1S4D
Crystal Structure Analysis of the S-adenosyl-L-methionine dependent uroporphyrinogen-III C-methyltransferase SUMT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 4 |
| Unit cell lengths | 218.097, 218.097, 190.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 2.700 |
| R-factor | 0.21526 |
| Rwork | 0.213 |
| R-free | 0.26032 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pjq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.744 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.057 | 0.416 |
| Number of reflections | 121731 | |
| <I/σ(I)> | 19.52 | 3.27 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | PEG 20000, sodium citrate, lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
