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1S26

Structure of Anthrax Edema Factor-Calmodulin-alpha,beta-methyleneadenosine 5'-triphosphate Complex Reveals an Alternative Mode of ATP Binding to the Catalytic Site

Functional Information from GO Data
ChainGOidnamespacecontents
A0005576cellular_componentextracellular region
A0008294molecular_functioncalcium- and calmodulin-responsive adenylate cyclase activity
B0005576cellular_componentextracellular region
B0008294molecular_functioncalcium- and calmodulin-responsive adenylate cyclase activity
C0005576cellular_componentextracellular region
C0008294molecular_functioncalcium- and calmodulin-responsive adenylate cyclase activity
D0000086biological_processG2/M transition of mitotic cell cycle
D0000922cellular_componentspindle pole
D0002027biological_processregulation of heart rate
D0005509molecular_functioncalcium ion binding
D0005513biological_processdetection of calcium ion
D0005515molecular_functionprotein binding
D0005576cellular_componentextracellular region
D0005634cellular_componentnucleus
D0005654cellular_componentnucleoplasm
D0005737cellular_componentcytoplasm
D0005813cellular_componentcentrosome
D0005819cellular_componentspindle
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005876cellular_componentspindle microtubule
D0005886cellular_componentplasma membrane
D0005929cellular_componentcilium
D0007186biological_processG protein-coupled receptor signaling pathway
D0008076cellular_componentvoltage-gated potassium channel complex
D0010800biological_processpositive regulation of peptidyl-threonine phosphorylation
D0010801biological_processnegative regulation of peptidyl-threonine phosphorylation
D0010856molecular_functionadenylate cyclase activator activity
D0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
D0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
D0016020cellular_componentmembrane
D0016240biological_processautophagosome membrane docking
D0019855molecular_functioncalcium channel inhibitor activity
D0019901molecular_functionprotein kinase binding
D0021762biological_processsubstantia nigra development
D0030017cellular_componentsarcomere
D0030234molecular_functionenzyme regulator activity
D0031432molecular_functiontitin binding
D0031514cellular_componentmotile cilium
D0031954biological_processpositive regulation of protein autophosphorylation
D0031982cellular_componentvesicle
D0032465biological_processregulation of cytokinesis
D0032516biological_processpositive regulation of phosphoprotein phosphatase activity
D0032991cellular_componentprotein-containing complex
D0034704cellular_componentcalcium channel complex
D0035307biological_processpositive regulation of protein dephosphorylation
D0035458biological_processcellular response to interferon-beta
D0042995cellular_componentcell projection
D0043209cellular_componentmyelin sheath
D0043539molecular_functionprotein serine/threonine kinase activator activity
D0044325molecular_functiontransmembrane transporter binding
D0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
D0046872molecular_functionmetal ion binding
D0048306molecular_functioncalcium-dependent protein binding
D0050848biological_processregulation of calcium-mediated signaling
D0051343biological_processpositive regulation of cyclic-nucleotide phosphodiesterase activity
D0051592biological_processresponse to calcium ion
D0055117biological_processregulation of cardiac muscle contraction
D0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
D0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
D0060316biological_processpositive regulation of ryanodine-sensitive calcium-release channel activity
D0071346biological_processcellular response to type II interferon
D0071902biological_processpositive regulation of protein serine/threonine kinase activity
D0072542molecular_functionprotein phosphatase activator activity
D0097225cellular_componentsperm midpiece
D0098901biological_processregulation of cardiac muscle cell action potential
D0140056biological_processorganelle localization by membrane tethering
D1901842biological_processnegative regulation of high voltage-gated calcium channel activity
D1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
D1902494cellular_componentcatalytic complex
D1905913biological_processnegative regulation of calcium ion export across plasma membrane
D1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
E0000086biological_processG2/M transition of mitotic cell cycle
E0000922cellular_componentspindle pole
E0002027biological_processregulation of heart rate
E0005509molecular_functioncalcium ion binding
E0005513biological_processdetection of calcium ion
E0005515molecular_functionprotein binding
E0005576cellular_componentextracellular region
E0005634cellular_componentnucleus
E0005654cellular_componentnucleoplasm
E0005737cellular_componentcytoplasm
E0005813cellular_componentcentrosome
E0005819cellular_componentspindle
E0005829cellular_componentcytosol
E0005856cellular_componentcytoskeleton
E0005876cellular_componentspindle microtubule
E0005886cellular_componentplasma membrane
E0005929cellular_componentcilium
E0007186biological_processG protein-coupled receptor signaling pathway
E0008076cellular_componentvoltage-gated potassium channel complex
E0010800biological_processpositive regulation of peptidyl-threonine phosphorylation
E0010801biological_processnegative regulation of peptidyl-threonine phosphorylation
E0010856molecular_functionadenylate cyclase activator activity
E0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
E0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
E0016020cellular_componentmembrane
E0016240biological_processautophagosome membrane docking
E0019855molecular_functioncalcium channel inhibitor activity
E0019901molecular_functionprotein kinase binding
E0021762biological_processsubstantia nigra development
E0030017cellular_componentsarcomere
E0030234molecular_functionenzyme regulator activity
E0031432molecular_functiontitin binding
E0031514cellular_componentmotile cilium
E0031954biological_processpositive regulation of protein autophosphorylation
E0031982cellular_componentvesicle
E0032465biological_processregulation of cytokinesis
E0032516biological_processpositive regulation of phosphoprotein phosphatase activity
E0032991cellular_componentprotein-containing complex
E0034704cellular_componentcalcium channel complex
E0035307biological_processpositive regulation of protein dephosphorylation
E0035458biological_processcellular response to interferon-beta
E0042995cellular_componentcell projection
E0043209cellular_componentmyelin sheath
E0043539molecular_functionprotein serine/threonine kinase activator activity
E0044325molecular_functiontransmembrane transporter binding
E0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
E0046872molecular_functionmetal ion binding
E0048306molecular_functioncalcium-dependent protein binding
E0050848biological_processregulation of calcium-mediated signaling
E0051343biological_processpositive regulation of cyclic-nucleotide phosphodiesterase activity
E0051592biological_processresponse to calcium ion
E0055117biological_processregulation of cardiac muscle contraction
E0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
E0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
E0060316biological_processpositive regulation of ryanodine-sensitive calcium-release channel activity
E0071346biological_processcellular response to type II interferon
E0071902biological_processpositive regulation of protein serine/threonine kinase activity
E0072542molecular_functionprotein phosphatase activator activity
E0097225cellular_componentsperm midpiece
E0098901biological_processregulation of cardiac muscle cell action potential
E0140056biological_processorganelle localization by membrane tethering
E1901842biological_processnegative regulation of high voltage-gated calcium channel activity
E1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
E1902494cellular_componentcatalytic complex
E1905913biological_processnegative regulation of calcium ion export across plasma membrane
E1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
F0000086biological_processG2/M transition of mitotic cell cycle
F0000922cellular_componentspindle pole
F0002027biological_processregulation of heart rate
F0005509molecular_functioncalcium ion binding
F0005513biological_processdetection of calcium ion
F0005515molecular_functionprotein binding
F0005576cellular_componentextracellular region
F0005634cellular_componentnucleus
F0005654cellular_componentnucleoplasm
F0005737cellular_componentcytoplasm
F0005813cellular_componentcentrosome
F0005819cellular_componentspindle
F0005829cellular_componentcytosol
F0005856cellular_componentcytoskeleton
F0005876cellular_componentspindle microtubule
F0005886cellular_componentplasma membrane
F0005929cellular_componentcilium
F0007186biological_processG protein-coupled receptor signaling pathway
F0008076cellular_componentvoltage-gated potassium channel complex
F0010800biological_processpositive regulation of peptidyl-threonine phosphorylation
F0010801biological_processnegative regulation of peptidyl-threonine phosphorylation
F0010856molecular_functionadenylate cyclase activator activity
F0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
F0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
F0016020cellular_componentmembrane
F0016240biological_processautophagosome membrane docking
F0019855molecular_functioncalcium channel inhibitor activity
F0019901molecular_functionprotein kinase binding
F0021762biological_processsubstantia nigra development
F0030017cellular_componentsarcomere
F0030234molecular_functionenzyme regulator activity
F0031432molecular_functiontitin binding
F0031514cellular_componentmotile cilium
F0031954biological_processpositive regulation of protein autophosphorylation
F0031982cellular_componentvesicle
F0032465biological_processregulation of cytokinesis
F0032516biological_processpositive regulation of phosphoprotein phosphatase activity
F0032991cellular_componentprotein-containing complex
F0034704cellular_componentcalcium channel complex
F0035307biological_processpositive regulation of protein dephosphorylation
F0035458biological_processcellular response to interferon-beta
F0042995cellular_componentcell projection
F0043209cellular_componentmyelin sheath
F0043539molecular_functionprotein serine/threonine kinase activator activity
F0044325molecular_functiontransmembrane transporter binding
F0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
F0046872molecular_functionmetal ion binding
F0048306molecular_functioncalcium-dependent protein binding
F0050848biological_processregulation of calcium-mediated signaling
F0051343biological_processpositive regulation of cyclic-nucleotide phosphodiesterase activity
F0051592biological_processresponse to calcium ion
F0055117biological_processregulation of cardiac muscle contraction
F0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
F0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
F0060316biological_processpositive regulation of ryanodine-sensitive calcium-release channel activity
F0071346biological_processcellular response to type II interferon
F0071902biological_processpositive regulation of protein serine/threonine kinase activity
F0072542molecular_functionprotein phosphatase activator activity
F0097225cellular_componentsperm midpiece
F0098901biological_processregulation of cardiac muscle cell action potential
F0140056biological_processorganelle localization by membrane tethering
F1901842biological_processnegative regulation of high voltage-gated calcium channel activity
F1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
F1902494cellular_componentcatalytic complex
F1905913biological_processnegative regulation of calcium ion export across plasma membrane
F1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
Functional Information from PDB Data
site_idAC1
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA D 800
ChainResidue
DASP129
DASP131
DASP133
DGLN135
DGLU140

site_idAC2
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA D 801
ChainResidue
DGLU104
DASP93
DASP95
DASN97
DTYR99

site_idAC3
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA E 802
ChainResidue
EASP129
EASP131
EASP133
EGLN135
EGLU140

site_idAC4
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA E 803
ChainResidue
EASP93
EASP95
EASN97
ETYR99
EGLU104

site_idAC5
Number of Residues4
DetailsBINDING SITE FOR RESIDUE CA F 804
ChainResidue
FASP131
FASP133
FGLN135
FGLU140

site_idAC6
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA F 805
ChainResidue
FASP93
FASP95
FASN97
FTYR99
FGLU104

site_idAC7
Number of Residues4
DetailsBINDING SITE FOR RESIDUE YB A 901
ChainResidue
AASP491
AASP493
AHIS577
AAPC1139

site_idAC8
Number of Residues4
DetailsBINDING SITE FOR RESIDUE YB B 902
ChainResidue
BASP491
BASP493
BHIS577
BAPC2139

site_idAC9
Number of Residues4
DetailsBINDING SITE FOR RESIDUE YB C 903
ChainResidue
CASP491
CASP493
CHIS577
CAPC3139

site_idBC1
Number of Residues11
DetailsBINDING SITE FOR RESIDUE APC A 1139
ChainResidue
AARG329
ALYS346
AHIS351
ALYS353
ASER354
ALYS372
ALYS382
AGLU386
AASP493
AASN583
AYB901

site_idBC2
Number of Residues13
DetailsBINDING SITE FOR RESIDUE APC B 2139
ChainResidue
BARG329
BLYS346
BHIS351
BGLY352
BSER354
BLYS372
BLYS382
BALA490
BASP491
BASP493
BASN583
BPHE586
BYB902

site_idBC3
Number of Residues9
DetailsBINDING SITE FOR RESIDUE APC C 3139
ChainResidue
CARG329
CLYS346
CHIS351
CLYS353
CSER354
CLYS372
CASN583
CPHE586
CYB903

Functional Information from PROSITE/UniProt
site_idPS00018
Number of Residues13
DetailsEF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL
ChainResidueDetails
DASP20-LEU32
DASP56-PHE68
DASP93-LEU105
DASP129-PHE141

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues30
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:25441029, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:4UMO, ECO:0007744|PDB:4V0C, ECO:0007744|PDB:5J03
ChainResidueDetails
DLYS21
DILE27
DLEU32
DGLY23
DGLY25
EALA57
EGLY59
EGLY61
EILE63
EPHE68
FLYS21
FGLY23
FGLY25
FILE27
FLEU32
FALA57
FGLY59
FGLY61
FILE63
FPHE68
DALA57
DGLY59
DGLY61
DILE63
DPHE68
ELYS21
EGLY23
EGLY25
EILE27
ELEU32

site_idSWS_FT_FI2
Number of Residues30
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03
ChainResidueDetails
EILE130
EGLY132
EGLY134
EVAL136
EPHE141
FLYS94
FGLY96
FGLY98
FILE100
FLEU105
FILE130
FGLY132
FGLY134
FVAL136
FPHE141
DLYS94
DGLY96
DGLY98
DILE100
DLEU105
DILE130
DGLY132
DGLY134
DVAL136
DPHE141
ELYS94
EGLY96
EGLY98
EILE100
ELEU105

site_idSWS_FT_FI3
Number of Residues3
DetailsMOD_RES: N-acetylalanine => ECO:0000269|PubMed:7093203, ECO:0000269|Ref.7, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712
ChainResidueDetails
DASP2
EASP2
FASP2

site_idSWS_FT_FI4
Number of Residues3
DetailsMOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861
ChainResidueDetails
DASP22
EASP22
FASP22

site_idSWS_FT_FI5
Number of Residues3
DetailsMOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29
ChainResidueDetails
DGLU45
EGLU45
FGLU45

site_idSWS_FT_FI6
Number of Residues3
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163
ChainResidueDetails
DGLU82
EGLU82
FGLU82

site_idSWS_FT_FI7
Number of Residues3
DetailsMOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861
ChainResidueDetails
DASP95
EASP95
FASP95

site_idSWS_FT_FI8
Number of Residues3
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332
ChainResidueDetails
DILE100
EILE100
FILE100

site_idSWS_FT_FI9
Number of Residues3
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569
ChainResidueDetails
DALA102
EALA102
FALA102

site_idSWS_FT_FI10
Number of Residues3
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569
ChainResidueDetails
DASN111
EASN111
FASN111

site_idSWS_FT_FI11
Number of Residues3
DetailsMOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315
ChainResidueDetails
DLEU116
ELEU116
FLEU116

site_idSWS_FT_FI12
Number of Residues3
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332
ChainResidueDetails
DGLU139
EGLU139
FGLU139

site_idSWS_FT_FI13
Number of Residues6
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157
ChainResidueDetails
FASP22
DASP22
EASP22

218500

PDB entries from 2024-04-17

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