1S4D
Crystal Structure Analysis of the S-adenosyl-L-methionine dependent uroporphyrinogen-III C-methyltransferase SUMT
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| A | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009236 | biological_process | cobalamin biosynthetic process |
| A | 0019354 | biological_process | siroheme biosynthetic process |
| A | 0032259 | biological_process | methylation |
| B | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| B | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009236 | biological_process | cobalamin biosynthetic process |
| B | 0019354 | biological_process | siroheme biosynthetic process |
| B | 0032259 | biological_process | methylation |
| D | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| D | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| D | 0008168 | molecular_function | methyltransferase activity |
| D | 0009236 | biological_process | cobalamin biosynthetic process |
| D | 0019354 | biological_process | siroheme biosynthetic process |
| D | 0032259 | biological_process | methylation |
| E | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| E | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| E | 0008168 | molecular_function | methyltransferase activity |
| E | 0009236 | biological_process | cobalamin biosynthetic process |
| E | 0019354 | biological_process | siroheme biosynthetic process |
| E | 0032259 | biological_process | methylation |
| F | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| F | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| F | 0008168 | molecular_function | methyltransferase activity |
| F | 0009236 | biological_process | cobalamin biosynthetic process |
| F | 0019354 | biological_process | siroheme biosynthetic process |
| F | 0032259 | biological_process | methylation |
| G | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| G | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| G | 0008168 | molecular_function | methyltransferase activity |
| G | 0009236 | biological_process | cobalamin biosynthetic process |
| G | 0019354 | biological_process | siroheme biosynthetic process |
| G | 0032259 | biological_process | methylation |
| H | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| H | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| H | 0008168 | molecular_function | methyltransferase activity |
| H | 0009236 | biological_process | cobalamin biosynthetic process |
| H | 0019354 | biological_process | siroheme biosynthetic process |
| H | 0032259 | biological_process | methylation |
| I | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| I | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| I | 0008168 | molecular_function | methyltransferase activity |
| I | 0009236 | biological_process | cobalamin biosynthetic process |
| I | 0019354 | biological_process | siroheme biosynthetic process |
| I | 0032259 | biological_process | methylation |
| J | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| J | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| J | 0008168 | molecular_function | methyltransferase activity |
| J | 0009236 | biological_process | cobalamin biosynthetic process |
| J | 0019354 | biological_process | siroheme biosynthetic process |
| J | 0032259 | biological_process | methylation |
| K | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| K | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| K | 0008168 | molecular_function | methyltransferase activity |
| K | 0009236 | biological_process | cobalamin biosynthetic process |
| K | 0019354 | biological_process | siroheme biosynthetic process |
| K | 0032259 | biological_process | methylation |
| L | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| L | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| L | 0008168 | molecular_function | methyltransferase activity |
| L | 0009236 | biological_process | cobalamin biosynthetic process |
| L | 0019354 | biological_process | siroheme biosynthetic process |
| L | 0032259 | biological_process | methylation |
| M | 0004851 | molecular_function | uroporphyrin-III C-methyltransferase activity |
| M | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| M | 0008168 | molecular_function | methyltransferase activity |
| M | 0009236 | biological_process | cobalamin biosynthetic process |
| M | 0019354 | biological_process | siroheme biosynthetic process |
| M | 0032259 | biological_process | methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE SAH A 1501 |
| Chain | Residue |
| A | PRO24 |
| A | MET184 |
| A | VAL210 |
| A | ASN212 |
| A | ALA213 |
| A | PRO240 |
| A | ALA241 |
| A | ILE242 |
| A | HOH1546 |
| A | GLY100 |
| A | GLY101 |
| A | ASP102 |
| A | VAL105 |
| A | PHE106 |
| A | THR130 |
| A | ALA131 |
| A | TYR183 |
| site_id | AC2 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE SAH B 1511 |
| Chain | Residue |
| B | PRO24 |
| B | LEU49 |
| B | GLY100 |
| B | GLY101 |
| B | ASP102 |
| B | VAL105 |
| B | PHE106 |
| B | THR130 |
| B | ALA131 |
| B | TYR183 |
| B | MET184 |
| B | VAL210 |
| B | ASN212 |
| B | ALA213 |
| B | PRO240 |
| B | ALA241 |
| B | ILE242 |
| B | HOH1517 |
| site_id | AC3 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE SAH D 2501 |
| Chain | Residue |
| D | PRO24 |
| D | GLY100 |
| D | GLY101 |
| D | ASP102 |
| D | VAL105 |
| D | PHE106 |
| D | THR130 |
| D | ALA131 |
| D | TYR183 |
| D | MET184 |
| D | VAL210 |
| D | ASN212 |
| D | ALA213 |
| D | PRO240 |
| D | ALA241 |
| D | ILE242 |
| D | HOH2504 |
| D | HOH2505 |
| D | HOH2513 |
| D | HOH2537 |
| site_id | AC4 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE SAH E 2511 |
| Chain | Residue |
| E | PRO24 |
| E | GLY100 |
| E | GLY101 |
| E | ASP102 |
| E | VAL105 |
| E | PHE106 |
| E | THR130 |
| E | ALA131 |
| E | TYR183 |
| E | MET184 |
| E | VAL210 |
| E | ASN212 |
| E | ALA213 |
| E | PRO240 |
| E | ALA241 |
| E | ILE242 |
| E | HOH2526 |
| E | HOH2531 |
| site_id | AC5 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE SAH F 3501 |
| Chain | Residue |
| F | PRO24 |
| F | GLY100 |
| F | GLY101 |
| F | ASP102 |
| F | VAL105 |
| F | PHE106 |
| F | THR130 |
| F | ALA131 |
| F | TYR183 |
| F | MET184 |
| F | VAL210 |
| F | ASN212 |
| F | ALA213 |
| F | PRO240 |
| F | ALA241 |
| F | ILE242 |
| F | HOH3511 |
| site_id | AC6 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE SAH G 3511 |
| Chain | Residue |
| G | MET184 |
| G | VAL210 |
| G | ASN212 |
| G | ALA213 |
| G | PRO240 |
| G | ALA241 |
| G | ILE242 |
| G | HOH3522 |
| G | PRO24 |
| G | GLY100 |
| G | GLY101 |
| G | ASP102 |
| G | VAL105 |
| G | PHE106 |
| G | THR130 |
| G | ALA131 |
| G | TYR183 |
| site_id | AC7 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE SAH H 4501 |
| Chain | Residue |
| H | PRO24 |
| H | GLY100 |
| H | GLY101 |
| H | ASP102 |
| H | VAL105 |
| H | PHE106 |
| H | THR130 |
| H | ALA131 |
| H | TYR183 |
| H | MET184 |
| H | VAL210 |
| H | ASN212 |
| H | ALA213 |
| H | PRO240 |
| H | ALA241 |
| H | ILE242 |
| H | HOH4503 |
| H | HOH4524 |
| site_id | AC8 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE SAH I 4511 |
| Chain | Residue |
| I | PRO24 |
| I | LEU49 |
| I | GLY100 |
| I | GLY101 |
| I | ASP102 |
| I | VAL105 |
| I | PHE106 |
| I | THR130 |
| I | ALA131 |
| I | TYR183 |
| I | MET184 |
| I | VAL210 |
| I | ASN212 |
| I | ALA213 |
| I | ALA241 |
| I | ILE242 |
| site_id | AC9 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE SAH J 5501 |
| Chain | Residue |
| J | PRO24 |
| J | GLY100 |
| J | GLY101 |
| J | ASP102 |
| J | VAL105 |
| J | PHE106 |
| J | THR130 |
| J | ALA131 |
| J | TYR183 |
| J | MET184 |
| J | VAL210 |
| J | ASN212 |
| J | ALA213 |
| J | PRO240 |
| J | ALA241 |
| J | ILE242 |
| J | HOH5508 |
| J | HOH5511 |
| site_id | BC1 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE SAH K 5511 |
| Chain | Residue |
| K | PRO24 |
| K | GLY100 |
| K | GLY101 |
| K | ASP102 |
| K | VAL105 |
| K | PHE106 |
| K | THR130 |
| K | ALA131 |
| K | TYR183 |
| K | MET184 |
| K | VAL210 |
| K | ASN212 |
| K | ALA213 |
| K | PRO240 |
| K | ALA241 |
| K | ILE242 |
| K | HOH5526 |
| site_id | BC2 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE SAH L 6501 |
| Chain | Residue |
| L | PRO24 |
| L | GLY100 |
| L | GLY101 |
| L | ASP102 |
| L | VAL105 |
| L | PHE106 |
| L | THR130 |
| L | ALA131 |
| L | TYR183 |
| L | MET184 |
| L | VAL210 |
| L | ASN212 |
| L | ALA213 |
| L | PRO240 |
| L | ALA241 |
| L | ILE242 |
| L | HOH6505 |
| site_id | BC3 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE SAH M 6511 |
| Chain | Residue |
| M | PRO24 |
| M | GLY100 |
| M | GLY101 |
| M | ASP102 |
| M | VAL105 |
| M | PHE106 |
| M | THR130 |
| M | ALA131 |
| M | TYR183 |
| M | MET184 |
| M | VAL210 |
| M | ASN212 |
| M | ALA213 |
| M | PRO240 |
| M | ALA241 |
| M | ILE242 |
| site_id | BC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL A 1001 |
| Chain | Residue |
| A | GLY128 |
| A | ILE129 |
| A | THR130 |
| A | ILE133 |
| B | ASP102 |
| B | GLY128 |
| B | ILE129 |
| B | THR130 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE GOL A 1002 |
| Chain | Residue |
| A | MET184 |
| A | ALA185 |
| A | MET186 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL B 1011 |
| Chain | Residue |
| B | HIS158 |
| B | MET184 |
| B | ALA185 |
| B | MET186 |
| B | LYS187 |
| site_id | BC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 1012 |
| Chain | Residue |
| A | THR144 |
| A | HIS145 |
| A | ARG146 |
| B | PHE106 |
| B | ARG108 |
| B | GLU111 |
| site_id | BC8 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GOL E 2001 |
| Chain | Residue |
| D | ASP102 |
| D | PRO127 |
| D | GLY128 |
| D | ILE129 |
| D | THR130 |
| E | GLY128 |
| E | ILE129 |
| E | THR130 |
| E | ILE133 |
| E | HOH2545 |
| site_id | BC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE GOL D 2002 |
| Chain | Residue |
| D | MET184 |
| D | MET186 |
| D | LYS187 |
| site_id | CC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL E 2003 |
| Chain | Residue |
| D | PHE106 |
| D | GLY107 |
| D | ARG108 |
| D | GLY110 |
| D | GLU111 |
| E | THR144 |
| E | HIS145 |
| E | ARG146 |
| site_id | CC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL D 2011 |
| Chain | Residue |
| D | HIS145 |
| D | ARG146 |
| E | PHE106 |
| E | ARG108 |
| E | GLY110 |
| E | GLU111 |
| site_id | CC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL E 2012 |
| Chain | Residue |
| E | HIS158 |
| E | MET184 |
| E | MET186 |
| E | LYS187 |
| site_id | CC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL F 3001 |
| Chain | Residue |
| F | HIS158 |
| F | MET184 |
| F | MET186 |
| F | LYS187 |
| site_id | CC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL G 3002 |
| Chain | Residue |
| F | ASP102 |
| F | GLY128 |
| F | THR130 |
| G | GLY128 |
| G | ILE129 |
| G | THR130 |
| G | ILE133 |
| G | GLY134 |
| G | HOH3516 |
| site_id | CC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL G 3003 |
| Chain | Residue |
| F | PHE106 |
| F | GLY107 |
| F | ARG108 |
| F | GLU111 |
| G | THR144 |
| G | HIS145 |
| G | ARG146 |
| site_id | CC7 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL F 3011 |
| Chain | Residue |
| F | HIS145 |
| F | ARG146 |
| F | ASN149 |
| G | PHE106 |
| G | GLY107 |
| G | ARG108 |
| G | GLU111 |
| site_id | CC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL G 3012 |
| Chain | Residue |
| G | HIS158 |
| G | MET184 |
| G | ALA185 |
| G | MET186 |
| G | LYS187 |
| site_id | CC9 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GOL I 4002 |
| Chain | Residue |
| H | ASP102 |
| H | PRO127 |
| H | GLY128 |
| H | THR130 |
| I | GLY128 |
| I | ILE129 |
| I | THR130 |
| I | ILE133 |
| I | GLY134 |
| I | HOH4541 |
| site_id | DC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL H 4003 |
| Chain | Residue |
| H | MET184 |
| H | ALA185 |
| H | MET186 |
| H | LYS187 |
| site_id | DC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL I 4011 |
| Chain | Residue |
| I | HIS158 |
| I | MET184 |
| I | ALA185 |
| I | MET186 |
| I | LYS187 |
| site_id | DC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL H 4012 |
| Chain | Residue |
| H | THR144 |
| H | HIS145 |
| H | ARG146 |
| I | PHE106 |
| I | ARG108 |
| I | GLY110 |
| I | GLU111 |
| site_id | DC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL K 5001 |
| Chain | Residue |
| J | PHE106 |
| J | GLY107 |
| J | ARG108 |
| J | GLU111 |
| K | THR144 |
| K | HIS145 |
| K | ARG146 |
| K | HOH5521 |
| site_id | DC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL J 5002 |
| Chain | Residue |
| J | ASP102 |
| J | PRO127 |
| J | GLY128 |
| J | ILE129 |
| J | THR130 |
| K | GLY128 |
| K | ILE129 |
| K | THR130 |
| K | ILE133 |
| site_id | DC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL J 5003 |
| Chain | Residue |
| J | MET184 |
| J | ALA185 |
| J | MET186 |
| J | LYS187 |
| J | PRO239 |
| J | HOH5508 |
| site_id | DC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL J 5011 |
| Chain | Residue |
| J | THR144 |
| J | HIS145 |
| J | ARG146 |
| J | HOH5514 |
| K | PHE106 |
| K | ARG108 |
| site_id | DC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL K 5012 |
| Chain | Residue |
| K | MET184 |
| K | ALA185 |
| K | MET186 |
| K | HOH5530 |
| K | HOH5541 |
| K | HOH5552 |
| site_id | DC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL L 6001 |
| Chain | Residue |
| L | GLY128 |
| L | ILE129 |
| L | THR130 |
| L | ILE133 |
| M | ASP102 |
| M | PRO127 |
| M | GLY128 |
| M | ILE129 |
| M | THR130 |
| site_id | EC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL L 6002 |
| Chain | Residue |
| L | HIS158 |
| L | MET184 |
| L | ALA185 |
| L | MET186 |
| L | HOH6511 |
| site_id | EC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL M 6011 |
| Chain | Residue |
| M | MET184 |
| M | ALA185 |
| M | MET186 |
| M | LYS187 |
| M | PRO239 |
Functional Information from PROSITE/UniProt
| site_id | PS00839 |
| Number of Residues | 15 |
| Details | SUMT_1 Uroporphyrin-III C-methyltransferase signature 1. VGAGPGdpgLLTLHA |
| Chain | Residue | Details |
| A | VAL20-ALA34 |
| site_id | PS00840 |
| Number of Residues | 34 |
| Details | SUMT_2 Uroporphyrin-III C-methyltransferase signature 2. VlrLkgGDpfvFGrggeealtLvehqvpFrIvPG |
| Chain | Residue | Details |
| A | VAL95-GLY128 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 72 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15522295, ECO:0007744|PDB:1S4D |
| Chain | Residue | Details |
| A | PRO24 | |
| B | MET184 | |
| B | ALA213 | |
| B | ALA241 | |
| D | PRO24 | |
| D | GLY100 | |
| D | THR130 | |
| D | MET184 | |
| D | ALA213 | |
| D | ALA241 | |
| E | PRO24 | |
| A | GLY100 | |
| E | GLY100 | |
| E | THR130 | |
| E | MET184 | |
| E | ALA213 | |
| E | ALA241 | |
| F | PRO24 | |
| F | GLY100 | |
| F | THR130 | |
| F | MET184 | |
| F | ALA213 | |
| A | THR130 | |
| F | ALA241 | |
| G | PRO24 | |
| G | GLY100 | |
| G | THR130 | |
| G | MET184 | |
| G | ALA213 | |
| G | ALA241 | |
| H | PRO24 | |
| H | GLY100 | |
| H | THR130 | |
| A | MET184 | |
| H | MET184 | |
| H | ALA213 | |
| H | ALA241 | |
| I | PRO24 | |
| I | GLY100 | |
| I | THR130 | |
| I | MET184 | |
| I | ALA213 | |
| I | ALA241 | |
| J | PRO24 | |
| A | ALA213 | |
| J | GLY100 | |
| J | THR130 | |
| J | MET184 | |
| J | ALA213 | |
| J | ALA241 | |
| K | PRO24 | |
| K | GLY100 | |
| K | THR130 | |
| K | MET184 | |
| K | ALA213 | |
| A | ALA241 | |
| K | ALA241 | |
| L | PRO24 | |
| L | GLY100 | |
| L | THR130 | |
| L | MET184 | |
| L | ALA213 | |
| L | ALA241 | |
| M | PRO24 | |
| M | GLY100 | |
| M | THR130 | |
| B | PRO24 | |
| M | MET184 | |
| M | ALA213 | |
| M | ALA241 | |
| B | GLY100 | |
| B | THR130 |
