1QR6
HUMAN MITOCHONDRIAL NAD(P)-DEPENDENT MALIC ENZYME
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004470 | molecular_function | malic enzyme activity |
| A | 0004471 | molecular_function | malate dehydrogenase (decarboxylating) (NAD+) activity |
| A | 0004473 | molecular_function | malate dehydrogenase (decarboxylating) (NADP+) activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0006090 | biological_process | pyruvate metabolic process |
| A | 0006108 | biological_process | malate metabolic process |
| A | 0008948 | molecular_function | oxaloacetate decarboxylase activity |
| A | 0009055 | molecular_function | electron transfer activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051287 | molecular_function | NAD binding |
| A | 1902031 | biological_process | regulation of NADP metabolic process |
| B | 0004470 | molecular_function | malic enzyme activity |
| B | 0004471 | molecular_function | malate dehydrogenase (decarboxylating) (NAD+) activity |
| B | 0004473 | molecular_function | malate dehydrogenase (decarboxylating) (NADP+) activity |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0006090 | biological_process | pyruvate metabolic process |
| B | 0006108 | biological_process | malate metabolic process |
| B | 0008948 | molecular_function | oxaloacetate decarboxylase activity |
| B | 0009055 | molecular_function | electron transfer activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051287 | molecular_function | NAD binding |
| B | 1902031 | biological_process | regulation of NADP metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE NAD A 601 |
| Chain | Residue |
| A | ARG165 |
| A | PHE344 |
| A | ASP345 |
| A | LYS346 |
| A | VAL392 |
| A | ALA393 |
| A | GLY394 |
| A | LEU398 |
| A | LEU419 |
| A | SER420 |
| A | ASN421 |
| A | ASN259 |
| A | GLY446 |
| A | GLY465 |
| A | ASN467 |
| A | HOH2008 |
| A | HOH2048 |
| A | HOH2275 |
| A | HOH2398 |
| A | THR283 |
| A | LEU310 |
| A | GLY311 |
| A | ALA312 |
| A | GLY313 |
| A | GLU314 |
| A | ALA315 |
| site_id | AC2 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE NAD A 602 |
| Chain | Residue |
| A | HIS154 |
| A | LYS156 |
| A | GLY192 |
| A | ILE193 |
| A | ARG194 |
| A | ARG197 |
| A | ILE479 |
| A | LEU480 |
| A | ASN482 |
| A | ARG542 |
| A | ARG556 |
| A | HOH2233 |
| A | HOH2301 |
| A | HOH2302 |
| A | HOH2324 |
| A | HOH2510 |
| A | HOH2542 |
| A | HOH2631 |
| B | THR1243 |
| B | ASP1244 |
| B | ARG1248 |
| B | ARG1484 |
| site_id | AC3 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE NAD B 1601 |
| Chain | Residue |
| B | ARG1165 |
| B | ASN1259 |
| B | THR1283 |
| B | GLY1311 |
| B | ALA1312 |
| B | GLY1313 |
| B | GLU1314 |
| B | ALA1315 |
| B | ASP1345 |
| B | LYS1346 |
| B | VAL1392 |
| B | ALA1393 |
| B | GLY1394 |
| B | ALA1395 |
| B | LEU1419 |
| B | SER1420 |
| B | ASN1421 |
| B | GLY1446 |
| B | GLY1465 |
| B | ASN1467 |
| B | HOH2035 |
| B | HOH2046 |
| B | HOH2093 |
| B | HOH2173 |
| B | HOH2326 |
| B | HOH2673 |
| site_id | AC4 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE NAD B 1602 |
| Chain | Residue |
| A | THR243 |
| A | ASP244 |
| A | GLY247 |
| A | ARG248 |
| A | ARG484 |
| B | HIS1154 |
| B | LYS1156 |
| B | GLY1192 |
| B | ILE1193 |
| B | ARG1194 |
| B | ARG1197 |
| B | ILE1479 |
| B | LEU1480 |
| B | ASN1482 |
| B | ARG1542 |
| B | ARG1556 |
| B | HOH2017 |
| B | HOH2129 |
| B | HOH2352 |
| B | HOH2676 |
| B | HOH2721 |
Functional Information from PROSITE/UniProt
| site_id | PS00331 |
| Number of Residues | 17 |
| Details | MALIC_ENZYMES Malic enzymes signature. FnDDiqGTAaVaLAGLL |
| Chain | Residue | Details |
| A | PHE276-LEU292 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000269|PubMed:12962632 |
| Chain | Residue | Details |
| A | TYR112 | |
| B | TYR1112 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000269|PubMed:12962632 |
| Chain | Residue | Details |
| A | LYS183 | |
| B | LYS1183 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12121650, ECO:0000269|PubMed:12962632 |
| Chain | Residue | Details |
| A | ARG67 | |
| A | ARG91 | |
| B | ARG1067 | |
| B | ARG1091 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12962632 |
| Chain | Residue | Details |
| A | ARG165 | |
| A | ASN421 | |
| A | ASN466 | |
| B | ARG1165 | |
| B | ASN1421 | |
| B | ASN1466 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10700286, ECO:0000269|PubMed:12121650, ECO:0000269|PubMed:12962632 |
| Chain | Residue | Details |
| A | GLU255 | |
| A | ASP256 | |
| A | ASP279 | |
| B | GLU1255 | |
| B | ASP1256 | |
| B | ASP1279 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10700286, ECO:0000269|PubMed:12962632 |
| Chain | Residue | Details |
| A | ASN259 | |
| A | GLY311 | |
| B | ASN1259 | |
| B | GLY1311 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 10 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS156 | |
| B | LYS1346 | |
| A | LYS224 | |
| A | LYS240 | |
| A | LYS272 | |
| A | LYS346 | |
| B | LYS1156 | |
| B | LYS1224 | |
| B | LYS1240 | |
| B | LYS1272 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1do8 |
| Chain | Residue | Details |
| A | ASP278 | |
| A | LYS183 | |
| A | TYR112 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1do8 |
| Chain | Residue | Details |
| B | LYS1183 | |
| B | ASP1278 | |
| B | TYR1112 |
| site_id | MCSA1 |
| Number of Residues | 8 |
| Details | M-CSA 21 |
| Chain | Residue | Details |
| A | TYR112 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | ARG165 | electrostatic stabiliser, hydrogen bond donor |
| A | LYS183 | electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| A | GLU255 | metal ligand |
| A | ASP256 | metal ligand |
| A | ASP278 | hydrogen bond acceptor, proton acceptor, proton donor |
| A | ASP279 | metal ligand |
| A | ASN421 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 8 |
| Details | M-CSA 21 |
| Chain | Residue | Details |
| B | TYR1112 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | ARG1165 | electrostatic stabiliser, hydrogen bond donor |
| B | LYS1183 | electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| B | GLU1255 | metal ligand |
| B | ASP1256 | metal ligand |
| B | ASP1278 | hydrogen bond acceptor, proton acceptor, proton donor |
| B | ASP1279 | metal ligand |
| B | ASN1421 | electrostatic stabiliser, hydrogen bond donor |
