1RVY
E75Q MUTANT OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE, COMPLEX WITH GLYCINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-03 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.700, 114.700, 156.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.160 - 2.900 |
R-factor | 0.224 |
Rwork | 0.220 |
R-free | 0.28300 |
Structure solution method | Rigid body refinement of unliganded E75Q rcSHMT structure |
Starting model (for MR) | 1rvu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DPS |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.200 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 22342 | |
<I/σ(I)> | 3.8 | 1.1 |
Completeness [%] | 93.8 | 99.9 |
Redundancy | 5.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | PEG 4000, KCl, KMES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.K |