1Q5M
Binary complex of rabbit 20alpha-hydroxysteroid dehydrogenase with NADPH
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001516 | biological_process | prostaglandin biosynthetic process |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006631 | biological_process | fatty acid metabolic process |
| A | 0006633 | biological_process | fatty acid biosynthetic process |
| A | 0006693 | biological_process | prostaglandin metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0047006 | molecular_function | 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase [NAD(P)+] activity |
| A | 0050221 | molecular_function | prostaglandin E2 9-reductase activity |
| B | 0001516 | biological_process | prostaglandin biosynthetic process |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006631 | biological_process | fatty acid metabolic process |
| B | 0006633 | biological_process | fatty acid biosynthetic process |
| B | 0006693 | biological_process | prostaglandin metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0047006 | molecular_function | 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase [NAD(P)+] activity |
| B | 0050221 | molecular_function | prostaglandin E2 9-reductase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1001 |
| Chain | Residue |
| A | SER11 |
| A | ASP12 |
| A | TYR184 |
| A | LYS185 |
| A | HOH1026 |
| A | HOH1256 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1002 |
| Chain | Residue |
| B | LYS185 |
| B | HOH1111 |
| B | HOH1312 |
| B | HOH1401 |
| B | SER11 |
| B | ASP12 |
| B | TYR184 |
| site_id | AC3 |
| Number of Residues | 35 |
| Details | BINDING SITE FOR RESIDUE NDP A 1003 |
| Chain | Residue |
| A | GLY22 |
| A | THR23 |
| A | TYR24 |
| A | ASP50 |
| A | TYR55 |
| A | HIS117 |
| A | SER166 |
| A | ASN167 |
| A | GLN190 |
| A | TYR216 |
| A | SER217 |
| A | ALA218 |
| A | LEU219 |
| A | GLY220 |
| A | SER221 |
| A | HIS222 |
| A | LEU236 |
| A | ALA253 |
| A | LEU268 |
| A | LYS270 |
| A | SER271 |
| A | PHE272 |
| A | THR273 |
| A | ARG276 |
| A | GLU279 |
| A | ASN280 |
| A | HOH1009 |
| A | HOH1029 |
| A | HOH1061 |
| A | HOH1064 |
| A | HOH1230 |
| A | HOH1279 |
| A | HOH1280 |
| A | HOH1300 |
| A | HOH1310 |
| site_id | AC4 |
| Number of Residues | 37 |
| Details | BINDING SITE FOR RESIDUE NDP B 1004 |
| Chain | Residue |
| B | GLY22 |
| B | THR23 |
| B | TYR24 |
| B | ASP50 |
| B | TYR55 |
| B | LYS84 |
| B | HIS117 |
| B | SER166 |
| B | ASN167 |
| B | GLN190 |
| B | TYR216 |
| B | SER217 |
| B | ALA218 |
| B | LEU219 |
| B | GLY220 |
| B | SER221 |
| B | HIS222 |
| B | ALA253 |
| B | LEU268 |
| B | LYS270 |
| B | SER271 |
| B | PHE272 |
| B | THR273 |
| B | ARG276 |
| B | GLU279 |
| B | ASN280 |
| B | HOH1010 |
| B | HOH1029 |
| B | HOH1118 |
| B | HOH1130 |
| B | HOH1167 |
| B | HOH1292 |
| B | HOH1310 |
| B | HOH1322 |
| B | HOH1323 |
| B | HOH1332 |
| B | HOH1360 |
Functional Information from PROSITE/UniProt
| site_id | PS00062 |
| Number of Residues | 18 |
| Details | ALDOKETO_REDUCTASE_2 Aldo/keto reductase family signature 2. MekckdaglAKSIGVSNF |
| Chain | Residue | Details |
| A | MET151-PHE168 |
| site_id | PS00063 |
| Number of Residues | 16 |
| Details | ALDOKETO_REDUCTASE_3 Aldo/keto reductase family putative active site signature. LAKSFTekRIkENiQV |
| Chain | Residue | Details |
| A | LEU268-VAL283 |
| site_id | PS00798 |
| Number of Residues | 18 |
| Details | ALDOKETO_REDUCTASE_1 Aldo/keto reductase family signature 1. GFRHIDSAyfyknEkeVG |
| Chain | Residue | Details |
| A | GLY45-GLY62 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton donor"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 38 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"15123423","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Site: {"description":"Required for substrate specificity"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Site: {"description":"Lowers pKa of active site Tyr","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mrq |
| Chain | Residue | Details |
| A | LYS84 | |
| A | HIS117 | |
| A | ASP50 | |
| A | TYR55 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mrq |
| Chain | Residue | Details |
| B | LYS84 | |
| B | HIS117 | |
| B | ASP50 | |
| B | TYR55 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mrq |
| Chain | Residue | Details |
| A | LYS84 | |
| A | TYR55 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mrq |
| Chain | Residue | Details |
| B | LYS84 | |
| B | TYR55 |
