1WCQ
Mutagenesis of the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004308 | molecular_function | exo-alpha-sialidase activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0006689 | biological_process | ganglioside catabolic process |
| A | 0009313 | biological_process | oligosaccharide catabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| A | 0052794 | molecular_function | obsolete exo-alpha-(2->3)-sialidase activity |
| A | 0052795 | molecular_function | obsolete exo-alpha-(2->6)-sialidase activity |
| A | 0052796 | molecular_function | obsolete exo-alpha-(2->8)-sialidase activity |
| B | 0004308 | molecular_function | exo-alpha-sialidase activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0006689 | biological_process | ganglioside catabolic process |
| B | 0009313 | biological_process | oligosaccharide catabolic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| B | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| B | 0052794 | molecular_function | obsolete exo-alpha-(2->3)-sialidase activity |
| B | 0052795 | molecular_function | obsolete exo-alpha-(2->6)-sialidase activity |
| B | 0052796 | molecular_function | obsolete exo-alpha-(2->8)-sialidase activity |
| C | 0004308 | molecular_function | exo-alpha-sialidase activity |
| C | 0005576 | cellular_component | extracellular region |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0006689 | biological_process | ganglioside catabolic process |
| C | 0009313 | biological_process | oligosaccharide catabolic process |
| C | 0016020 | cellular_component | membrane |
| C | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| C | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| C | 0052794 | molecular_function | obsolete exo-alpha-(2->3)-sialidase activity |
| C | 0052795 | molecular_function | obsolete exo-alpha-(2->6)-sialidase activity |
| C | 0052796 | molecular_function | obsolete exo-alpha-(2->8)-sialidase activity |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 3 |
| Details | ACT_SITE: Proton acceptor => ECO:0000250 |
| Chain | Residue | Details |
| A | ASP92 | |
| B | ASP92 | |
| C | ASP92 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | ACT_SITE: Nucleophile => ECO:0000255 |
| Chain | Residue | Details |
| A | GLU260 | |
| B | GLU260 | |
| C | GLU260 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | ACT_SITE: Nucleophile |
| Chain | Residue | Details |
| A | PHE370 | |
| B | PHE370 | |
| C | PHE370 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | ARG68 | |
| A | ARG276 | |
| B | ARG68 | |
| B | ARG276 | |
| C | ARG68 | |
| C | ARG276 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1euu |
| Chain | Residue | Details |
| A | ASP92 | |
| A | PHE370 | |
| A | GLU260 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1euu |
| Chain | Residue | Details |
| B | ASP92 | |
| B | PHE370 | |
| B | GLU260 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1euu |
| Chain | Residue | Details |
| C | ASP92 | |
| C | PHE370 | |
| C | GLU260 |
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 835 |
| Chain | Residue | Details |
| A | ASP92 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| A | GLU260 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| A | PHE370 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| site_id | MCSA2 |
| Number of Residues | 3 |
| Details | M-CSA 835 |
| Chain | Residue | Details |
| B | ASP92 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| B | GLU260 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| B | PHE370 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| site_id | MCSA3 |
| Number of Residues | 3 |
| Details | M-CSA 835 |
| Chain | Residue | Details |
| C | ASP92 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| C | GLU260 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| C | PHE370 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
