1RF4
Structural Studies of Streptococcus pneumoniae EPSP Synthase, Tetrahedral intermediate Bound State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.495, 149.705, 88.011 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.214 |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.810 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.041 * | 0.180 * |
| Total number of observations | 822394 * | |
| Number of reflections | 103059 | |
| Completeness [%] | 97.2 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | PEG, EG, NaCl, Na/K phosphate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 10 (%) | |
| 2 | 1 | reservoir | EG | 8 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | sodium pootassium phosphate | 0.1 (M) | pH6.2 |
| 5 | 1 | reservoir | ligand | 5 (mM) | |
| 6 | 1 | drop | protein | 60 (mg/ml) | |
| 7 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 8 | 1 | drop | 100 (mM) |
