Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1UXJ

Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005737	cellular_component	cytoplasm
A	0006099	biological_process	tricarboxylic acid cycle
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019752	biological_process	carboxylic acid metabolic process
A	0030060	molecular_function	L-malate dehydrogenase (NAD+) activity
C	0003824	molecular_function	catalytic activity
C	0005737	cellular_component	cytoplasm
C	0006099	biological_process	tricarboxylic acid cycle
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019752	biological_process	carboxylic acid metabolic process
C	0030060	molecular_function	L-malate dehydrogenase (NAD+) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A 1309

Chain	Residue
A	ASP200
A	ASP243
A	GLU277
A	HOH2284

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD A 1310

Chain	Residue
A	GLU195
A	GLU281
A	HOH2211
C	GLU281
C	HOH2278

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A 1311

Chain	Residue
A	GLU178
A	CL1313
A	HOH2154
A	HOH2195

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD A 1312

Chain	Residue
A	ASP148
A	HIS175
A	HOH2168
A	HOH2172
A	HOH2173

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL A 1313

Chain	Residue
A	ASP123
A	ALA124
A	THR302
A	CD1311

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NA A 1314

Chain	Residue
A	HIS19
A	HIS19
A	HOH2017
A	HOH2017

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE NA A 1315

Chain	Residue
A	HOH2251
A	HOH2251

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD C 1311

Chain	Residue
C	GLU178
C	CL1314
C	HOH2137
C	HOH2181

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CD C 1312

Chain	Residue
A	GLU159
A	ARG201
A	HOH2181
C	GLU159
C	ARG201
C	HOH2161

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD C 1313

Chain	Residue
C	ASP148
C	HIS175
C	HOH2153
C	HOH2176
C	HOH2312

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL C 1314

Chain	Residue
C	ASP123
C	ALA124
C	THR302
C	CD1311

site_id	BC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE NA C 1315

Chain	Residue
A	HOH2027
C	GLU238

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD C 1316

Chain	Residue
A	GLU238
A	HOH2256
A	HOH2257
C	HOH2021
C	HOH2168

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD C 1317

Chain	Residue
C	HOH2085
C	HOH2195
C	HOH2251
C	HOH2273
C	HOH2275

site_id	BC6
Number of Residues	32
Details	BINDING SITE FOR RESIDUE NAD A 1308

Chain	Residue
A	GLY9
A	GLY11
A	PHE12
A	VAL13
A	ASP33
A	ILE34
A	VAL35
A	TYR65
A	THR77
A	SER78
A	GLY79
A	PRO81
A	CYS102
A	VAL118
A	ASN119
A	ASN120
A	GLN143
A	LEU147
A	HIS175
A	PRO229
A	HOH2118
A	HOH2168
A	HOH2248
A	HOH2317
A	HOH2318
A	HOH2319
A	HOH2320
A	HOH2321
A	HOH2322
A	HOH2323
A	HOH2324
A	HOH2325

site_id	BC7
Number of Residues	30
Details	BINDING SITE FOR RESIDUE NAD C 1310

Chain	Residue
C	THR77
C	SER78
C	GLY79
C	ILE98
C	CYS102
C	VAL118
C	ASN119
C	ASN120
C	GLN143
C	LEU147
C	HIS175
C	PRO229
C	HOH2096
C	HOH2229
C	HOH2230
C	HOH2308
C	HOH2310
C	HOH2311
C	HOH2312
C	HOH2313
C	HOH2314
C	HOH2315
C	GLY9
C	GLY11
C	PHE12
C	VAL13
C	ASP33
C	ILE34
C	VAL35
C	TYR65

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"UniProtKB","id":"P61889","evidenceCode":"ECO:0000250"},{"source":"HAMAP-Rule","id":"MF_00487","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00487","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12054817","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14636605","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P61889","evidenceCode":"ECO:0000250"},{"source":"HAMAP-Rule","id":"MF_00487","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
A	HIS175
A	ASP148

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
C	HIS175
C	ASP148

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
A	HIS175
A	ASP148
A	ARG151

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
C	HIS175
C	ASP148
C	ARG151

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)