Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1TYF

THE STRUCTURE OF CLPP AT 2.3 ANGSTROM RESOLUTION SUGGESTS A MODEL FOR ATP-DEPENDENT PROTEOLYSIS

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004176	molecular_function	ATP-dependent peptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006508	biological_process	proteolysis
A	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
A	0008236	molecular_function	serine-type peptidase activity
A	0009266	biological_process	response to temperature stimulus
A	0009314	biological_process	response to radiation
A	0009368	cellular_component	endopeptidase Clp complex
A	0009376	cellular_component	HslUV protease complex
A	0009408	biological_process	response to heat
A	0010498	biological_process	proteasomal protein catabolic process
A	0016020	cellular_component	membrane
A	0042802	molecular_function	identical protein binding
A	0043068	biological_process	positive regulation of programmed cell death
A	0051117	molecular_function	ATPase binding
B	0004176	molecular_function	ATP-dependent peptidase activity
B	0004252	molecular_function	serine-type endopeptidase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006508	biological_process	proteolysis
B	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
B	0008236	molecular_function	serine-type peptidase activity
B	0009266	biological_process	response to temperature stimulus
B	0009314	biological_process	response to radiation
B	0009368	cellular_component	endopeptidase Clp complex
B	0009376	cellular_component	HslUV protease complex
B	0009408	biological_process	response to heat
B	0010498	biological_process	proteasomal protein catabolic process
B	0016020	cellular_component	membrane
B	0042802	molecular_function	identical protein binding
B	0043068	biological_process	positive regulation of programmed cell death
B	0051117	molecular_function	ATPase binding
C	0004176	molecular_function	ATP-dependent peptidase activity
C	0004252	molecular_function	serine-type endopeptidase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006508	biological_process	proteolysis
C	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
C	0008236	molecular_function	serine-type peptidase activity
C	0009266	biological_process	response to temperature stimulus
C	0009314	biological_process	response to radiation
C	0009368	cellular_component	endopeptidase Clp complex
C	0009376	cellular_component	HslUV protease complex
C	0009408	biological_process	response to heat
C	0010498	biological_process	proteasomal protein catabolic process
C	0016020	cellular_component	membrane
C	0042802	molecular_function	identical protein binding
C	0043068	biological_process	positive regulation of programmed cell death
C	0051117	molecular_function	ATPase binding
D	0004176	molecular_function	ATP-dependent peptidase activity
D	0004252	molecular_function	serine-type endopeptidase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006508	biological_process	proteolysis
D	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
D	0008236	molecular_function	serine-type peptidase activity
D	0009266	biological_process	response to temperature stimulus
D	0009314	biological_process	response to radiation
D	0009368	cellular_component	endopeptidase Clp complex
D	0009376	cellular_component	HslUV protease complex
D	0009408	biological_process	response to heat
D	0010498	biological_process	proteasomal protein catabolic process
D	0016020	cellular_component	membrane
D	0042802	molecular_function	identical protein binding
D	0043068	biological_process	positive regulation of programmed cell death
D	0051117	molecular_function	ATPase binding
E	0004176	molecular_function	ATP-dependent peptidase activity
E	0004252	molecular_function	serine-type endopeptidase activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006508	biological_process	proteolysis
E	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
E	0008236	molecular_function	serine-type peptidase activity
E	0009266	biological_process	response to temperature stimulus
E	0009314	biological_process	response to radiation
E	0009368	cellular_component	endopeptidase Clp complex
E	0009376	cellular_component	HslUV protease complex
E	0009408	biological_process	response to heat
E	0010498	biological_process	proteasomal protein catabolic process
E	0016020	cellular_component	membrane
E	0042802	molecular_function	identical protein binding
E	0043068	biological_process	positive regulation of programmed cell death
E	0051117	molecular_function	ATPase binding
F	0004176	molecular_function	ATP-dependent peptidase activity
F	0004252	molecular_function	serine-type endopeptidase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006508	biological_process	proteolysis
F	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
F	0008236	molecular_function	serine-type peptidase activity
F	0009266	biological_process	response to temperature stimulus
F	0009314	biological_process	response to radiation
F	0009368	cellular_component	endopeptidase Clp complex
F	0009376	cellular_component	HslUV protease complex
F	0009408	biological_process	response to heat
F	0010498	biological_process	proteasomal protein catabolic process
F	0016020	cellular_component	membrane
F	0042802	molecular_function	identical protein binding
F	0043068	biological_process	positive regulation of programmed cell death
F	0051117	molecular_function	ATPase binding
G	0004176	molecular_function	ATP-dependent peptidase activity
G	0004252	molecular_function	serine-type endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006508	biological_process	proteolysis
G	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
G	0008236	molecular_function	serine-type peptidase activity
G	0009266	biological_process	response to temperature stimulus
G	0009314	biological_process	response to radiation
G	0009368	cellular_component	endopeptidase Clp complex
G	0009376	cellular_component	HslUV protease complex
G	0009408	biological_process	response to heat
G	0010498	biological_process	proteasomal protein catabolic process
G	0016020	cellular_component	membrane
G	0042802	molecular_function	identical protein binding
G	0043068	biological_process	positive regulation of programmed cell death
G	0051117	molecular_function	ATPase binding
H	0004176	molecular_function	ATP-dependent peptidase activity
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006508	biological_process	proteolysis
H	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
H	0008236	molecular_function	serine-type peptidase activity
H	0009266	biological_process	response to temperature stimulus
H	0009314	biological_process	response to radiation
H	0009368	cellular_component	endopeptidase Clp complex
H	0009376	cellular_component	HslUV protease complex
H	0009408	biological_process	response to heat
H	0010498	biological_process	proteasomal protein catabolic process
H	0016020	cellular_component	membrane
H	0042802	molecular_function	identical protein binding
H	0043068	biological_process	positive regulation of programmed cell death
H	0051117	molecular_function	ATPase binding
I	0004176	molecular_function	ATP-dependent peptidase activity
I	0004252	molecular_function	serine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006508	biological_process	proteolysis
I	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
I	0008236	molecular_function	serine-type peptidase activity
I	0009266	biological_process	response to temperature stimulus
I	0009314	biological_process	response to radiation
I	0009368	cellular_component	endopeptidase Clp complex
I	0009376	cellular_component	HslUV protease complex
I	0009408	biological_process	response to heat
I	0010498	biological_process	proteasomal protein catabolic process
I	0016020	cellular_component	membrane
I	0042802	molecular_function	identical protein binding
I	0043068	biological_process	positive regulation of programmed cell death
I	0051117	molecular_function	ATPase binding
J	0004176	molecular_function	ATP-dependent peptidase activity
J	0004252	molecular_function	serine-type endopeptidase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006508	biological_process	proteolysis
J	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
J	0008236	molecular_function	serine-type peptidase activity
J	0009266	biological_process	response to temperature stimulus
J	0009314	biological_process	response to radiation
J	0009368	cellular_component	endopeptidase Clp complex
J	0009376	cellular_component	HslUV protease complex
J	0009408	biological_process	response to heat
J	0010498	biological_process	proteasomal protein catabolic process
J	0016020	cellular_component	membrane
J	0042802	molecular_function	identical protein binding
J	0043068	biological_process	positive regulation of programmed cell death
J	0051117	molecular_function	ATPase binding
K	0004176	molecular_function	ATP-dependent peptidase activity
K	0004252	molecular_function	serine-type endopeptidase activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006508	biological_process	proteolysis
K	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
K	0008236	molecular_function	serine-type peptidase activity
K	0009266	biological_process	response to temperature stimulus
K	0009314	biological_process	response to radiation
K	0009368	cellular_component	endopeptidase Clp complex
K	0009376	cellular_component	HslUV protease complex
K	0009408	biological_process	response to heat
K	0010498	biological_process	proteasomal protein catabolic process
K	0016020	cellular_component	membrane
K	0042802	molecular_function	identical protein binding
K	0043068	biological_process	positive regulation of programmed cell death
K	0051117	molecular_function	ATPase binding
L	0004176	molecular_function	ATP-dependent peptidase activity
L	0004252	molecular_function	serine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006508	biological_process	proteolysis
L	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
L	0008236	molecular_function	serine-type peptidase activity
L	0009266	biological_process	response to temperature stimulus
L	0009314	biological_process	response to radiation
L	0009368	cellular_component	endopeptidase Clp complex
L	0009376	cellular_component	HslUV protease complex
L	0009408	biological_process	response to heat
L	0010498	biological_process	proteasomal protein catabolic process
L	0016020	cellular_component	membrane
L	0042802	molecular_function	identical protein binding
L	0043068	biological_process	positive regulation of programmed cell death
L	0051117	molecular_function	ATPase binding
M	0004176	molecular_function	ATP-dependent peptidase activity
M	0004252	molecular_function	serine-type endopeptidase activity
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0006508	biological_process	proteolysis
M	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
M	0008236	molecular_function	serine-type peptidase activity
M	0009266	biological_process	response to temperature stimulus
M	0009314	biological_process	response to radiation
M	0009368	cellular_component	endopeptidase Clp complex
M	0009376	cellular_component	HslUV protease complex
M	0009408	biological_process	response to heat
M	0010498	biological_process	proteasomal protein catabolic process
M	0016020	cellular_component	membrane
M	0042802	molecular_function	identical protein binding
M	0043068	biological_process	positive regulation of programmed cell death
M	0051117	molecular_function	ATPase binding
N	0004176	molecular_function	ATP-dependent peptidase activity
N	0004252	molecular_function	serine-type endopeptidase activity
N	0005515	molecular_function	protein binding
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0006508	biological_process	proteolysis
N	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
N	0008236	molecular_function	serine-type peptidase activity
N	0009266	biological_process	response to temperature stimulus
N	0009314	biological_process	response to radiation
N	0009368	cellular_component	endopeptidase Clp complex
N	0009376	cellular_component	HslUV protease complex
N	0009408	biological_process	response to heat
N	0010498	biological_process	proteasomal protein catabolic process
N	0016020	cellular_component	membrane
N	0042802	molecular_function	identical protein binding
N	0043068	biological_process	positive regulation of programmed cell death
N	0051117	molecular_function	ATPase binding

Functional Information from PROSITE/UniProt

site_id	PS00381
Number of Residues	12
Details	CLP_PROTEASE_SER Endopeptidase Clp serine active site. TicMGqAASMGA

Chain	Residue	Details
A	THR89-ALA100

site_id	PS00382
Number of Residues	14
Details	CLP_PROTEASE_HIS Endopeptidase Clp histidine active site. RfclPnsrVMIHQP

Chain	Residue	Details
A	ARG111-PRO124

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	ACT_SITE: Nucleophile => ECO:0000305

Chain	Residue	Details
A	SER97
J	SER97
K	SER97
L	SER97
M	SER97
N	SER97
B	SER97
C	SER97
D	SER97
E	SER97
F	SER97
G	SER97
H	SER97
I	SER97

site_id	SWS_FT_FI2
Number of Residues	28
Details	ACT_SITE: ACT_SITE => ECO:0000305

Chain	Residue	Details
A	HIS122
E	ASP171
F	HIS122
F	ASP171
G	HIS122
G	ASP171
H	HIS122
H	ASP171
I	HIS122
I	ASP171
J	HIS122
A	ASP171
J	ASP171
K	HIS122
K	ASP171
L	HIS122
L	ASP171
M	HIS122
M	ASP171
N	HIS122
N	ASP171
B	HIS122
B	ASP171
C	HIS122
C	ASP171
D	HIS122
D	ASP171
E	HIS122

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
A	MET98
A	SER97
A	GLY68
A	HIS122
A	ASP171

site_id	CSA10
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
J	MET98
J	SER97
J	GLY68
J	HIS122
J	ASP171

site_id	CSA11
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
K	MET98
K	SER97
K	GLY68
K	HIS122
K	ASP171

site_id	CSA12
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
L	MET98
L	SER97
L	GLY68
L	HIS122
L	ASP171

site_id	CSA13
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
M	MET98
M	SER97
M	GLY68
M	HIS122
M	ASP171

site_id	CSA14
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
N	MET98
N	SER97
N	GLY68
N	HIS122
N	ASP171

site_id	CSA2
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
B	MET98
B	SER97
B	GLY68
B	HIS122
B	ASP171

site_id	CSA3
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
C	MET98
C	SER97
C	GLY68
C	HIS122
C	ASP171

site_id	CSA4
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
D	MET98
D	SER97
D	GLY68
D	HIS122
D	ASP171

site_id	CSA5
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
E	MET98
E	SER97
E	GLY68
E	HIS122
E	ASP171

site_id	CSA6
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
F	MET98
F	SER97
F	GLY68
F	HIS122
F	ASP171

site_id	CSA7
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
G	MET98
G	SER97
G	GLY68
G	HIS122
G	ASP171

site_id	CSA8
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
H	MET98
H	SER97
H	GLY68
H	HIS122
H	ASP171

site_id	CSA9
Number of Residues	5
Details	a catalytic site defined by CSA, PubMed 9390554, 10049803, 10320569

Chain	Residue	Details
I	MET98
I	SER97
I	GLY68
I	HIS122
I	ASP171

site_id	MCSA1
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
A	GLY68	electrostatic stabiliser
A	SER97	covalent catalysis, proton shuttle (general acid/base)
A	MET98	electrostatic stabiliser
A	HIS122	proton shuttle (general acid/base)
A	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA10
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
J	GLY68	electrostatic stabiliser
J	SER97	covalent catalysis, proton shuttle (general acid/base)
J	MET98	electrostatic stabiliser
J	HIS122	proton shuttle (general acid/base)
J	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA11
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
K	GLY68	electrostatic stabiliser
K	SER97	covalent catalysis, proton shuttle (general acid/base)
K	MET98	electrostatic stabiliser
K	HIS122	proton shuttle (general acid/base)
K	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA12
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
L	GLY68	electrostatic stabiliser
L	SER97	covalent catalysis, proton shuttle (general acid/base)
L	MET98	electrostatic stabiliser
L	HIS122	proton shuttle (general acid/base)
L	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA13
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
M	GLY68	electrostatic stabiliser
M	SER97	covalent catalysis, proton shuttle (general acid/base)
M	MET98	electrostatic stabiliser
M	HIS122	proton shuttle (general acid/base)
M	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA14
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
N	GLY68	electrostatic stabiliser
N	SER97	covalent catalysis, proton shuttle (general acid/base)
N	MET98	electrostatic stabiliser
N	HIS122	proton shuttle (general acid/base)
N	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA2
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
B	GLY68	electrostatic stabiliser
B	SER97	covalent catalysis, proton shuttle (general acid/base)
B	MET98	electrostatic stabiliser
B	HIS122	proton shuttle (general acid/base)
B	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA3
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
C	GLY68	electrostatic stabiliser
C	SER97	covalent catalysis, proton shuttle (general acid/base)
C	MET98	electrostatic stabiliser
C	HIS122	proton shuttle (general acid/base)
C	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA4
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
D	GLY68	electrostatic stabiliser
D	SER97	covalent catalysis, proton shuttle (general acid/base)
D	MET98	electrostatic stabiliser
D	HIS122	proton shuttle (general acid/base)
D	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA5
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
E	GLY68	electrostatic stabiliser
E	SER97	covalent catalysis, proton shuttle (general acid/base)
E	MET98	electrostatic stabiliser
E	HIS122	proton shuttle (general acid/base)
E	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA6
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
F	GLY68	electrostatic stabiliser
F	SER97	covalent catalysis, proton shuttle (general acid/base)
F	MET98	electrostatic stabiliser
F	HIS122	proton shuttle (general acid/base)
F	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA7
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
G	GLY68	electrostatic stabiliser
G	SER97	covalent catalysis, proton shuttle (general acid/base)
G	MET98	electrostatic stabiliser
G	HIS122	proton shuttle (general acid/base)
G	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA8
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
H	GLY68	electrostatic stabiliser
H	SER97	covalent catalysis, proton shuttle (general acid/base)
H	MET98	electrostatic stabiliser
H	HIS122	proton shuttle (general acid/base)
H	ASP171	electrostatic stabiliser, modifies pKa

site_id	MCSA9
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
I	GLY68	electrostatic stabiliser
I	SER97	covalent catalysis, proton shuttle (general acid/base)
I	MET98	electrostatic stabiliser
I	HIS122	proton shuttle (general acid/base)
I	ASP171	electrostatic stabiliser, modifies pKa

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)