1SZJ

STRUCTURE OF HOLO-GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE FROM PALINURUS VERSICOLOR REFINED 2.0 ANGSTROM RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	280
Detector technology	DIFFRACTOMETER
Collection date	1994-05
Detector	WEISSENBERG
Spacegroup name	C 1 2 1
Unit cell lengths	128.400, 99.900, 80.790
Unit cell angles	90.00, 114.48, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.168
Rwork	0.168
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOMARUS AMERICANUS HOLO-GAPDH
RMSD bond length	0.014
RMSD bond angle	2.850
Data reduction software	WEIS
Data scaling software	CCP4
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.069	0.069
Number of reflections	84900
<I/σ(I)>	3.9	1.6
Completeness [%]	83.5	56.5
Redundancy	1.4	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.1	280	THE PROTEIN SOLUTIONS CONTAINED 0.5MM NAD+, 1.0MM EDTA, 1.6M AMMONIUM SULPHATE IN 0.1M PHOSPHATE BUFFER (PH 6.1) AND AN ENZYME CONCENTRATION OF 8MG/ML; THE SOLUTION IN RESERVOIR CONTAINED 2.7M AMMONIUM SULPHATE IN SAME BUFFER. THE CRYSTALLIZATION WAS CARRIED OUT AT 280K.