1VYJ

Structural and biochemical studies of human PCNA complexes provide the basis for association with CDK/cyclin and rationale for inhibitor design

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
A	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
A	0000781	cellular_component	chromosome, telomeric region
A	0000785	cellular_component	chromatin
A	0001673	cellular_component	male germ cell nucleus
A	0003677	molecular_function	DNA binding
A	0003682	molecular_function	chromatin binding
A	0003684	molecular_function	damaged DNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005652	cellular_component	nuclear lamina
A	0005654	cellular_component	nucleoplasm
A	0005657	cellular_component	replication fork
A	0005813	cellular_component	centrosome
A	0006260	biological_process	DNA replication
A	0006272	biological_process	leading strand elongation
A	0006275	biological_process	regulation of DNA replication
A	0006281	biological_process	DNA repair
A	0006287	biological_process	base-excision repair, gap-filling
A	0006298	biological_process	mismatch repair
A	0006974	biological_process	DNA damage response
A	0006979	biological_process	response to oxidative stress
A	0007507	biological_process	heart development
A	0016604	cellular_component	nuclear body
A	0019899	molecular_function	enzyme binding
A	0019985	biological_process	translesion synthesis
A	0030331	molecular_function	nuclear estrogen receptor binding
A	0030337	molecular_function	DNA polymerase processivity factor activity
A	0030855	biological_process	epithelial cell differentiation
A	0030894	cellular_component	replisome
A	0030971	molecular_function	receptor tyrosine kinase binding
A	0031297	biological_process	replication fork processing
A	0032077	biological_process	positive regulation of deoxyribonuclease activity
A	0032139	molecular_function	dinucleotide insertion or deletion binding
A	0032355	biological_process	response to estradiol
A	0032405	molecular_function	MutLalpha complex binding
A	0033993	biological_process	response to lipid
A	0034644	biological_process	cellular response to UV
A	0035035	molecular_function	histone acetyltransferase binding
A	0042802	molecular_function	identical protein binding
A	0043596	cellular_component	nuclear replication fork
A	0043626	cellular_component	PCNA complex
A	0044849	biological_process	estrous cycle
A	0044877	molecular_function	protein-containing complex binding
A	0045739	biological_process	positive regulation of DNA repair
A	0045740	biological_process	positive regulation of DNA replication
A	0046686	biological_process	response to cadmium ion
A	0070062	cellular_component	extracellular exosome
A	0070182	molecular_function	DNA polymerase binding
A	0070301	biological_process	cellular response to hydrogen peroxide
A	0070557	cellular_component	PCNA-p21 complex
A	0071466	biological_process	cellular response to xenobiotic stimulus
A	0071548	biological_process	response to dexamethasone
A	0097421	biological_process	liver regeneration
A	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
A	1902065	biological_process	response to L-glutamate
A	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
C	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
C	0000781	cellular_component	chromosome, telomeric region
C	0000785	cellular_component	chromatin
C	0001673	cellular_component	male germ cell nucleus
C	0003677	molecular_function	DNA binding
C	0003682	molecular_function	chromatin binding
C	0003684	molecular_function	damaged DNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005652	cellular_component	nuclear lamina
C	0005654	cellular_component	nucleoplasm
C	0005657	cellular_component	replication fork
C	0005813	cellular_component	centrosome
C	0006260	biological_process	DNA replication
C	0006272	biological_process	leading strand elongation
C	0006275	biological_process	regulation of DNA replication
C	0006281	biological_process	DNA repair
C	0006287	biological_process	base-excision repair, gap-filling
C	0006298	biological_process	mismatch repair
C	0006974	biological_process	DNA damage response
C	0006979	biological_process	response to oxidative stress
C	0007507	biological_process	heart development
C	0016604	cellular_component	nuclear body
C	0019899	molecular_function	enzyme binding
C	0019985	biological_process	translesion synthesis
C	0030331	molecular_function	nuclear estrogen receptor binding
C	0030337	molecular_function	DNA polymerase processivity factor activity
C	0030855	biological_process	epithelial cell differentiation
C	0030894	cellular_component	replisome
C	0030971	molecular_function	receptor tyrosine kinase binding
C	0031297	biological_process	replication fork processing
C	0032077	biological_process	positive regulation of deoxyribonuclease activity
C	0032139	molecular_function	dinucleotide insertion or deletion binding
C	0032355	biological_process	response to estradiol
C	0032405	molecular_function	MutLalpha complex binding
C	0033993	biological_process	response to lipid
C	0034644	biological_process	cellular response to UV
C	0035035	molecular_function	histone acetyltransferase binding
C	0042802	molecular_function	identical protein binding
C	0043596	cellular_component	nuclear replication fork
C	0043626	cellular_component	PCNA complex
C	0044849	biological_process	estrous cycle
C	0044877	molecular_function	protein-containing complex binding
C	0045739	biological_process	positive regulation of DNA repair
C	0045740	biological_process	positive regulation of DNA replication
C	0046686	biological_process	response to cadmium ion
C	0070062	cellular_component	extracellular exosome
C	0070182	molecular_function	DNA polymerase binding
C	0070301	biological_process	cellular response to hydrogen peroxide
C	0070557	cellular_component	PCNA-p21 complex
C	0071466	biological_process	cellular response to xenobiotic stimulus
C	0071548	biological_process	response to dexamethasone
C	0097421	biological_process	liver regeneration
C	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
C	1902065	biological_process	response to L-glutamate
C	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
E	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
E	0000781	cellular_component	chromosome, telomeric region
E	0000785	cellular_component	chromatin
E	0001673	cellular_component	male germ cell nucleus
E	0003677	molecular_function	DNA binding
E	0003682	molecular_function	chromatin binding
E	0003684	molecular_function	damaged DNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005652	cellular_component	nuclear lamina
E	0005654	cellular_component	nucleoplasm
E	0005657	cellular_component	replication fork
E	0005813	cellular_component	centrosome
E	0006260	biological_process	DNA replication
E	0006272	biological_process	leading strand elongation
E	0006275	biological_process	regulation of DNA replication
E	0006281	biological_process	DNA repair
E	0006287	biological_process	base-excision repair, gap-filling
E	0006298	biological_process	mismatch repair
E	0006974	biological_process	DNA damage response
E	0006979	biological_process	response to oxidative stress
E	0007507	biological_process	heart development
E	0016604	cellular_component	nuclear body
E	0019899	molecular_function	enzyme binding
E	0019985	biological_process	translesion synthesis
E	0030331	molecular_function	nuclear estrogen receptor binding
E	0030337	molecular_function	DNA polymerase processivity factor activity
E	0030855	biological_process	epithelial cell differentiation
E	0030894	cellular_component	replisome
E	0030971	molecular_function	receptor tyrosine kinase binding
E	0031297	biological_process	replication fork processing
E	0032077	biological_process	positive regulation of deoxyribonuclease activity
E	0032139	molecular_function	dinucleotide insertion or deletion binding
E	0032355	biological_process	response to estradiol
E	0032405	molecular_function	MutLalpha complex binding
E	0033993	biological_process	response to lipid
E	0034644	biological_process	cellular response to UV
E	0035035	molecular_function	histone acetyltransferase binding
E	0042802	molecular_function	identical protein binding
E	0043596	cellular_component	nuclear replication fork
E	0043626	cellular_component	PCNA complex
E	0044849	biological_process	estrous cycle
E	0044877	molecular_function	protein-containing complex binding
E	0045739	biological_process	positive regulation of DNA repair
E	0045740	biological_process	positive regulation of DNA replication
E	0046686	biological_process	response to cadmium ion
E	0070062	cellular_component	extracellular exosome
E	0070182	molecular_function	DNA polymerase binding
E	0070301	biological_process	cellular response to hydrogen peroxide
E	0070557	cellular_component	PCNA-p21 complex
E	0071466	biological_process	cellular response to xenobiotic stimulus
E	0071548	biological_process	response to dexamethasone
E	0097421	biological_process	liver regeneration
E	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
E	1902065	biological_process	response to L-glutamate
E	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
G	0000122	biological_process	negative regulation of transcription by RNA polymerase II
G	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
G	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
G	0000781	cellular_component	chromosome, telomeric region
G	0000785	cellular_component	chromatin
G	0001673	cellular_component	male germ cell nucleus
G	0003677	molecular_function	DNA binding
G	0003682	molecular_function	chromatin binding
G	0003684	molecular_function	damaged DNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005652	cellular_component	nuclear lamina
G	0005654	cellular_component	nucleoplasm
G	0005657	cellular_component	replication fork
G	0005813	cellular_component	centrosome
G	0006260	biological_process	DNA replication
G	0006272	biological_process	leading strand elongation
G	0006275	biological_process	regulation of DNA replication
G	0006281	biological_process	DNA repair
G	0006287	biological_process	base-excision repair, gap-filling
G	0006298	biological_process	mismatch repair
G	0006974	biological_process	DNA damage response
G	0006979	biological_process	response to oxidative stress
G	0007507	biological_process	heart development
G	0016604	cellular_component	nuclear body
G	0019899	molecular_function	enzyme binding
G	0019985	biological_process	translesion synthesis
G	0030331	molecular_function	nuclear estrogen receptor binding
G	0030337	molecular_function	DNA polymerase processivity factor activity
G	0030855	biological_process	epithelial cell differentiation
G	0030894	cellular_component	replisome
G	0030971	molecular_function	receptor tyrosine kinase binding
G	0031297	biological_process	replication fork processing
G	0032077	biological_process	positive regulation of deoxyribonuclease activity
G	0032139	molecular_function	dinucleotide insertion or deletion binding
G	0032355	biological_process	response to estradiol
G	0032405	molecular_function	MutLalpha complex binding
G	0033993	biological_process	response to lipid
G	0034644	biological_process	cellular response to UV
G	0035035	molecular_function	histone acetyltransferase binding
G	0042802	molecular_function	identical protein binding
G	0043596	cellular_component	nuclear replication fork
G	0043626	cellular_component	PCNA complex
G	0044849	biological_process	estrous cycle
G	0044877	molecular_function	protein-containing complex binding
G	0045739	biological_process	positive regulation of DNA repair
G	0045740	biological_process	positive regulation of DNA replication
G	0046686	biological_process	response to cadmium ion
G	0070062	cellular_component	extracellular exosome
G	0070182	molecular_function	DNA polymerase binding
G	0070301	biological_process	cellular response to hydrogen peroxide
G	0070557	cellular_component	PCNA-p21 complex
G	0071466	biological_process	cellular response to xenobiotic stimulus
G	0071548	biological_process	response to dexamethasone
G	0097421	biological_process	liver regeneration
G	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
G	1902065	biological_process	response to L-glutamate
G	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
I	0000122	biological_process	negative regulation of transcription by RNA polymerase II
I	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
I	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
I	0000781	cellular_component	chromosome, telomeric region
I	0000785	cellular_component	chromatin
I	0001673	cellular_component	male germ cell nucleus
I	0003677	molecular_function	DNA binding
I	0003682	molecular_function	chromatin binding
I	0003684	molecular_function	damaged DNA binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005652	cellular_component	nuclear lamina
I	0005654	cellular_component	nucleoplasm
I	0005657	cellular_component	replication fork
I	0005813	cellular_component	centrosome
I	0006260	biological_process	DNA replication
I	0006272	biological_process	leading strand elongation
I	0006275	biological_process	regulation of DNA replication
I	0006281	biological_process	DNA repair
I	0006287	biological_process	base-excision repair, gap-filling
I	0006298	biological_process	mismatch repair
I	0006974	biological_process	DNA damage response
I	0006979	biological_process	response to oxidative stress
I	0007507	biological_process	heart development
I	0016604	cellular_component	nuclear body
I	0019899	molecular_function	enzyme binding
I	0019985	biological_process	translesion synthesis
I	0030331	molecular_function	nuclear estrogen receptor binding
I	0030337	molecular_function	DNA polymerase processivity factor activity
I	0030855	biological_process	epithelial cell differentiation
I	0030894	cellular_component	replisome
I	0030971	molecular_function	receptor tyrosine kinase binding
I	0031297	biological_process	replication fork processing
I	0032077	biological_process	positive regulation of deoxyribonuclease activity
I	0032139	molecular_function	dinucleotide insertion or deletion binding
I	0032355	biological_process	response to estradiol
I	0032405	molecular_function	MutLalpha complex binding
I	0033993	biological_process	response to lipid
I	0034644	biological_process	cellular response to UV
I	0035035	molecular_function	histone acetyltransferase binding
I	0042802	molecular_function	identical protein binding
I	0043596	cellular_component	nuclear replication fork
I	0043626	cellular_component	PCNA complex
I	0044849	biological_process	estrous cycle
I	0044877	molecular_function	protein-containing complex binding
I	0045739	biological_process	positive regulation of DNA repair
I	0045740	biological_process	positive regulation of DNA replication
I	0046686	biological_process	response to cadmium ion
I	0070062	cellular_component	extracellular exosome
I	0070182	molecular_function	DNA polymerase binding
I	0070301	biological_process	cellular response to hydrogen peroxide
I	0070557	cellular_component	PCNA-p21 complex
I	0071466	biological_process	cellular response to xenobiotic stimulus
I	0071548	biological_process	response to dexamethasone
I	0097421	biological_process	liver regeneration
I	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
I	1902065	biological_process	response to L-glutamate
I	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
K	0000122	biological_process	negative regulation of transcription by RNA polymerase II
K	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
K	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
K	0000781	cellular_component	chromosome, telomeric region
K	0000785	cellular_component	chromatin
K	0001673	cellular_component	male germ cell nucleus
K	0003677	molecular_function	DNA binding
K	0003682	molecular_function	chromatin binding
K	0003684	molecular_function	damaged DNA binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005652	cellular_component	nuclear lamina
K	0005654	cellular_component	nucleoplasm
K	0005657	cellular_component	replication fork
K	0005813	cellular_component	centrosome
K	0006260	biological_process	DNA replication
K	0006272	biological_process	leading strand elongation
K	0006275	biological_process	regulation of DNA replication
K	0006281	biological_process	DNA repair
K	0006287	biological_process	base-excision repair, gap-filling
K	0006298	biological_process	mismatch repair
K	0006974	biological_process	DNA damage response
K	0006979	biological_process	response to oxidative stress
K	0007507	biological_process	heart development
K	0016604	cellular_component	nuclear body
K	0019899	molecular_function	enzyme binding
K	0019985	biological_process	translesion synthesis
K	0030331	molecular_function	nuclear estrogen receptor binding
K	0030337	molecular_function	DNA polymerase processivity factor activity
K	0030855	biological_process	epithelial cell differentiation
K	0030894	cellular_component	replisome
K	0030971	molecular_function	receptor tyrosine kinase binding
K	0031297	biological_process	replication fork processing
K	0032077	biological_process	positive regulation of deoxyribonuclease activity
K	0032139	molecular_function	dinucleotide insertion or deletion binding
K	0032355	biological_process	response to estradiol
K	0032405	molecular_function	MutLalpha complex binding
K	0033993	biological_process	response to lipid
K	0034644	biological_process	cellular response to UV
K	0035035	molecular_function	histone acetyltransferase binding
K	0042802	molecular_function	identical protein binding
K	0043596	cellular_component	nuclear replication fork
K	0043626	cellular_component	PCNA complex
K	0044849	biological_process	estrous cycle
K	0044877	molecular_function	protein-containing complex binding
K	0045739	biological_process	positive regulation of DNA repair
K	0045740	biological_process	positive regulation of DNA replication
K	0046686	biological_process	response to cadmium ion
K	0070062	cellular_component	extracellular exosome
K	0070182	molecular_function	DNA polymerase binding
K	0070301	biological_process	cellular response to hydrogen peroxide
K	0070557	cellular_component	PCNA-p21 complex
K	0071466	biological_process	cellular response to xenobiotic stimulus
K	0071548	biological_process	response to dexamethasone
K	0097421	biological_process	liver regeneration
K	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
K	1902065	biological_process	response to L-glutamate
K	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication

Functional Information from PROSITE/UniProt

site_id	PS00293
Number of Residues	19
Details	PCNA_2 Proliferating cell nuclear antigen signature 2. RCDRnlaMgvnLtSMsKIL

Chain	Residue	Details
A	ARG61-LEU79

---

site_id	PS01251
Number of Residues	24
Details	PCNA_1 Proliferating cell nuclear antigen signature 1. GVnLqSMDsSHVsLVqLtLrsegF

Chain	Residue	Details
A	GLY34-PHE57

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	114
Details	DNA_BIND: DNA_BIND => ECO:0000255

Chain	Residue	Details
A	ARG61-LYS80
C	ARG61-LYS80
E	ARG61-LYS80
G	ARG61-LYS80
I	ARG61-LYS80
K	ARG61-LYS80

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:19419956

Chain	Residue	Details
A	LYS14
C	LYS14
E	LYS14
G	LYS14
I	LYS14
K	LYS14

---

site_id	SWS_FT_FI3
Number of Residues	18
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS77
G	LYS77
G	LYS80
G	LYS248
I	LYS77
I	LYS80
I	LYS248
K	LYS77
K	LYS80
K	LYS248
A	LYS80
A	LYS248
C	LYS77
C	LYS80
C	LYS248
E	LYS77
E	LYS80
E	LYS248

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: Phosphotyrosine; by EGFR => ECO:0000269|PubMed:17115032

Chain	Residue	Details
A	TYR211
C	TYR211
E	TYR211
G	TYR211
I	TYR211
K	TYR211

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269|PubMed:17108083, ECO:0000269|PubMed:17130289

Chain	Residue	Details
A	LYS164
C	LYS164
E	LYS164
G	LYS164
I	LYS164
K	LYS164

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
I	LYS254
K	LYS254
A	LYS254
C	LYS254
E	LYS254
G	LYS254

---