1UR5

Stabilization of a Tetrameric Malate Dehydrogenase by Introduction of a Disulfide Bridge at the Dimer/Dimer Interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	105.575, 105.575, 102.531
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.750
R-factor	0.19
Rwork	0.190
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1guy
RMSD bond length	0.004 *
RMSD bond angle	21.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.129	0.457
Total number of observations	674394 *
Number of reflections	66684
<I/σ(I)>	22.8	4.6
Completeness [%]	96.6	89
Redundancy	10.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	288 *	15% PEG400, 100MM NAAC, PH 4.5, 40MM CD2+

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG400	15 (%)
3	1	reservoir	sodium acetate	100 (mM)	pH4.5
4	1	reservoir		40 (mM)