1RF4

Structural Studies of Streptococcus pneumoniae EPSP Synthase, Tetrahedral intermediate Bound State

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0003855	molecular_function	3-dehydroquinate dehydratase activity
A	0003866	molecular_function	3-phosphoshikimate 1-carboxyvinyltransferase activity
A	0004764	molecular_function	shikimate 3-dehydrogenase (NADP+) activity
A	0004765	molecular_function	shikimate kinase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0008652	biological_process	amino acid biosynthetic process
A	0009073	biological_process	aromatic amino acid family biosynthetic process
A	0009423	biological_process	chorismate biosynthetic process
A	0016740	molecular_function	transferase activity
A	0016765	molecular_function	transferase activity, transferring alkyl or aryl (other than methyl) groups
B	0003824	molecular_function	catalytic activity
B	0003855	molecular_function	3-dehydroquinate dehydratase activity
B	0003866	molecular_function	3-phosphoshikimate 1-carboxyvinyltransferase activity
B	0004764	molecular_function	shikimate 3-dehydrogenase (NADP+) activity
B	0004765	molecular_function	shikimate kinase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0008652	biological_process	amino acid biosynthetic process
B	0009073	biological_process	aromatic amino acid family biosynthetic process
B	0009423	biological_process	chorismate biosynthetic process
B	0016740	molecular_function	transferase activity
B	0016765	molecular_function	transferase activity, transferring alkyl or aryl (other than methyl) groups
C	0003824	molecular_function	catalytic activity
C	0003855	molecular_function	3-dehydroquinate dehydratase activity
C	0003866	molecular_function	3-phosphoshikimate 1-carboxyvinyltransferase activity
C	0004764	molecular_function	shikimate 3-dehydrogenase (NADP+) activity
C	0004765	molecular_function	shikimate kinase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0008652	biological_process	amino acid biosynthetic process
C	0009073	biological_process	aromatic amino acid family biosynthetic process
C	0009423	biological_process	chorismate biosynthetic process
C	0016740	molecular_function	transferase activity
C	0016765	molecular_function	transferase activity, transferring alkyl or aryl (other than methyl) groups
D	0003824	molecular_function	catalytic activity
D	0003855	molecular_function	3-dehydroquinate dehydratase activity
D	0003866	molecular_function	3-phosphoshikimate 1-carboxyvinyltransferase activity
D	0004764	molecular_function	shikimate 3-dehydrogenase (NADP+) activity
D	0004765	molecular_function	shikimate kinase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0008652	biological_process	amino acid biosynthetic process
D	0009073	biological_process	aromatic amino acid family biosynthetic process
D	0009423	biological_process	chorismate biosynthetic process
D	0016740	molecular_function	transferase activity
D	0016765	molecular_function	transferase activity, transferring alkyl or aryl (other than methyl) groups

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE SPQ A 1451

Chain	Residue
A	LYS20
A	ARG193
A	ASP312
A	LYS339
A	GLU340
A	ARG343
A	HIS384
A	ARG385
A	HOH1453
A	HOH1456
A	HOH1472
A	SER21
A	HOH1481
A	HOH1505
A	HOH1581
A	HOH1658
A	ARG25
A	GLY92
A	THR93
A	ARG120
A	SER166
A	ALA167
A	GLN168

---

site_id	AC2
Number of Residues	22
Details	BINDING SITE FOR RESIDUE SPQ B 1551

Chain	Residue
B	LYS20
B	SER21
B	ARG25
B	GLY92
B	THR93
B	ARG120
B	SER166
B	ALA167
B	GLN168
B	ARG193
B	ASP312
B	LYS339
B	GLU340
B	ARG343
B	HIS384
B	ARG385
B	HOH1560
B	HOH1593
B	HOH1601
B	HOH1602
B	HOH1617
B	HOH1682

---

site_id	AC3
Number of Residues	24
Details	BINDING SITE FOR RESIDUE SPQ C 1651

Chain	Residue
C	LYS20
C	SER21
C	ARG25
C	SER91
C	GLY92
C	THR93
C	ARG120
C	SER166
C	ALA167
C	GLN168
C	ARG193
C	ILE311
C	ASP312
C	LYS339
C	GLU340
C	ARG343
C	HIS384
C	ARG385
C	HOH1652
C	HOH1669
C	HOH1678
C	HOH1694
C	HOH1734
C	HOH1762

---

site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE SPQ D 1751

Chain	Residue
D	LYS20
D	SER21
D	ARG25
D	GLY92
D	THR93
D	ARG120
D	SER166
D	ALA167
D	GLN168
D	ARG193
D	ASP312
D	LYS339
D	GLU340
D	ARG343
D	HIS384
D	ARG385
D	HOH1761
D	HOH1771
D	HOH1777
D	HOH1783
D	HOH1821
D	HOH1900

---

Functional Information from PROSITE/UniProt

site_id	PS00104
Number of Residues	15
Details	EPSP_SYNTHASE_1 EPSP synthase signature 1. LNmGNSGTSIRlIsG

Chain	Residue	Details
A	LEU86-GLY100

---

site_id	PS00885
Number of Residues	19
Details	EPSP_SYNTHASE_2 EPSP synthase signature 2. KvKETDRIqVVadaLnsMG

Chain	Residue	Details
A	LYS337-GLY355

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00210, ECO:0000305|PubMed:14763973

Chain	Residue	Details
A	ASP312
B	ASP312
C	ASP312
D	ASP312

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_00210

Chain	Residue	Details
A	GLU340
B	GLU340
C	GLU340
D	GLU340

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00210, ECO:0000269|PubMed:14763973

Chain	Residue	Details
A	LYS20
D	LYS20
D	ARG25
D	LYS339
A	ARG25
A	LYS339
B	LYS20
B	ARG25
B	LYS339
C	LYS20
C	ARG25
C	LYS339

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00210

Chain	Residue	Details
A	ASN90
C	ARG120
C	ARG343
C	ARG385
D	ASN90
D	ARG120
D	ARG343
D	ARG385
A	ARG120
A	ARG343
A	ARG385
B	ASN90
B	ARG120
B	ARG343
B	ARG385
C	ASN90

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