1W55

Structure of the Bifunctional IspDF from Campylobacter jejuni

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0008299	biological_process	isoprenoid biosynthetic process
A	0008685	molecular_function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
A	0016114	biological_process	terpenoid biosynthetic process
A	0016779	molecular_function	nucleotidyltransferase activity
A	0016829	molecular_function	lyase activity
A	0019288	biological_process	isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
A	0046872	molecular_function	metal ion binding
A	0050518	molecular_function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
A	0070567	molecular_function	cytidylyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A1371

Chain	Residue
A	ASP217
A	THR341
A	C1372
A	HOH2226
A	HOH2244
A	HOH2245

---

site_id	AC2
Number of Residues	19
Details	BINDING SITE FOR RESIDUE C A1372

Chain	Residue
A	ALA309
A	PRO312
A	LYS313
A	LEU314
A	LYS315
A	LYS318
A	ALA340
A	THR341
A	THR342
A	MG1371
A	HOH2168
A	HOH2240
A	HOH2241
A	HOH2242
A	HOH2244
A	HOH2245
A	HIS243
A	ASP265
A	GLY267

---

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE C A1373

Chain	Residue
A	LEU9
A	ALA10
A	ALA11
A	THR53
A	GLY73
A	ASP74
A	THR75
A	ARG76
A	SER79
A	ASP96
A	VAL97
A	ARG139
A	LYS191
A	HOH2048
A	HOH2112
A	HOH2246
A	HOH2247
A	HOH2248

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GPP A1374

Chain	Residue
A	PHE216
A	MET308
A	GLY347
A	PHE348
A	ARG351
A	HOH2228

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A1375

Chain	Residue
A	ASP96
A	ARG99
A	ASP128
A	THR129
A	ILE145
A	THR167
A	LYS191
A	HOH2112

---

Functional Information from PROSITE/UniProt

site_id	PS01295
Number of Residues	8
Details	ISPD 4-diphosphocytidyl-2C-methyl-D-erythritol synthase signature. VMVSDVAR

Chain	Residue	Details
A	VAL92-ARG99

---

site_id	PS01350
Number of Residues	16
Details	ISPF 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase signature. SDGDVLaHSltDAilG

Chain	Residue	Details
A	SER244-GLY259

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	9
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P62617, ECO:0000255|HAMAP-Rule:MF_01520

Chain	Residue	Details
A	ASP217
A	HIS219
A	HIS243
A	HIS251
A	ASP265
A	TYR270
A	THR341
A	PHE348
A	ARG351

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P62617

Chain	Residue	Details
A	ALA309

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Transition state stabilizer => ECO:0000255|HAMAP-Rule:MF_01520

Chain	Residue	Details
A	ARG16
A	LYS23

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Positions MEP for the nucleophilic attack => ECO:0000255|HAMAP-Rule:MF_01520

Chain	Residue	Details
A	ARG139
A	LYS191

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Transition state stabilizer => ECO:0000250|UniProtKB:P62617, ECO:0000255|HAMAP-Rule:MF_01520

Chain	Residue	Details
A	HIS243
A	THR342

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1hv9

Chain	Residue	Details
A	ARG16

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