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STRUCTURE OF HOLO-GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE FROM PALINURUS VERSICOLOR REFINED 2.0 ANGSTROM RESOLUTION

Functional Information from GO Data

Chain	GOid	namespace	contents
G	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006006	biological_process	glucose metabolic process
G	0006096	biological_process	glycolytic process
G	0016491	molecular_function	oxidoreductase activity
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0050661	molecular_function	NADP binding
G	0051287	molecular_function	NAD binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006006	biological_process	glucose metabolic process
R	0006096	biological_process	glycolytic process
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 G 501

Chain	Residue
G	SER148
G	THR208
G	GLY209
G	ALA210
G	HOH541
G	HOH600
G	HOH671

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site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 G 502

Chain	Residue
G	THR179
G	THR181
G	ARG231
G	NAD335
G	HOH546

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 R 501

Chain	Residue
R	SER148
R	THR208
R	GLY209
R	ALA210
R	HOH616

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 R 502

Chain	Residue
R	THR179
R	THR181
R	ARG231
R	NAD335

---

site_id	AC5
Number of Residues	30
Details	BINDING SITE FOR RESIDUE NAD G 335

Chain	Residue
G	ASN6
G	GLY7
G	PHE8
G	GLY9
G	ARG10
G	ILE11
G	ASN31
G	ASP32
G	PRO33
G	PHE34
G	MET77
G	SER95
G	THR96
G	GLY97
G	SER119
G	ALA120
G	CYS149
G	ALA180
G	ASN313
G	TYR317
G	SO4502
G	HOH505
G	HOH513
G	HOH514
G	HOH522
G	HOH577
G	HOH601
G	HOH619
G	HOH636
G	HOH688

---

site_id	AC6
Number of Residues	24
Details	BINDING SITE FOR RESIDUE NAD R 335

Chain	Residue
R	ASN6
R	GLY7
R	GLY9
R	ARG10
R	ILE11
R	ASN31
R	ASP32
R	PRO33
R	PHE34
R	MET77
R	SER95
R	THR96
R	GLY97
R	SER119
R	ALA120
R	CYS149
R	ASN313
R	SO4502
R	HOH507
R	HOH508
R	HOH522
R	HOH595
R	HOH596
R	HOH597

---

site_id	ACT
Number of Residues	2
Details	ACTIVE SITE RESIDUES CYS 149 AND HIS 176

Chain	Residue
G	CYS149
G	HIS176

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Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
G	ALA147-LEU154

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
G	CYS149
R	CYS149

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:9761850

Chain	Residue	Details
G	ARG10
G	ASP32
G	ASN313
R	ARG10
R	ASP32
R	ASN313

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
G	MET77
R	ARG231
G	SER148
G	THR179
G	THR208
G	ARG231
R	MET77
R	SER148
R	THR179
R	THR208

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Activates thiol group during catalysis

Chain	Residue	Details
G	HIS176
R	HIS176

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N-acetylserine => ECO:0000250|UniProtKB:P00357

Chain	Residue	Details
G	SER1
R	SER1

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 9144771

Chain	Residue	Details
G	CYS149
G	HIS176

---

site_id	CSA2
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 9144771

Chain	Residue	Details
R	CYS149
R	HIS176

---

site_id	MCSA1
Number of Residues	2
Details	M-CSA 911

Chain	Residue	Details
G	CYS149	covalent catalysis, proton shuttle (general acid/base)
G	HIS176	proton shuttle (general acid/base)

---

site_id	MCSA2
Number of Residues	2
Details	M-CSA 911

Chain	Residue	Details
R	CYS149	covalent catalysis, proton shuttle (general acid/base)
R	HIS176	proton shuttle (general acid/base)

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