1QR6
HUMAN MITOCHONDRIAL NAD(P)-DEPENDENT MALIC ENZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-10-05 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | B 1 1 2 |
Unit cell lengths | 204.400, 107.000, 59.200 |
Unit cell angles | 90.00, 90.00, 101.90 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.28700 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MADSYS |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.043 | 0.108 |
Total number of observations | 343811 * | |
Number of reflections | 70337 | |
<I/σ(I)> | 20 | |
Completeness [%] | 90.0 | 69 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | 100 MM HEPES, 8% PEG 8000, 5% MPD, 6 MM MGSO4, 10 MM TARTRONATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | 100 MM HEPES, 8% PEG 8000, 5% MPD, 6 MM MGSO4, 10 MM TARTRONATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | 100 MM HEPES, 8% PEG 8000, 5% MPD, 6 MM MGSO4, 10 MM TARTRONATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
10 | 1 | reservoir | tartronate | 10 (mM) | |
2 | 1 | drop | Tris | 30 (mM) | |
3 | 1 | drop | 125 (mM) | ||
4 | 1 | drop | NAD+ | 3 (mM) | |
5 | 1 | drop | dithiothreitol | 10 (mM) | |
6 | 1 | reservoir | HEPES | 100 (mM) | |
7 | 1 | reservoir | PEG8000 | 8 (%) | |
8 | 1 | reservoir | MPD | 5 (%) | |
9 | 1 | reservoir | 6 (mM) |