1RM3
Crystal structure of mutant T33A of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform, complexed with NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 142.246, 185.045, 106.282 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.090 - 2.200 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jn0 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 71064 | |
<I/σ(I)> | 17.1 | 3 |
Completeness [%] | 54.0 | 98.9 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | ammonium sulphate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |