1V0E
Endosialidase of Bacteriophage K1F
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0016996 | molecular_function | endo-alpha-(2,8)-sialidase activity |
| B | 0016996 | molecular_function | endo-alpha-(2,8)-sialidase activity |
| C | 0016996 | molecular_function | endo-alpha-(2,8)-sialidase activity |
| D | 0016996 | molecular_function | endo-alpha-(2,8)-sialidase activity |
| E | 0016996 | molecular_function | endo-alpha-(2,8)-sialidase activity |
| F | 0016996 | molecular_function | endo-alpha-(2,8)-sialidase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PO4 B1685 |
| Chain | Residue |
| A | PRO829 |
| A | THR830 |
| B | GLU818 |
| B | GLY819 |
| B | HOH2478 |
| B | HOH2534 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 C1685 |
| Chain | Residue |
| C | GLU818 |
| C | GLY819 |
| C | HOH2456 |
| C | HOH2515 |
| B | PRO829 |
| B | THR830 |
| B | HOH2482 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 C1686 |
| Chain | Residue |
| A | GLY819 |
| A | HOH2470 |
| C | PRO829 |
| C | THR830 |
| C | HOH2516 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 D1685 |
| Chain | Residue |
| D | GLU818 |
| D | GLY819 |
| D | HOH2468 |
| D | HOH2529 |
| E | PRO829 |
| E | THR830 |
| E | HOH2406 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 F1685 |
| Chain | Residue |
| D | ALA805 |
| E | GLU818 |
| E | GLY819 |
| E | HOH2401 |
| F | PRO829 |
| F | THR830 |
| F | HOH2508 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 F1686 |
| Chain | Residue |
| D | PRO829 |
| D | THR830 |
| D | HOH2471 |
| E | ALA805 |
| F | GLU818 |
| F | GLY819 |
| F | HOH2509 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:15608653, ECO:0000269|PubMed:20096705 |
| Chain | Residue | Details |
| A | GLU581 | |
| E | ARG647 | |
| F | GLU581 | |
| F | ARG647 | |
| A | ARG647 | |
| B | GLU581 | |
| B | ARG647 | |
| C | GLU581 | |
| C | ARG647 | |
| D | GLU581 | |
| D | ARG647 | |
| E | GLU581 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:15608653 |
| Chain | Residue | Details |
| A | ARG596 | |
| B | ARG596 | |
| C | ARG596 | |
| D | ARG596 | |
| E | ARG596 | |
| F | ARG596 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 36 |
| Details | SITE: Binding to sialic acid => ECO:0000269|PubMed:20096705 |
| Chain | Residue | Details |
| A | HIS542 | |
| B | ASP578 | |
| B | THR598 | |
| B | ARG599 | |
| C | HIS542 | |
| C | ASP545 | |
| C | ARG549 | |
| C | ASP578 | |
| C | THR598 | |
| C | ARG599 | |
| D | HIS542 | |
| A | ASP545 | |
| D | ASP545 | |
| D | ARG549 | |
| D | ASP578 | |
| D | THR598 | |
| D | ARG599 | |
| E | HIS542 | |
| E | ASP545 | |
| E | ARG549 | |
| E | ASP578 | |
| E | THR598 | |
| A | ARG549 | |
| E | ARG599 | |
| F | HIS542 | |
| F | ASP545 | |
| F | ARG549 | |
| F | ASP578 | |
| F | THR598 | |
| F | ARG599 | |
| A | ASP578 | |
| A | THR598 | |
| A | ARG599 | |
| B | HIS542 | |
| B | ASP545 | |
| B | ARG549 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 15608653, 8089131 |
| Chain | Residue | Details |
| A | GLU581 | |
| A | ARG596 | |
| A | ARG647 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 15608653, 8089131 |
| Chain | Residue | Details |
| B | GLU581 | |
| B | ARG596 | |
| B | ARG647 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 15608653, 8089131 |
| Chain | Residue | Details |
| C | GLU581 | |
| C | ARG596 | |
| C | ARG647 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 15608653, 8089131 |
| Chain | Residue | Details |
| D | GLU581 | |
| D | ARG596 | |
| D | ARG647 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 15608653, 8089131 |
| Chain | Residue | Details |
| E | GLU581 | |
| E | ARG596 | |
| E | ARG647 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | a catalytic site defined by CSA, PubMed 15608653, 8089131 |
| Chain | Residue | Details |
| F | GLU581 | |
| F | ARG596 | |
| F | ARG647 |
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| A | TYR325 | promote heterolysis, proton acceptor, proton donor |
| A | HIS350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| A | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| A | ARG596 | electrostatic stabiliser, increase acidity |
| A | ARG647 | electrostatic stabiliser, increase acidity |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| B | TYR325 | promote heterolysis, proton acceptor, proton donor |
| B | HIS350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| B | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| B | ARG596 | electrostatic stabiliser, increase acidity |
| B | ARG647 | electrostatic stabiliser, increase acidity |
| site_id | MCSA3 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| C | TYR325 | promote heterolysis, proton acceptor, proton donor |
| C | HIS350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| C | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| C | ARG596 | electrostatic stabiliser, increase acidity |
| C | ARG647 | electrostatic stabiliser, increase acidity |
| site_id | MCSA4 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| D | TYR325 | promote heterolysis, proton acceptor, proton donor |
| D | HIS350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| D | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| D | ARG596 | electrostatic stabiliser, increase acidity |
| D | ARG647 | electrostatic stabiliser, increase acidity |
| site_id | MCSA5 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| E | TYR325 | promote heterolysis, proton acceptor, proton donor |
| E | HIS350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| E | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| E | ARG596 | electrostatic stabiliser, increase acidity |
| E | ARG647 | electrostatic stabiliser, increase acidity |
| site_id | MCSA6 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| F | TYR325 | promote heterolysis, proton acceptor, proton donor |
| F | HIS350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| F | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| F | ARG596 | electrostatic stabiliser, increase acidity |
| F | ARG647 | electrostatic stabiliser, increase acidity |
