1UR5

Stabilization of a Tetrameric Malate Dehydrogenase by Introduction of a Disulfide Bridge at the Dimer/Dimer Interface

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005737	cellular_component	cytoplasm
A	0006099	biological_process	tricarboxylic acid cycle
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019752	biological_process	carboxylic acid metabolic process
A	0030060	molecular_function	L-malate dehydrogenase (NAD+) activity
C	0003824	molecular_function	catalytic activity
C	0005737	cellular_component	cytoplasm
C	0006099	biological_process	tricarboxylic acid cycle
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019752	biological_process	carboxylic acid metabolic process
C	0030060	molecular_function	L-malate dehydrogenase (NAD+) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A1309

Chain	Residue
A	GLU178
A	CL1312
A	HOH2156
A	HOH2157

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CD A1310

Chain	Residue
A	GLU165
C	HOH2203
C	HOH2211

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A1311

Chain	Residue
A	GLU277
A	HOH2238
A	ASP200
A	ASP243

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A1312

Chain	Residue
A	ASP123
A	ALA124
A	CD1309

---

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE NA A1313

Chain	Residue
A	HIS19

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD C1310

Chain	Residue
C	GLU178
C	HOH2083
C	HOH2113
C	HOH2115

---

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CD C1311

Chain	Residue
A	HOH2212
C	GLU165

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD C1312

Chain	Residue
A	GLU159
A	HOH2139
C	GLU159
C	HOH2140

---

site_id	AC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE NA C1313

Chain	Residue
C	HIS19

---

site_id	BC1
Number of Residues	24
Details	BINDING SITE FOR RESIDUE NAD A1308

Chain	Residue
A	GLY9
A	GLY11
A	PHE12
A	VAL13
A	ASP33
A	ILE34
A	VAL35
A	TYR65
A	THR77
A	SER78
A	GLY79
A	VAL118
A	ASN119
A	ASN120
A	LEU147
A	HIS175
A	ALA225
A	PRO229
A	HOH2277
A	HOH2278
A	HOH2279
A	HOH2280
A	HOH2281
A	HOH2282

---

site_id	BC2
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAD C1309

Chain	Residue
C	GLY9
C	GLY11
C	PHE12
C	VAL13
C	ASP33
C	ILE34
C	VAL35
C	TYR65
C	THR77
C	SER78
C	GLY79
C	ALA80
C	CYS102
C	VAL118
C	ASN120
C	LEU147
C	HIS175
C	PRO229
C	HOH2197
C	HOH2262
C	HOH2263
C	HOH2264
C	HOH2265

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000250|UniProtKB:P61889, ECO:0000255|HAMAP-Rule:MF_00487

Chain	Residue	Details
A	HIS175
C	HIS175

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00487, ECO:0000269|PubMed:12054817, ECO:0000269|PubMed:14636605

Chain	Residue	Details
A	GLY9
A	ASP33
A	VAL118
C	GLY9
C	ASP33
C	VAL118

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P61889, ECO:0000255|HAMAP-Rule:MF_00487

Chain	Residue	Details
A	ARG82
C	ARG151
A	ARG88
A	ASN95
A	ASN120
A	ARG151
C	ARG82
C	ARG88
C	ASN95
C	ASN120

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
A	HIS175
A	ASP148

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
C	HIS175
C	ASP148

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
A	HIS175
A	ASP148
A	ARG151

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1emd

Chain	Residue	Details
C	HIS175
C	ASP148
C	ARG151

---