1W55
Structure of the Bifunctional IspDF from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2002-06-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 108.758, 108.758, 161.262 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.960 - 2.300 |
| R-factor | 0.17 |
| Rwork | 0.167 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1I52 AND 1GX1 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 1.744 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.960 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 489834 | |
| <I/σ(I)> | 41.5 | 5.3 |
| Completeness [%] | 98.4 | 87.2 |
| Redundancy | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 35 % ETHYLENE GLYCOL |
