1T2F
Human B lactate dehydrogenase complexed with NAD+ and 4-hydroxy-1,2,5-oxadiazole-3-carboxylic acid
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004457 | molecular_function | lactate dehydrogenase activity |
| A | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005743 | cellular_component | mitochondrial inner membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0006089 | biological_process | lactate metabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019674 | biological_process | NAD+ metabolic process |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| A | 0019900 | molecular_function | kinase binding |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0042867 | biological_process | pyruvate catabolic process |
| A | 0045121 | cellular_component | membrane raft |
| A | 0051287 | molecular_function | NAD binding |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 1990204 | cellular_component | oxidoreductase complex |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004457 | molecular_function | lactate dehydrogenase activity |
| B | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005743 | cellular_component | mitochondrial inner membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0006089 | biological_process | lactate metabolic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0019674 | biological_process | NAD+ metabolic process |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0019900 | molecular_function | kinase binding |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0042867 | biological_process | pyruvate catabolic process |
| B | 0045121 | cellular_component | membrane raft |
| B | 0051287 | molecular_function | NAD binding |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 1990204 | cellular_component | oxidoreductase complex |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004457 | molecular_function | lactate dehydrogenase activity |
| C | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005743 | cellular_component | mitochondrial inner membrane |
| C | 0005829 | cellular_component | cytosol |
| C | 0006089 | biological_process | lactate metabolic process |
| C | 0016020 | cellular_component | membrane |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0019674 | biological_process | NAD+ metabolic process |
| C | 0019752 | biological_process | carboxylic acid metabolic process |
| C | 0019900 | molecular_function | kinase binding |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0042867 | biological_process | pyruvate catabolic process |
| C | 0045121 | cellular_component | membrane raft |
| C | 0051287 | molecular_function | NAD binding |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 1990204 | cellular_component | oxidoreductase complex |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004457 | molecular_function | lactate dehydrogenase activity |
| D | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005739 | cellular_component | mitochondrion |
| D | 0005743 | cellular_component | mitochondrial inner membrane |
| D | 0005829 | cellular_component | cytosol |
| D | 0006089 | biological_process | lactate metabolic process |
| D | 0016020 | cellular_component | membrane |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0019674 | biological_process | NAD+ metabolic process |
| D | 0019752 | biological_process | carboxylic acid metabolic process |
| D | 0019900 | molecular_function | kinase binding |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0042867 | biological_process | pyruvate catabolic process |
| D | 0045121 | cellular_component | membrane raft |
| D | 0051287 | molecular_function | NAD binding |
| D | 0070062 | cellular_component | extracellular exosome |
| D | 1990204 | cellular_component | oxidoreductase complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE NAD A 401 |
| Chain | Residue |
| A | GLY29 |
| A | VAL98 |
| A | ARG99 |
| A | VAL116 |
| A | ILE120 |
| A | VAL136 |
| A | SER137 |
| A | ASN138 |
| A | SER161 |
| A | HIS193 |
| A | THR248 |
| A | GLN30 |
| A | ILE252 |
| A | OXQ402 |
| A | HOH433 |
| A | HOH434 |
| A | VAL31 |
| A | ASP52 |
| A | VAL53 |
| A | LEU54 |
| A | TYR83 |
| A | THR95 |
| A | GLY97 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE OXQ A 402 |
| Chain | Residue |
| A | GLN100 |
| A | ARG106 |
| A | ASN138 |
| A | LEU165 |
| A | ARG169 |
| A | HIS193 |
| A | THR248 |
| A | NAD401 |
| site_id | AC3 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE NAD B 403 |
| Chain | Residue |
| B | VAL28 |
| B | GLY29 |
| B | GLN30 |
| B | VAL31 |
| B | ASP52 |
| B | VAL53 |
| B | LEU54 |
| B | THR95 |
| B | ALA96 |
| B | GLY97 |
| B | VAL98 |
| B | ARG99 |
| B | GLN100 |
| B | ILE120 |
| B | VAL136 |
| B | SER137 |
| B | ASN138 |
| B | SER161 |
| B | HIS193 |
| B | THR248 |
| B | ILE252 |
| B | OXQ404 |
| B | HOH424 |
| B | HOH473 |
| B | HOH480 |
| D | ASN108 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE OXQ B 404 |
| Chain | Residue |
| B | GLN100 |
| B | ARG106 |
| B | ASN138 |
| B | ARG169 |
| B | HIS193 |
| B | THR248 |
| B | NAD403 |
| B | HOH480 |
| site_id | AC5 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE NAD C 405 |
| Chain | Residue |
| A | ASN108 |
| C | VAL28 |
| C | GLY29 |
| C | GLN30 |
| C | VAL31 |
| C | ASP52 |
| C | VAL53 |
| C | LEU54 |
| C | THR95 |
| C | ALA96 |
| C | GLY97 |
| C | VAL98 |
| C | ARG99 |
| C | VAL116 |
| C | VAL136 |
| C | ASN138 |
| C | SER161 |
| C | HIS193 |
| C | THR248 |
| C | ILE252 |
| C | OXQ406 |
| C | HOH419 |
| C | HOH427 |
| C | HOH437 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE OXQ C 406 |
| Chain | Residue |
| C | GLN100 |
| C | ARG106 |
| C | ASN138 |
| C | LEU165 |
| C | ARG169 |
| C | HIS193 |
| C | ALA238 |
| C | THR248 |
| C | NAD405 |
| C | HOH419 |
| site_id | AC7 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE NAD D 407 |
| Chain | Residue |
| D | GLY29 |
| D | GLN30 |
| D | VAL31 |
| D | ASP52 |
| D | VAL53 |
| D | LEU54 |
| D | THR95 |
| D | ALA96 |
| D | GLY97 |
| D | VAL98 |
| D | ARG99 |
| D | VAL136 |
| D | ASN138 |
| D | SER161 |
| D | LEU165 |
| D | HIS193 |
| D | THR248 |
| D | ILE252 |
| D | OXQ408 |
| D | HOH419 |
| D | HOH420 |
| D | HOH443 |
| D | HOH444 |
| site_id | AC8 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE OXQ D 408 |
| Chain | Residue |
| D | GLN100 |
| D | ARG106 |
| D | ASN138 |
| D | LEU165 |
| D | ARG169 |
| D | HIS193 |
| D | ALA238 |
| D | THR248 |
| D | NAD407 |
| D | HOH419 |
Functional Information from PROSITE/UniProt
| site_id | PS00064 |
| Number of Residues | 7 |
| Details | L_LDH L-lactate dehydrogenase active site. LGEHGDS |
| Chain | Residue | Details |
| A | LEU190-SER196 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| A | GLY194 | |
| B | GLY194 | |
| C | GLY194 | |
| D | GLY194 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | VAL31 | |
| B | VAL31 | |
| C | VAL31 | |
| D | VAL31 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11276087 |
| Chain | Residue | Details |
| A | GLN100 | |
| B | GLN100 | |
| C | GLN100 | |
| D | GLN100 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 16 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | LEU107 | |
| C | PRO139 | |
| C | PHE170 | |
| C | ASN249 | |
| D | LEU107 | |
| D | PRO139 | |
| D | PHE170 | |
| D | ASN249 | |
| A | PRO139 | |
| A | PHE170 | |
| A | ASN249 | |
| B | LEU107 | |
| B | PRO139 | |
| B | PHE170 | |
| B | ASN249 | |
| C | LEU107 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | MOD_RES: N-acetylalanine => ECO:0000269|Ref.4 |
| Chain | Residue | Details |
| A | THR2 | |
| B | THR2 | |
| C | THR2 | |
| D | THR2 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 16 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LEU7 | |
| C | LEU58 | |
| C | PHE119 | |
| C | ASP329 | |
| D | LEU7 | |
| D | LEU58 | |
| D | PHE119 | |
| D | ASP329 | |
| A | LEU58 | |
| A | PHE119 | |
| A | ASP329 | |
| B | LEU7 | |
| B | LEU58 | |
| B | PHE119 | |
| B | ASP329 | |
| C | LEU7 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332 |
| Chain | Residue | Details |
| A | LEU44 | |
| B | LEU44 | |
| C | LEU44 | |
| D | LEU44 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231 |
| Chain | Residue | Details |
| A | GLU240 | |
| B | GLU240 | |
| C | GLU240 | |
| D | GLU240 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| A | ASP166 | |
| A | HIS193 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| B | ASP166 | |
| B | HIS193 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| C | ASP166 | |
| C | HIS193 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| D | ASP166 | |
| D | HIS193 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| A | HIS193 | |
| A | ASP166 | |
| A | ARG169 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| B | HIS193 | |
| B | ASP166 | |
| B | ARG169 |
| site_id | CSA7 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| C | HIS193 | |
| C | ASP166 | |
| C | ARG169 |
| site_id | CSA8 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1emd |
| Chain | Residue | Details |
| D | HIS193 | |
| D | ASP166 | |
| D | ARG169 |
