Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0009257 | biological_process | 10-formyltetrahydrofolate biosynthetic process |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0046656 | biological_process | folic acid biosynthetic process |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051870 | molecular_function | methotrexate binding |
| A | 0051871 | molecular_function | dihydrofolic acid binding |
| A | 0070401 | molecular_function | NADP+ binding |
| A | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL A 299 |
| Chain | Residue |
| A | GLY43 |
| A | HIS45 |
| A | THR46 |
| A | GLY96 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MN A 300 |
| Chain | Residue |
| A | HOH377 |
| A | GLU17 |
| A | ASN18 |
| A | ASP70 |
| A | GLY86 |
| A | HOH360 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE DHF A 161 |
| Chain | Residue |
| A | ILE5 |
| A | ALA6 |
| A | ALA7 |
| A | ASP27 |
| A | LEU28 |
| A | PHE31 |
| A | LYS32 |
| A | ARG52 |
| A | ARG57 |
| A | ILE94 |
| A | TYR100 |
| A | THR113 |
| A | HOH302 |
| A | HOH308 |
| A | HOH354 |
| A | HOH425 |
| site_id | AC4 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE DHF A 163 |
| Chain | Residue |
| A | VAL10 |
| A | ASP11 |
| A | ARG12 |
| A | HIS114 |
| A | ASP122 |
| A | HIS124 |
| A | PHE140 |
| A | CYS152 |
| A | GLU154 |
| A | HOH323 |
| A | HOH358 |
| A | HOH376 |
| A | HOH387 |
| A | HOH398 |
| A | HOH417 |
| A | HOH423 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT |
| Chain | Residue | Details |
| A | VAL13-THR35 | |
Functional Information from SwissProt/UniProt
| Chain | Residue | Details |
| A | ILE5 | |
| A | ASP27 | |
| A | ARG52 | |
| A | ARG57 | |
| A | THR113 | |
| Chain | Residue | Details |
| A | ALA7 | |
| A | VAL13 | |
| A | HIS45 | |
| A | SER63 | |
| A | LYS76 | |
| A | GLY95 | |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 7 |
| Details | Annotated By Reference To The Literature 1ra2 |
| Chain | Residue | Details |
| A | LEU54 | |
| A | LEU28 | |
| A | PHE31 | |
| A | ASP27 | |
| A | ILE5 | |
| A | MET20 | |
| A | ILE94 | |
