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1QHP

FIVE-DOMAIN ALPHA-AMYLASE FROM BACILLUS STEAROTHERMOPHILUS, MALTOSE COMPLEX

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]100
Detector technologyIMAGE PLATE
DetectorMARRESEARCH
Spacegroup nameP 31 2 1
Unit cell lengths89.760, 89.760, 185.710
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution20.000 - 1.700
Rwork0.153
R-free0.19100
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1cdg
RMSD bond length0.008
RMSD bond angle0.022
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.730
High resolution limit [Å]1.7001.700
Rmerge0.0800.450
Total number of observations460513

*

Number of reflections87912

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<I/σ(I)>17.83.1
Completeness [%]95.892.5
Redundancy5.32.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

6drop consists of 1:1 mixture of well and protein solutions

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropenzyme19 (mg/ml)
21dropTris-HCl10 (mM)
31drop0.2 (M)
41drop5 (mM)
51reservoir0.9 (M)
61reservoirPEG14502.5 (%(w/v))
71reservoirTEA50 (mM)
81reservoirmaltose100 (mM)

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PDB entries from 2024-09-18

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