1RVY

E75Q MUTANT OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE, COMPLEX WITH GLYCINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-03-03
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	114.700, 114.700, 156.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.160 - 2.900
R-factor	0.224
Rwork	0.220
R-free	0.28300
Structure solution method	Rigid body refinement of unliganded E75Q rcSHMT structure
Starting model (for MR)	1rvu
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DPS
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.200	3.060
High resolution limit [Å]	2.900	2.900
Number of reflections	22342
<I/σ(I)>	3.8	1.1
Completeness [%]	93.8	99.9
Redundancy	5.8	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	PEG 4000, KCl, KMES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.K