1Q5M
Binary complex of rabbit 20alpha-hydroxysteroid dehydrogenase with NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2001-04-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.540, 85.270, 66.150 |
Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
Resolution | 57.740 - 1.320 |
R-factor | 0.14918 |
Rwork | 0.148 |
R-free | 0.17690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.033 |
RMSD bond angle | 2.607 |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.740 | 1.370 |
High resolution limit [Å] | 1.320 | 1.320 |
Rmerge | 0.069 | |
Number of reflections | 141980 | |
<I/σ(I)> | 15.1 | |
Completeness [%] | 69.1 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | LiSO4 and PEG 4K, pH 7.5, VAPOR DIFFUSION |