1QHP
FIVE-DOMAIN ALPHA-AMYLASE FROM BACILLUS STEAROTHERMOPHILUS, MALTOSE COMPLEX
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004556 | molecular_function | alpha-amylase activity |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0030246 | molecular_function | carbohydrate binding |
| A | 0043169 | molecular_function | cation binding |
| A | 2001070 | molecular_function | starch binding |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P13507 |
| Chain | Residue | Details |
| A | ASP228 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor => ECO:0000250|UniProtKB:P13507 |
| Chain | Residue | Details |
| A | GLU256 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 15 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10387084, ECO:0007744|PDB:1QHO |
| Chain | Residue | Details |
| A | ASP21 | |
| A | GLU101 | |
| A | GLU102 | |
| A | ASN131 | |
| A | GLN184 | |
| A | ASP198 | |
| A | HIS232 | |
| A | ASP23 | |
| A | ASN26 | |
| A | ASN27 | |
| A | GLY48 | |
| A | ASP50 | |
| A | ASP76 | |
| A | ASN77 | |
| A | ASP79 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 7 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P0C1B3 |
| Chain | Residue | Details |
| A | TRP93 | |
| A | HIS132 | |
| A | ARG226 | |
| A | LYS231 | |
| A | GLY259 | |
| A | ASP329 | |
| A | ARG376 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | SITE: Transition state stabilizer => ECO:0000250|UniProtKB:P0C1B3 |
| Chain | Residue | Details |
| A | ASP329 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 905 |
| Chain | Residue | Details |
| A | ASP228 | covalent catalysis |
| A | GLU256 | proton shuttle (general acid/base) |
| A | ASP329 | transition state stabiliser |
