1R9Y

Bacterial cytosine deaminase D314A mutant.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004131	molecular_function	cytosine deaminase activity
A	0005829	cellular_component	cytosol
A	0006209	biological_process	cytosine catabolic process
A	0008198	molecular_function	ferrous iron binding
A	0008270	molecular_function	zinc ion binding
A	0016787	molecular_function	hydrolase activity
A	0016810	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
A	0019858	biological_process	cytosine metabolic process
A	0035888	molecular_function	isoguanine deaminase activity
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0102480	molecular_function	5-fluorocytosine deaminase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE A 501

Chain	Residue
A	HIS61
A	HIS63
A	HIS214
A	ASP313
A	MG502
A	HOH604

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site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 502

Chain	Residue
A	HOH603
A	HOH604
A	HOH605
A	HOH606
A	FE501
A	HOH601
A	HOH602

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site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 503

Chain	Residue
A	ALA128
A	LYS134
A	GLU171
A	ARG174
A	LEU175
A	HOH798
A	HOH984
A	HOH984
A	HOH1026

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:11812140, ECO:0000305|PubMed:21545144, ECO:0000305|PubMed:21604715

Chain	Residue	Details
A	GLU217

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site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:21545144, ECO:0000269|PubMed:21604715, ECO:0000269|Ref.14

Chain	Residue	Details
A	HIS61
A	HIS63
A	HIS214
A	ASP313

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site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11812140, ECO:0000269|PubMed:15381761, ECO:0000269|PubMed:21545144, ECO:0000269|PubMed:21604715

Chain	Residue	Details
A	GLN156

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site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11812140, ECO:0000269|PubMed:15381761, ECO:0000269|PubMed:21545144

Chain	Residue	Details
A	TRP319

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site_id	SWS_FT_FI5
Number of Residues	1
Details	SITE: Activates the nucleophilic water => ECO:0000305|PubMed:21545144, ECO:0000305|PubMed:21604715

Chain	Residue	Details
A	HIS246

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 710

Chain	Residue	Details
A	HIS61	metal ligand
A	HIS63	metal ligand
A	GLN156	electrostatic stabiliser
A	HIS214	metal ligand
A	GLU217	proton acceptor, proton donor
A	ASP313	metal ligand

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