1RM3

Crystal structure of mutant T33A of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform, complexed with NADP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006006	biological_process	glucose metabolic process
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
B	0006006	biological_process	glucose metabolic process
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
O	0006006	biological_process	glucose metabolic process
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 O 7338

Chain	Residue
O	THR179
O	ASP181
O	ARG195
O	ARG231
O	NDP7335
O	HOH7352
O	HOH7388

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 O 7339

Chain	Residue
O	GLY209
O	ALA210
O	HOH7393
O	HOH7417
O	HOH7457
O	SER148
O	THR208

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 1338

Chain	Residue
A	THR179
A	ASP181
A	ARG195
A	ARG231
A	NDP1335

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 1339

Chain	Residue
A	SER148
A	THR150
A	THR208
A	GLY209
A	ALA210
A	HOH1362
A	HOH1372

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 2338

Chain	Residue
B	THR179
B	ASP181
B	ARG231
B	NDP2335
B	HOH2416

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 B 2339

Chain	Residue
B	SER148
B	THR208
B	GLY209
B	ALA210
B	HOH2372
B	HOH2408
B	HOH2410

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 901

Chain	Residue
B	GLU253
B	ASN256
B	ARG260

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 O 902

Chain	Residue
B	LYS72
B	HOH2344
O	PHE251
O	ALA252
O	VAL299
O	HOH7404
O	HOH7449

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 903

Chain	Residue
A	PHE251
A	ALA252
A	VAL299
A	HOH1345
O	LYS72
O	HOH7367

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 O 904

Chain	Residue
B	LYS2
B	HOH2368
O	SER247
O	LYS248
O	LYS249

---

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 905

Chain	Residue
A	SER247
A	LYS249

---

site_id	BC3
Number of Residues	27
Details	BINDING SITE FOR RESIDUE NDP O 7335

Chain	Residue
O	GLY7
O	GLY9
O	ARG10
O	ILE11
O	ASN31
O	ASP32
O	ALA33
O	GLY34
O	ARG77
O	GLY95
O	THR96
O	GLY97
O	THR119
O	ALA120
O	SER188
O	ASN313
O	TYR317
O	SO47338
O	HOH7346
O	HOH7350
O	HOH7352
O	HOH7358
O	HOH7376
O	HOH7381
O	HOH7423
O	HOH7447
O	HOH7453

---

site_id	BC4
Number of Residues	25
Details	BINDING SITE FOR RESIDUE NDP A 1335

Chain	Residue
A	ALA120
A	ASN313
A	TYR317
A	SO41338
A	HOH1360
A	HOH1369
A	HOH1374
A	HOH1394
A	HOH1395
A	HOH1401
A	HOH1417
B	SER188
B	HOH2388
B	HOH2428
A	GLY9
A	ARG10
A	ILE11
A	ASN31
A	ALA33
A	ARG77
A	GLY95
A	THR96
A	GLY97
A	PHE99
A	THR119

---

site_id	BC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NDP B 2335

Chain	Residue
B	GLY9
B	ARG10
B	ILE11
B	ASN31
B	ALA33
B	ARG77
B	GLY95
B	THR96
B	GLY97
B	PHE99
B	THR119
B	ALA120
B	ASN313
B	TYR317
B	SO42338
B	HOH2342
B	HOH2352
B	HOH2353
B	HOH2360
B	HOH2361
B	HOH2363
B	HOH2385
B	HOH2396

---

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA147-LEU154

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
O	CYS149
A	CYS149
B	CYS149

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:11846565, ECO:0000269|PubMed:12705826, ECO:0000269|PubMed:15236965, ECO:0000269|PubMed:17573533

Chain	Residue	Details
O	ARG10
B	ASP32
B	ARG77
B	ASN313
O	ASP32
O	ARG77
O	ASN313
A	ARG10
A	ASP32
A	ARG77
A	ASN313
B	ARG10

---

site_id	SWS_FT_FI3
Number of Residues	15
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
O	SER148
A	ARG231
B	SER148
B	THR179
B	ARG195
B	THR208
B	ARG231
O	THR179
O	ARG195
O	THR208
O	ARG231
A	SER148
A	THR179
A	ARG195
A	THR208

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	SITE: Activates thiol group during catalysis => ECO:0000250

Chain	Residue	Details
O	HIS176
A	HIS176
B	HIS176

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
O	CYS149
O	HIS176

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
A	CYS149
A	HIS176

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
B	CYS149
B	HIS176

---