicity, unknown
A	ASP70	metal ligand
A	ASN134	increase acidity, metal ligand, proton acceptor, proton donor

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site_id	MCSA2
Number of Residues	5
Details	M-CSA 163

Chain	Residue	Details
B	ASP10	metal ligand
B	GLY11	metal ligand
B	ALA48	increase acidity, increase basicity, unknown
B	ASP70	metal ligand
B	ASN134	increase acidity, metal ligand, proton acceptor, proton donor

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site_id	MCSA3
Number of Residues	5
Details	M-CSA 163

Chain	Residue	Details
C	ASP10	metal ligand
C	GLY11	metal ligand
C	ALA48	increase acidity, increase basicity, unknown
C	ASP70	metal ligand
C	ASN134	increase acidity, metal ligand, proton acceptor, proton donor

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site_id	MCSA4
Number of Residues	5
Details	M-CSA 163

Chain	Residue	Details
D	ASP10	metal ligand
D	GLY11	metal ligand
D	ALA48	increase acidity, increase basicity, unknown
D	ASP70	metal ligand
D	ASN134	increase acidity, metal ligand, proton acceptor, proton donor

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