1UWV

Crystal Structure of RumA, the iron-sulfur cluster containing E. coli 23S Ribosomal RNA 5-Methyluridine Methyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-15
Detector	ADSC CCD QUANTUM-4 2X2 ARRAY
Wavelength(s)	0.9786,0.9788
Spacegroup name	P 1 21 1
Unit cell lengths	35.938, 99.448, 58.519
Unit cell angles	90.00, 100.57, 90.00

Refinement procedure

Resolution	50.000 - 1.950
Rwork	0.186
R-free	0.22300 *
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.660 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.094	0.896
Total number of observations	632764 *
Number of reflections	29176
<I/σ(I)>	18.4	2.6
Completeness [%]	100.0	100
Redundancy	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9		PROTEIN WAS CRYSTALLIZED FROM 50 MM TRIS-CL (PH 9-9.5), 10 MM NICL2, 12.5% GLYCEROL, AND 20% POLYETHYLENE GLYCOL 2000 MONOMETHYL ETHER

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	Tris-Cl	50 (mM)	pH9.0-9.5
3	1	reservoir		10 (mM)
4	1	reservoir	glycerol	12.5 (%)
5	1	reservoir	PEG2000 MME	20 (%)