1RVY
E75Q MUTANT OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE, COMPLEX WITH GLYCINE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0004372 | molecular_function | glycine hydroxymethyltransferase activity |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006544 | biological_process | glycine metabolic process |
| A | 0006563 | biological_process | L-serine metabolic process |
| A | 0006565 | biological_process | L-serine catabolic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0009113 | biological_process | purine nucleobase biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0019264 | biological_process | glycine biosynthetic process from serine |
| A | 0030170 | molecular_function | pyridoxal phosphate binding |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0036094 | molecular_function | small molecule binding |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0048027 | molecular_function | mRNA 5'-UTR binding |
| A | 0050897 | molecular_function | cobalt ion binding |
| A | 0051289 | biological_process | protein homotetramerization |
| A | 0070905 | molecular_function | serine binding |
| A | 1904482 | biological_process | cellular response to tetrahydrofolate |
| A | 1990830 | biological_process | cellular response to leukemia inhibitory factor |
| B | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| B | 0004372 | molecular_function | glycine hydroxymethyltransferase activity |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006544 | biological_process | glycine metabolic process |
| B | 0006563 | biological_process | L-serine metabolic process |
| B | 0006565 | biological_process | L-serine catabolic process |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0009113 | biological_process | purine nucleobase biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0017148 | biological_process | negative regulation of translation |
| B | 0019264 | biological_process | glycine biosynthetic process from serine |
| B | 0030170 | molecular_function | pyridoxal phosphate binding |
| B | 0035999 | biological_process | tetrahydrofolate interconversion |
| B | 0036094 | molecular_function | small molecule binding |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0046653 | biological_process | tetrahydrofolate metabolic process |
| B | 0046655 | biological_process | folic acid metabolic process |
| B | 0048027 | molecular_function | mRNA 5'-UTR binding |
| B | 0050897 | molecular_function | cobalt ion binding |
| B | 0051289 | biological_process | protein homotetramerization |
| B | 0070905 | molecular_function | serine binding |
| B | 1904482 | biological_process | cellular response to tetrahydrofolate |
| B | 1990830 | biological_process | cellular response to leukemia inhibitory factor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 B 601 |
| Chain | Residue |
| A | TYR73 |
| A | GLN75 |
| A | TYR83 |
| B | SER53 |
| B | SER203 |
| B | HIS231 |
| B | LYS257 |
| B | ARG402 |
| B | PLP600 |
| site_id | AC2 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE PLG A 500 |
| Chain | Residue |
| A | SER53 |
| A | SER119 |
| A | GLY120 |
| A | SER121 |
| A | ASN124 |
| A | HIS148 |
| A | SER203 |
| A | ASP228 |
| A | ALA230 |
| A | HIS231 |
| A | THR254 |
| A | HIS256 |
| A | LYS257 |
| A | ARG402 |
| A | HOH515 |
| B | TYR73 |
| B | GLN75 |
| B | TYR83 |
| B | GLY302 |
| B | GLY303 |
| site_id | AC3 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE PLP B 600 |
| Chain | Residue |
| A | TYR73 |
| A | GLY302 |
| A | GLY303 |
| B | SER119 |
| B | GLY120 |
| B | SER121 |
| B | HIS148 |
| B | ASP228 |
| B | ALA230 |
| B | HIS231 |
| B | THR254 |
| B | HIS256 |
| B | LYS257 |
| B | PO4601 |
| B | HOH615 |
Functional Information from PROSITE/UniProt
| site_id | PS00096 |
| Number of Residues | 17 |
| Details | SHMT Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. HVvTTTTHKTLrGCRAG |
| Chain | Residue | Details |
| A | HIS249-GLY265 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000305|PubMed:7358720 |
| Chain | Residue | Details |
| A | TYR205 | |
| B | TYR205 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000250 |
| Chain | Residue | Details |
| A | LYS257 | |
| B | LYS257 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylalanine => ECO:0000269|PubMed:3553178 |
| Chain | Residue | Details |
| A | THR3 | |
| B | THR3 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: Deamidated asparagine; alternate => ECO:0000269|PubMed:2318867 |
| Chain | Residue | Details |
| A | GLY7 | |
| B | GLY7 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269|PubMed:10387080 |
| Chain | Residue | Details |
| A | THR258 | |
| B | THR258 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | CROSSLNK: Isoaspartyl glycine isopeptide (Asn-Gly); alternate => ECO:0000269|PubMed:2318867 |
| Chain | Residue | Details |
| A | GLY7 | |
| A | ALA8 | |
| B | GLY7 | |
| B | ALA8 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1dfo |
| Chain | Residue | Details |
| A | ARG81 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1dfo |
| Chain | Residue | Details |
| B | ARG81 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dfo |
| Chain | Residue | Details |
| A | GLN75 | |
| A | THR254 | |
| A | LYS257 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dfo |
| Chain | Residue | Details |
| B | GLN75 | |
| B | THR254 | |
| B | LYS257 |
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 147 |
| Chain | Residue | Details |
| A | SER74 | steric locator |
| A | GLY76 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | MET229 | electrostatic stabiliser |
| A | THR255 | electrostatic stabiliser, hydrogen bond acceptor |
| A | THR258 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | ALA264 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 147 |
| Chain | Residue | Details |
| B | SER74 | steric locator |
| B | GLY76 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | MET229 | electrostatic stabiliser |
| B | THR255 | electrostatic stabiliser, hydrogen bond acceptor |
| B | THR258 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| B | ALA264 | electrostatic stabiliser |
