1TYF
THE STRUCTURE OF CLPP AT 2.3 ANGSTROM RESOLUTION SUGGESTS A MODEL FOR ATP-DEPENDENT PROTEOLYSIS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1995-12 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.320, 102.460, 157.080 |
| Unit cell angles | 90.00, 97.80, 90.00 |
Refinement procedure
| Resolution | 80.000 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.29200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.530 |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | HKL ((SCALEPACK)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.000 * |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.077 |
| Number of reflections | 142952 * |
| Completeness [%] | 100.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.4 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | Na citrate | 100 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
| 7 | 1 | reservoir | isopropanol | 20 (%(v/v)) |
