1UWV

Crystal Structure of RumA, the iron-sulfur cluster containing E. coli 23S Ribosomal RNA 5-Methyluridine Methyltransferase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0005506	molecular_function	iron ion binding
A	0006364	biological_process	rRNA processing
A	0006396	biological_process	RNA processing
A	0008168	molecular_function	methyltransferase activity
A	0008173	molecular_function	RNA methyltransferase activity
A	0016740	molecular_function	transferase activity
A	0031167	biological_process	rRNA methylation
A	0032259	biological_process	methylation
A	0046872	molecular_function	metal ion binding
A	0051536	molecular_function	iron-sulfur cluster binding
A	0051539	molecular_function	4 iron, 4 sulfur cluster binding
A	0070041	molecular_function	rRNA (uridine-C5-)-methyltransferase activity
A	0070475	biological_process	rRNA base methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A1436

Chain	Residue
A	NI1434

---

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SF4 A1432

Chain	Residue
A	CYS81
A	CYS87
A	CYS90
A	GLN93
A	GLN161
A	CYS162
A	PRO163
A	ILE164

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NI A1433

Chain	Residue
A	HIS114
A	ILE408
A	HOH2050
A	HOH2116
A	HOH2117

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NI A1434

Chain	Residue
A	ARG132
A	HIS191
A	GLU193
A	CL1436

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 A1435

Chain	Residue
A	ARG132
A	HIS191
A	ARG206
A	SER236
A	LYS273

---

Functional Information from PROSITE/UniProt

site_id	PS01230
Number of Residues	31
Details	TRMA_1 RNA methyltransferase trmA family signature 1. DPARaGaagvmqqiiklepir....IVYvSCNpaT

Chain	Residue	Details
A	ASP363-THR393

---

site_id	PS01231
Number of Residues	11
Details	TRMA_2 RNA methyltransferase trmA family signature 2. LDmFPHTgHLE

Chain	Residue	Details
A	LEU414-GLU424

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	22
Details	Region: {"description":"Interaction with RNA"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"HAMAP-Rule","id":"MF_01010","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15766524","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15016356","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15766524","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15766524","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Site: {"description":"Interaction with RNA"}

Chain

Residue

Details

---